Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING finger protein Unkempt doc

... or Rac1 N17 on nuclear accumulation of Unkempt (not shown). However, taken together, these data support the idea that Rac and Unkempt can translocate in the nuclear compartment and activate BAF60b ... arises of the cellular compartment where this process takes place. Indeed, although Rac activation is believed to occur primarily at the plasma membrane, BAF60b, as w...

Ngày tải lên: 06/03/2014, 09:22

... are Mycobacterium tuber- culosis (185 proteins) and Chlamydophila caviae (13 proteins), while in archaea the top and bottom is rep- resented by Haloarcula marismortui (146 proteins) and Nanoarchaeum ... In Thermoplasma volcanium, Pyrococcus horikoshii, Thermococcus kodakaraensis, Candida glabrata and Yarrowia lipolytica, the Ross- mann proteins are two to three times more redund...

Ngày tải lên: 23/03/2014, 10:21

... strand exchange assay were synthesized by Metabion (Martinsreid, Germany) with the following sequences: PhiC: 5¢-CGATACGCTCAAAGTCA AAATAATCAGCGTGACATTCAGAAGGGTAATAAG AACG-3¢;, PhiW: 5¢-CGTTCTTATTACCCTTCTGAA TGTCACGCTGATTATTTTGACTTTGAGCGTATCG-3¢ and ... 5¢-CGTTCTTATTACCCTTCTGAA TGTCACGCTGATTATTTTGACTTTGAGCGTATCG-3¢ and M13C: 5¢-CTACAACGCCTGTAGCATTCCACAGA CAGCCCTCATAGTTAGCGTAACGAGATCG-3¢....

Ngày tải lên: 19/02/2014, 07:20

... of the peptidase to hydrolyse Ala-Ala-pNA and Ala-pNA (or other chromogenic amino acids) at reason- able rates clearly indicates exclusive cleavage of the anilide bond of Ala-Ala-Val-Ala-pNA. ... exhibiting precisely the sequence of FRAP-TGase. Yellowing of the Ala-Ala-Val-Ala-pNA solution must be the result of direct cleavage of the anilide bond. Ala-pNA and Ala-Ala-pNA were...

Ngày tải lên: 31/03/2014, 07:20

... toward cleavage of the GUC 665 containing sequence) is 5¢-CGGTTCGAAACCGGGCACTACAAA AACCAACTTTGCCCTGCCCCCTGATGAGGCCGA AAGGCCGAAACTTGCCCCTGGTACCCCGGATAT CTTTTTTTCTATCGCGTCGACCT-3¢ and the template encoding ... (targeted toward CUC 825 containing sequence) is 5¢-CGGTTCGAAACCGGGCACTACAAA AACCAACTTTCACCCTTCCGCTGATGAGGCCGA AAGGCCGAAAGGTCCCGGTGGTACCCCGGATA TCTTTTTTTCTATCGCGTCGACCT-3¢. The...

Ngày tải lên: 08/03/2014, 08:20

... Kun Zou 1 , Wataru Araki 3 , Chiaki Tanabe 1 , Naoko Yagishita 4 , Yoshihisa Yamano 4 , Tetsuya Amano 4 , Makoto Michikawa 2 , Toshihiro Nakajima 4,5,6 and Hiroto Komano 1 1 Department of Neuroscience, ... of Japan, and by a grant from the Ministry of Health, Labor and Welfare of Japan. We thank Dr Paul Lang- man for assistance with the English. References 1 Selkoe DJ (2002) Decip...

Ngày tải lên: 23/03/2014, 04:20

... formation at the terminator site. When the adjacent uracil-rich sequence is being transcribed, the affinity of the dA–U base pairs is insufficient to stabil- ize the DNA–RNA duplex, and the transcript, together ... (Bc-Csp) and Thermotoga maritima (Tm-Csp). The peptide chains of the CSP are arranged as five antiparallel b strands, separated by four loops and folded into...

Ngày tải lên: 23/03/2014, 09:21

... complete plant transformation and has the advantages of stable transgenic material over transient assays, in which damage often occurs during DNA incorporation and for which there is variability in the ... 5¢-C CCCGGG AGTGAAAAAAGCAAAGTTCAAC-3¢; SPS-2 (105): 5¢-CCC CCATGGCTCCTCCAAATGATTTTATATC-3¢. The underlined sequences indicate the EcoRI (5038), BamHI (PG3B), NcoI (EPS-1 and...

Ngày tải lên: 31/03/2014, 07:20

... Each approach has its advantages, and the choice of which to employ will largely depend on the available starting enzyme and the extent of existing structural and mech- anistic characterization ... between His138 and glyphosate and activation of the substrate amine. This substrate-assisted proton transfer mechanism is consistent with the observed pH dependence of k cat ,...

Ngày tải lên: 14/02/2014, 14:20

... normalize for loading. Statistical analysis All values are expressed as mean ± standard deviation. Statistical significance was determined using a one-way HSS and mitochondrial permeability transition ... and apoptosis [41,44]. Apoptosis is a genetically predetermined mechanism that may be activated by several molecular pathways. The best characterized and the most prominent pa...

Ngày tải lên: 16/02/2014, 09:20