Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

... Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin Alessandra di Masi 1 , Francesca Gullotta 2,3 , Alessandro ... indicates that the redox and the (un)ligated state of the heme Fe atom allosterically affects ibuprofen binding to (t) HSA–heme. l Values of K 2 a...

Ngày tải lên: 06/03/2014, 01:20

... cal- cium displaces the dye bound to calcium -binding sites of the protein. Other proteins of this superfamily, namely c-crystallin [20] and AIM1-g1 [15] also induce the J band of the dye indicating ... 492 and 547 nm (Fig. 3). The enhanced luminescence of terbium in the presence of these crys- tallins indicates that Tyr and Trp residues are in the vicinity...

Ngày tải lên: 18/02/2014, 16:20

... the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly decreased the cytotoxic potential of the molecule. There is now little room ... residues at the N2 position in double- stranded DNA [11]. The covalent binding to DNA is apparently responsible for the cytotoxic action [12] and the ca...

Ngày tải lên: 20/02/2014, 11:20

... screening of new compounds. Results and Discussion Competitive fluorescence experiments to determine the binding of natural substrates to the donor and the acceptor sites of human UCK The ATP -binding ... 6. Model of the acceptor -binding site in the closed form of human UCK. (A) Superposition of the acceptor site with bound CMP (blue) and cidofovi...

Ngày tải lên: 07/03/2014, 09:20

... digested with EcoRI and SalI, and inserted into the p-GBK vector (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the SalIandBamHI sites in the vector. The DNA sequences of all fusion plasmids ... as binding to other proteins at the inner nuclear membrane. Two of emerin’s known binding partners are lamin A and barrier -to- autointegration factor (BAF) [4,5]....

Ngày tải lên: 23/03/2014, 12:20

... bigrams, and trigrams 1 , using the on- topic document collection as the relevant set and the off-topic document collection as the irrelevant set. Figure 1 shows the top 5 concepts with their ... score in the upper range of 2 to 3 and all others had scores lower than 2.5. Some of the systems (including B2) fell into the range of 1 to 2 meaning some or ha...

Ngày tải lên: 17/03/2014, 08:20

... oligoamides consisting of meta-substituted benzene rings linked by glycine residues. The amide O and H atoms act as H-bond donor and acceptor sites. Various arrangements of the amide O and H atoms lead to Ôhydrogen ... 0.10) and the 1 : 1 mixture (R f ¼ 0.96) clearly indicated the formation and high stability of the duplex. Further NMR studies showed significant do...

Ngày tải lên: 23/03/2014, 12:20

... arsenic atoms in FlAsH enables it to bind with high affinity and specificity to the TC motif introduced into a variety of different proteins [15]. The binding of FlAsH reagent and also the quan- tum ... quantify the amount of nonconverted TC-tagged PrP by measuring the fluo- rescence after the addition of FlAsH. The total initial amount of TC-tagged PrP in the...

Ngày tải lên: 06/03/2014, 11:20

... RC-101 doc- kings to dimer and comparative monomer macromolecules were prepared using autodocktools (ADT) and accompa- nying scripts, and then run with autodock 3.0 and auto- grid 3.0 [28,29]. The ... near the a-helix to which they were docked. The RC-101 mole- cules docked to HR1 are much more dispersed than the RC-101 molecules docked to HR2. The color of each...

Ngày tải lên: 07/03/2014, 05:20

... both covalent bonds of FAD to the polypeptide chain of ChitO are required for correct positioning of the flavin to facilitate efficient catalysis. The double anchoring of FAD allows the protein to evolve ... stability, ensure cofactor binding, and or induce a relatively high redox potential of the cofactor. Although the experimental data may show that a cova- lent...

Ngày tải lên: 16/03/2014, 01:20