Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf

... MINIREVIEW Alternative splicing: good and bad effects of translationally silent substitutions M. Raponi and D. Baralle Academic Unit of Genetic Medicine, Human Genetics Division, University of Southampton, ... in the alternative splicing (AS) isoforms (that become substrates of both positive and negative natural selection). Two main categories of translation...

Ngày tải lên: 06/03/2014, 09:22

... doi:10.1111/j.1742-4658.2009.07518.x. 72 Raponi M & Baralle D. (2009). Alternative Splicing: Translationally silent substitutions that affect splicing: the bad and the good. FEBS J, doi:10.1111/j.1742- 4658.2009.07519.x. 73 ... 855 MINIREVIEW Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies Ashish Dhir and Emanuele...

Ngày tải lên: 06/03/2014, 09:22

... generation of ever larger sequence datasets, the proportion of multi-exon human genes that are known to be alternatively spliced has expanded to 92–94%, of which 85% have a minor isoform frequency of ... regu- lated in striated muscle and neurons [2,27,75], consis- tent with the expression patterns of FOX1 and -2. Analysis of breast and ovarian cancer using an RT- PCR panel...

Ngày tải lên: 06/03/2014, 09:22

... Disruption of ESSV results in increased vpr mRNA accumulation and exon 3 inclusion, decreased accumulation of unspliced viral mRNA and decreased Fig. 1. Organization of HIV-1 genome and different ... the consensus because of their low content of pyrimidine nucleotides. A B Fig. 4. Positive and negative regulation of HIV-1 3¢ss. (A) Action of SR proteins as positive regul...

Ngày tải lên: 06/03/2014, 09:22

... subunits, P-, T- and L-protein. As a result of the GDC reaction cycle, which requires the coenzymes NAD + and tetrahydro- folate, methylene-tetrahydrofolate and NADH are generated and CO 2 and NH 3 are ... combination with use of lipoate-specific antibodies, and further confirmed by MALDI-TOF MS (Fig. 2). Leaf mitochondria contain large amounts of all GDC subunits and provid...

Ngày tải lên: 23/03/2014, 04:20

... shift of the proton, and Dd N is the change in the chemical shift of the nitrogen [56]. Modeling and comparison of the structures of PSs dali [57] was used to superimpose the crystal structures of PSs ... the structures of PSs from E. coli and M. tuberculosis. Structural analyses Analysis of the sites of substrate binding from NMR data, and structural comparison of t...

Ngày tải lên: 16/02/2014, 09:20

... 7.0 and 4 °C for indicated periods of time. Lane c, the nicked precursor was incubated at pH 7.0 and 4 °C for 24 h and then dialyzed overnight at 4 °C and pH 4.0. (B) Effects of addition of different ... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensitie...

Ngày tải lên: 19/02/2014, 07:20

... contribution of each feature type to the overall performance of the sys- tem, we run a number of ablation tests. An ablation test consists of removing one feature of the system at a time and re-evaluating ... for the perfor- mance of our system, we calculate the average corre- lation between the CLC and the examiners’ scores, and find an upper bound of 0.796 and 0.792 Pea...

Ngày tải lên: 20/02/2014, 04:20

... 38] Y/S library in all the CDRs of heavy chain and CDR3 of light chain [ref 39] 10 FN3 Randomization in CDR loops of heavy and light chains Randomization in BC, DE and FG loops Fv Fig. 4. Local ... immunogenicity of the antibody framework from a different species [23–25]. The success of the series of studies shows that the stable framework structure of Fv enables the trans...

Ngày tải lên: 06/03/2014, 11:20

... layer of vinyl + 3 layers of curing blanket + roof 20 days (a) Temperature analysis of the total cross section of mat foundation (b) Stress dispersion of the total cross section of ... during and after curing (c) TCI of the total cross section of mat foundation during and after curing Figure 5. Full-Scale thermal stress and TCI of mat foundation 4....

Ngày tải lên: 07/03/2014, 03:20