Báo cáo khoa học: WFIKKN1 and WFIKKN2 bind growth factors TGFb1, BMP2 and BMP4 but do not inhibit their signalling activity docx

... compilation ª 2010 FEBS WFIKKN1 and WFIKKN2 bind growth factors TGFb1, BMP2 and BMP4 but do not inhibit their signalling activity Gyo ¨ rgy Szla ´ ma, Katalin Konda ´ s, Ma ´ ria Trexler and La ´ szlo ´ Patthy Institute ... 300 400 500 600 700 800 Time (s) F BMP2 BMP4 BMP2 -WFIKKN1 BMP4 -WFIKKN1 BMP2 -WFIKKN2 BMP4 -WFIKKN2 Fig. 3. Effect of...

Ngày tải lên: 06/03/2014, 02:20

... interactions [6], a DNA binding domain [7], a linker domain that participates in DNA binding [8], an SRC homology 2 (SH2) domain that mediates dimeriza- tion and receptor binding [9], and a carboxy-terminal transactivation ... from the DNA binding domain of STAT1 harbors overlapping e xport and import activities. Notably, the import activity is observed only in the native STAT dimer,...

Ngày tải lên: 30/03/2014, 15:20

... b-strand 3Õ (i.e. the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop connecting b-strand and a-helix and contiguous residues on the a-helix and the last part of b-strand ... membrane binding and disruption. Note that basicity and hydrophobicity are not exclusive characteri- stics of this part of the molecule, but only loop 3-derived peptides have...

Ngày tải lên: 20/02/2014, 11:20

... the model of GTP binding at the predicted site (Fig. 1A) at the interface between the GATase domain and the synthase domain [13]. Amino acid residues in the synthase domain and residues of lid ... kinetics, the R359M, R359P and G360P enzymes were altered in both GTP binding and activation. The R359M enzyme displayed about fourfold and 10-fold decreases in k cat1 and k cat2 , respe...

Ngày tải lên: 23/03/2014, 13:20

... subunit RII binding domain, the central domain (CD) and the microtubule-binding domain (Fig. 3A). To deter- mine the v-KIND-binding region in MAP2, we first divided MAP2 into five regions (Fig. 3A) and ... regulatory subunit RII binding domain (RII), the central domain (CD)1-3 and the microtubule- binding domain (MBD)] and corresponding GST fusion proteins were generated. (B), Pull-down...

Ngày tải lên: 14/02/2014, 19:20

... to caspase fragments and inhibit their peptide-specific activity, other proteolytic activity still occurs [15,51]. Moreover, cleavage is not necessary to activate caspase-8 and probably its close ... apoptosis, and its inhibitor ICAD. Nature 391, 43–50. 18 Hanus J, Kalinowska-Herok M & Widlak P (2008) The major apoptotic endonuclease DFF40 ⁄ CAD is a deoxyribose-specific and...

Ngày tải lên: 14/02/2014, 22:20

... helix–span–helix DNA-binding and dimerization domain at their C-terminus, and a less conserved proline-rich and glutamine-rich transactiva- tion domain at their N-terminus [8–10]. Most isoforms also ... N-terminal trans- activation domain that is important for their role as transcriptional activators [9]. The AP-2 factors form homodimers and heterodimers for their transcript...

Ngày tải lên: 16/02/2014, 09:20

... disorders [6]. With this method, it is possible to mutate the mouse genome randomly on a large scale, and then isolate and identify the mutated gene. Several other genes have been identified by ... between RMI1 and energy homeostasis as well as the contribution of RMI1 to the regulation of E2F expression, which is a well-documented cell cycle- related molecule. Results In vivo phenotype-...

Ngày tải lên: 16/02/2014, 09:20

... miR-23a promotes growth activity of MGC803 cells. MGC803 cells were transfected with miR-23a ASO or pri-23a vector, and cell growth activity was detected through the MTT (A) and colony formation ... slows PC-3 and LNCaP cell growth, demonstrating its antiproliferative activity [20]. Another study has demonstrated the induction of antiapoptotic regulators by IL6 ⁄ IL6R in both...

Ngày tải lên: 18/02/2014, 04:20

... low-affinity HSE and a binding site for the general stress response transcription factors Msn2 and Msn4 (Msn2 ⁄ 4). However, HSF and Msn2 ⁄ 4 are not preloaded on this promoter. Heat-induced binding of ... protein exhibits a reduced ability to trimerize and is unable to bind to discontinuous gap- and step-type HSEs, but binding to the continuous 3P-type HSE is not affected...

Ngày tải lên: 18/02/2014, 04:20