Báo cáo khoa học: Serine-arginine protein kinases: a small protein kinase family with a large cellular presence potx
Tài liệu Báo cáo khoa học: Purified RPE65 shows isomerohydrolase activity after reassociation with a phospholipid membrane pdf
... Research Resources, a research award from JDRF, a grant from ADA, and a research grant from OCAST HR07-067. References 1 Baylor D (1996) How photons start vision. Proc Natl Acad Sci USA 93, 560–565. 2 ... 5414–5418. 36 Bok D, Ruiz A, Yaron O, Jahng WJ, Ray A, Xue L & Rando RR (2003) Purification and characterization of a transmembrane domain-deleted form of lecithin retinol acylt...
Ngày tải lên: 18/02/2014, 08:20
Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
... radiation facilities and we would like to thank Dr P. Carpentier for assistance in using beamline BM3 0a. References 1 Katayama Y, Hiraishi A & Kuraishi H (1995) Para- coccus thiocyanatus a ... sulfate. X-ray diffraction data of the wt and M100K crystals were collected on an in-house beam using a MAR345 Image Plate detector. The crystals were mounted in a capil- lary and datasets...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx
... RCL, with attached protease, as an additional strand into b-sheet A of the inhibitor, causes a dramatic conformational change in the serpin [9]. Recent X-ray crystallographic diffraction analysis ... vivo clearance of the T–AT complex in rats Thrombin, native AT, and elastase-cleaved AT were labeled with 125 I (Amersham Pharmacia AB, Uppsala, Sweden) using the chloramine T method, acc...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Human mesotrypsin exhibits restricted S1¢ subsite specificity with a strong preference for small polar side chains docx
... wild-type a1 AT in a manner that was comparable with inhibition of cationic and anionic trypsins, demonstrating that Arg198 is the critical determinant of resistance against a1 AT (Fig. 2A, B). Figure 2A ... Mesotrypsin associates with a1 AT Pittsburgh with an SI of 2, and the calculated k a is 1.1 · 10 6 M )1 Æs )1 . Using purified pan- creatic cationic and anionic trypsins and...
Ngày tải lên: 30/03/2014, 10:20
Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG AGCCTTACAAGAAGGGTCCAAAGA-3¢). The PCR product was purified, treated with T4 exonuclease to create vector-compatible overhangs and annealed to a prepared ... chitinolytic machineries, such as in the lactic acid bacterium (LAB) Lactococcus lactis ssp lactis IL1403. LABs are Gram-positive, facultatively, anaerobic,...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... Healthcare). Caspase 9 activity assay Caspase 9 activity was examined according to the instruc- tion manual of the Caspase 9 ⁄ Mch6 Fluorometric Protease Assay kit (Medical and Biological Laboratories ... The Bcl-2 protein family: arbiters of cell survival. Science 281, 1322–1326. 38 Tsukahara T, Kannagi M, Ohashi T, Kato H, Arai M, Nunez G, Iwanaga Y, Yamamoto N, Ohtani K, Nakamura M et...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... antibodies were as follows: anti-porin from Calbiochem, anti-HA from Covance BabCo, anti-mouse and anti-rabbit secondary antibodies from Bio-Rad Laboratories and Amersham Pharmacia Biotech, respectively. Antibodies ... S-HA. The blots were probed with antisera against HA, with anti-porin, and with two other antisera against complex I proteins (MWFE and PSST). (B) BN/PAGE. Top panel: his...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... short-lived transient protein protein interactions by intermolecular nuclear paramag- netic relaxation: plastocyanin from Anabaena variabilis. J Am Chem Soc 125, 6858–6859. Thermodynamic parameters ... thermodynamic parameters for the haems calculated from the NMR data are relative, it could be argued that all haem potentials had been modified to a similar degree but to an unknown extent...
Ngày tải lên: 19/02/2014, 17:20
Báo cáo khoa học: The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response pptx
... Enhanced Ras maturation by heat stress was associated with a heightened activation of extra- cellular signal-regulated kinase (ERK), a key mediator of both mitogenic and stress signaling pathways, ... heat-induced plasma membrane fluidization, are indeed capable of activating HSP formation even at the growth tempera- ture, without causing measurable protein denaturation. We also de...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 2004 hydrolases (Fig. 3). These enzymes share a functional catalytic triad made of a catalytic nucleophile serine, associated to a proton c arrier histidine and a c harge r elaying aspartic (or glutamic) ... and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family Ste ´ phane Canaan 1 , Damien Ma...
Ngày tải lên: 07/03/2014, 16:20