Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... FEBS Proteolytic activation and function of the cytokine Spa ¨ tzle in the innate immune response of a lepidopteran insect, Manduca sexta Chunju An 1 , Haobo Jiang 2 and Michael R. Kanost 1 1 Department ... development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa ¨ tzle in a...

Ngày tải lên: 06/03/2014, 09:22

... (reduction indicated as dashed pattern) by NADH (via FMN-b) induces an instantaneous activation of the Ni-Fe site (removal of the OH – ligand from nickel) enabling the interaction with H 2 .AslongasFMN-ais present, ... total loss of this activity when assayed in the usual way, i.e. after addition of H 2 , a catalytic amount of NADH and the subsequent addition of...

Ngày tải lên: 07/03/2014, 15:20

... practices in language engineering for every new domain. On the other hand, a statistical learning approach needs a large amount of annotated data for model training, which is seldom available in ... expertise in natural language processing and speech recognition, and best practices in language engineering for every new domain. In the past decade many statistic...

Ngày tải lên: 08/03/2014, 02:21

Ngày tải lên: 08/03/2014, 05:21

... into two fragments: the A chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-binding domain. The C and ... triggers a conformational change, leading to insertion of the toxin in the membrane. The C domain is then translocated across the endosomal membrane into the cyt...

Ngày tải lên: 16/02/2014, 09:20

... by: F D ¼ A T À A N =A D À A N Where A N is the absorbance of the protein sample at 30 °C, A D is the absorbance of the sample at 80 °C and A T is the absorbance of the protein sample at different ... was incubated with 10 m M glycine and 500 lM FTHF for 5 min and then dialyzed against buffer D not containing glycine and FTHF. Such an enzyme was once ag...

Ngày tải lên: 18/02/2014, 16:20

... overlap affecting the corresponding signals. Correlations were calculated by means of MATHEMATICA 5.2 software, using the relaxation dataset given in supplementary Table S2. Relaxation data obtained ... results obtained with the same package. The uncertainties in spec- tral density functions were calculated according to standard error propagation equations using mathematica 5.2...

Ngày tải lên: 18/02/2014, 16:20

... [15,18]. The relative timing of steps involved in dimer formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor 5¢-phosphate group in the induction of ... taken after 20, 40 and 60 m in of incubation. The rates were calculated from linear plots of [P i ] released against the reaction time. W hen phosphate wa...

Ngày tải lên: 19/02/2014, 16:20

... with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased with the EGF ... interaction between the MAPK and PKC s ignalling m odule s in a quantitative manner and found that they affect each other in multiple ways. EGF stimulation a nd PKC...

Ngày tải lên: 19/02/2014, 16:20

... PMA-induced AP-1 activation. These results indicate that FADD and caspase-8 are essential for FasL-induced AP-1 activation. FasL-induced AP-1 activation requires the catalytic activity of caspase-8 We ... caused little or no reduction of the FasL-induced AP-1 activa- tion, indicating that the NF-jB activation was not required for the FasL-induced AP-1 activation. Thus,...

Ngày tải lên: 07/03/2014, 09:20