... mixture was placed in a water bath at 70ºC for 1 min. An absorbance measurement was made at 410 nm against a blank sample. Batch culture The experiment was carried out in a batch culture ... Polyhydroxyalkanoic acids act as a form of carbon and energy reserve (Dawes and Senior, 1973) or as an electron sink (Page and Knosp, 1989). Polyhydroxyalkanoic acids are materials of interest because ... denitrificans (Mothes et al., 2007), Cupriavidus necator DSM 545 (Cavalheiro et al., 2009), Bacillus sonorensis, Halomonas hydrothermalis (Shrivastav et al., 2010), Halomonas sp. KM-1 (Kawata and Aiba,...