Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf

... MINIREVIEW Alternative splicing: good and bad effects of translationally silent substitutions M. Raponi and D. BaralleAcademic Unit of Genetic Medicine, Human Genetics Division, University of Southampton, ... in the alternative splicing (AS) isoforms (thatbecome substrates of both positive and negativenatural selection).Two main categories of translationally silent varia-tions can alter splicing: ... alterations in this processmay occur as a consequence of translationally silent mutations leading tothe expression of novel splicing isoforms and ⁄ or loss of an existing one.This phenomenon can either...

Báo cáo khoa học: Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies pot

... doi:10.1111/j.1742-4658.2009.07518.x.72 Raponi M & Baralle D. (2009). Alternative Splicing: Translationally silent substitutions that affect splicing: the bad and the good. FEBS J, doi:10.1111/j.1742-4658.2009.07519.x.73 ... 855MINIREVIEW Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategiesAshish Dhir and Emanuele BurattiInternational Centre for Genetic Engineering and Biotechnology ... (2006) Alternative splicing: new insightsfrom global analyses. Cell 126, 37–47.20 Tarrio R, Ayala FJ & Rodriguez-Trelles F (2008) Alternative splicing: a missing piece in the puzzle of intron...

Báo cáo khoa học: Alternative splicing: global insights potx

... generation of ever larger sequence datasets, theproportion of multi-exon human genes that are knownto be alternatively spliced has expanded to 92–94%, of which 85% have a minor isoform frequency of ... regu-lated in striated muscle and neurons [2,27,75], consis-tent with the expression patterns of FOX1 and -2.Analysis of breast and ovarian cancer using an RT-PCR panel of alternative splicing events ... the investigation of alternative splicing at a global scale. Splice-sensitive microarray platforms and deep sequencing allow quantitative proﬁling of very large numbers of alternative splicing...

Báo cáo khoa học: Alternative splicing: regulation of HIV-1 multiplication as a target for therapeutic action docx

... Disruption of ESSV results in increased vprmRNA accumulation and exon 3 inclusion, decreasedaccumulation of unspliced viral mRNA and decreasedFig. 1. Organization of HIV-1 genome and different ... theconsensus because of their low content of pyrimidine nucleotides.ABFig. 4. Positive and negative regulation of HIV-1 3¢ss. (A) Action of SR proteins as positive regulators. (B) Action of hnRNP proteins ... multimeriza-tion of hnRNP A1 towards the A3 3¢ss leads to theocclusion of the PPT and to site A3 inhibition [36].Enzymatic and chemical probing revealed theoccurrence of several SC35 and SRp40 binding...

Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc

... subunits, P-, T- and L-protein. As a result of the GDC reaction cycle,which requires the coenzymes NAD+ and tetrahydro-folate, methylene-tetrahydrofolate and NADH aregenerated and CO2 and NH3are ... combination with use of lipoate-speciﬁc antibodies, and further conﬁrmed byMALDI-TOF MS (Fig. 2).Leaf mitochondria contain large amounts of allGDC subunits and provide a convenient source of native P-protein. ... nitrogen after addition of 20% v ⁄ vglycerol and stored at )80 °C.MALDI-TOF MSThe purity of recombinant proteins was assessed by SDS–PAGE [30], and the molecular identity of all H-proteinsincluding...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... shift of the proton, and DdNis thechange in the chemical shift of the nitrogen [56].Modeling and comparison of the structures of PSsdali [57] was used to superimpose the crystal structures of PSs ... thestructures of PSs from E. coli and M. tuberculosis.Structural analysesAnalysis of the sites of substrate binding from NMR data, and structural comparison of the E. coli, M. tuberculosis and A. ... control, and that thisallosteric control arises from the homotropic effects of one of the substrates, i.e. pantoate [8,9]. One of theinteresting features of the plant PSs is the presence of a conserved...

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... 7.0 and 4 °C for indicated periods of time. Lane c, the nicked precursor was incubated at pH 7.0 and 4 °C for 24 h and then dialyzed overnight at 4 °C and pH 4.0. (B) Effects of addition of different ... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and ... time-dependent enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed bySDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa bandsdid not disappear completely...

Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf

... contribution of eachfeature type to the overall performance of the sys-tem, we run a number of ablation tests. An ablationtest consists of removing one feature of the systemat a time and re-evaluating ... for the perfor-mance of our system, we calculate the average corre-lation between the CLC and the examiners’ scores, and ﬁnd an upper bound of 0.796 and 0.792 Pear-son’s and Spearman’s correlation ... Processing.Eleni Miltsakaki and Karen Kukich. 2000. Automatedevaluation of coherence in student essays. In Proceed-ings of LREC 2000.Eleni Miltsakaki and Karen Kukich. 2004. Evaluation of text coherence...

Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx

... 38]Y/S libraryin all the CDRs of heavy chain and CDR3 of light chain[ref 39]10FN3Randomization in CDR loops of heavy and light chainsRandomizationin BC, DE and FG loopsFvFig. 4. Local ... immunogenicity of the antibody framework froma different species [23–25]. The success of the series of studies shows that the stable framework structure of Fv enables the transfer of function by means of ... chainHeavychainLightchainInsertbetweenThr25 and TOPNCSVHHGlu26FvFig. 2. Functionalization of small scaffold proteins by replacing a loop of the scaffold protein with a CDR loop of antibody fragments.(A) Replacement of the candidate...

Báo cáo khoa học "ADIABATIC TEMPERATURE RISE AND REACTION RATE OF MASS STRUCTURE IN LOTTE CENTER HANOI PROJECT " pptx

... layer of vinyl + 3 layers of curing blanket + roof 20 days (a) Temperature analysis of the total cross section of mat foundation (b) Stress dispersion of the total cross section of ... during and after curing (c) TCI of the total cross section of mat foundation during and after curing Figure 5. Full-Scale thermal stress and TCI of mat foundation 4. Hydration history of mat ... of the Mock-up Figure 2. 5.7m ⅹ 5.7m ⅹ 5.7m location of hydration heat sensor and image 3. Analysis of internal restraining stress for mat foundation 3.1 The amount of hydration heat and...

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