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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... FEBS Proteolytic activation and function of the cytokine Spa¨tzle in the innate immune response of a lepidopteran insect, Manduca sexta Chunju An1, Haobo Jiang2 and Michael R. Kanost11 Department ... development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa¨tzle in a lepidopteran insect, Manduca sexta, in which hemolymph proteinases activated ... aegypti, An. gambiae, B. mori, M. sexta, Naso-nia vitripennis, and T. castaneum. We could not includeAn. gambiae Spa¨tzle-1 in the analysis, because the Manduca sexta Spa¨tzle C. An et al.150...
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Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx

Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx

... (reductionindicated as dashed pattern) by NADH (via FMN-b) induces aninstantaneous activation of the Ni-Fe site (removal of the OH–ligandfrom nickel) enabling the interaction with H2.AslongasFMN-aispresent, ... total loss of thisactivity when assayed in the usual way, i.e. after addition of H2, a catalytic amount of NADH and the subsequentaddition of BV. Restoration of this activity (to 88%) alsorequired ... from Sigma) as a standard, in a Shimadzu RF-5001PC spectrofluorimeter (Kyoto, Japan). The concentration of the standard (in a buffer solution of pH 6.9) was calculated from the difference in absorption...
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Báo cáo khoa học:

Báo cáo khoa học: "Combining Statistical and Knowledge-based Spoken Language Understanding in Conditional Models" pptx

... practices in language engineering for every new domain. On the other hand, a statistical learning approach needs a large amount of annotated data for model training, which is seldom available in ... expertise in natural language processing and speech recognition, and best practices in language engineering for every new domain. In the past decade many statistical learning approaches have been ... practical applications outside of large research labs. A generative HMM/CFG composite model, which integrates easy-to-obtain domain knowledge into a data-driven statistical learning framework,...
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Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... into two fragments: the A chaincorresponding to the catalytic domain (C domain); and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The C and ... triggers a conformational change, leading toinsertion of the toxin in the membrane. The C domainis then translocated across the endosomal membraneinto the cytosol. The C domain ADP-ribosylates the elongation ... aggregate at high concen-trations, etc. In the case of the T domain, the MGstate corresponds to the functional state, which initi-ates the translocation of the catalytic domain. Here, the data allowed...
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Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

... by:FD¼ A A N =A A NWhere A Nis the absorbance of the protein sample at 30 °C, A Dis the absorbance of the sample at 80 °C and A Tis the absorbance of the protein sample at different ... wasincubated with 10 mM glycine and 500 lM FTHF for 5 min and thendialyzed against buffer D not containing glycine and FTHF. Such anenzyme was once again incubated with glycine (10 mM) and ... m)1Æcm)1at 388 nm).Thermal denaturation studies The thermal denaturation of bsSHMT and the E53QbsSHMT in the absence and presence of ligands, suchas l-Ser and Gly were carried out by measuring the...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... overlapaffecting the corresponding signals. Correlations were calculated bymeans of MATHEMATICA 5.2 software, using the relaxation datasetgiven in supplementary Table S2. Relaxation data obtained ... resultsobtained with the same package. The uncertainties in spec-tral density functions were calculated according to standarderror propagation equations using mathematica 5.2 soft-ware.MD simulationsMD ... N-terminal and C-terminal residues(Arg1–Leu7 and Ala60–Gln67), together with thosefrom Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major cluster,towards the...
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Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... [15,18]. The relative timing of steps involved in dimer formation and a ppearance of thermostabilization byphosphate has been examined. The role of the cofactor5¢-phosphate group in the induction of ... taken after 20, 40 and 60 m in of incubation. The rates were calculated from linear plots of [Pi]released against the reaction time. W hen phosphate was the activator oxyanion, initial rates ... served as the reporter of formation of the apo-enzyme under these conditions. S emi-logarithmic plots of the fraction of remaining active Cc StP against time werelinear, suggesting that inactivation...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

... with EGF and PMAtogether on the one hand, and the sum of the ERK-PPconcentrations after stimulation with EGF and PMAseparately on the other hand, was not constant, butincreased with the EGF ... interactionbetween the MAPK and PKC s ignalling m odule s in a quantitative manner and found that they affect each other in multiple ways. EGF stimulation a nd PKC activation caused a synergistic a ctivation ... biphasic ERK-PPpattern, consisting of an initial peak and a r elaxation to a lowquasi-steady state-phase. Costimulation with the EGF and PKC activator, 4b-phorbol 12-myristate 13-acetate (PMA)resulted...
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Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt

Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt

... PMA-induced AP-1 activation. Theseresults indicate that FADD and caspase-8 are essentialfor FasL-induced AP-1 activation. FasL-induced AP-1 activation requires the catalytic activity of caspase-8We ... causedlittle or no reduction of the FasL-induced AP-1 activa-tion, indicating that the NF-jB activation was notrequired for the FasL-induced AP-1 activation. Thus, the activations of AP-1 and ... FP1mutant inhibited the FasL-induced AP-1 activation. These results indicate that the C-terminal 15 aminoacids of Fas are dispensable, but the DD of Fas isindispensable for its ability to activate...
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