Báo cáo khoa học: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions pptx

... application of SH2 domain binding peptides, which results in blocking of the binding of the SH2 domain to the substrate and thereby preventing interaction of the substrate with the kinase domain. For ... Y527F D1)250 N-terminal, SH3, SH2, kinase domain and C-terminus Deletion of N-terminal, SH3 and SH2 domain of kinase- dead n -Src B. R. Groveman...

Ngày tải lên: 06/03/2014, 01:20

... embryo- genesis and synthesis of in ammatory cytokines [43,52] and is essential for S100A8 induction in macrophages [27]. Inhibitors of the MEK and p38 pathways both partially abrogated FGF-2 ⁄ heparin- and ... (1 : 200, in saponin–BSA) for 1 h in the dark at 25 °C, rinsed in NaCl ⁄ P i (3 · 5 min) and nuclei stained with DAPI (0.3 nm in NaCl ⁄ P i , 10 min in...

Ngày tải lên: 19/02/2014, 18:20

... understanding of system capa- bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the ... and pen input, includ- ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of mov...

Ngày tải lên: 20/02/2014, 12:20

... apoptosis [73]. The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits to the chicken AP-2. The role of AP-2a was further studied in zebrafish. It ... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen- tiation stages during chondrogenesis and the involvement of transcription factors (he...

Ngày tải lên: 16/02/2014, 09:20

... per monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less in uence (Fig. 5A). To ensure that the estimation of the ... located, and are not found at the ATP-binding site or the subunit–subunit interface of the Rad51 filament. Therefore, the Rad51 L1 and L2 loops may also be...

Ngày tải lên: 19/02/2014, 06:20

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the HSF2 ... neurogenesis and neuronal migration in different parts of the mouse brain. HSF1 and HSF2 might interact in the develop- ing mouse brain in a stage- and tis...

Ngày tải lên: 18/02/2014, 04:20

... role in maintaining SNase tertiary structure. Our CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein. However, in comparison with the wild-type ... located in the C-terminal loop of SNase plays an important role in protein stability and conforma- tional integrity. During protein unfolding ⁄ refolding,...

Ngày tải lên: 20/02/2014, 01:20

... raised against the hemocyanin fraction show staining of the two 75 kDa bands in Western blot, but do not recognize any other proteins of the hemolymph. Although we expected that each of these bands ... observed in SDS/PAGE and in 2D PAGE (Figs 1 and 2; Table 1). The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major s...

Ngày tải lên: 20/02/2014, 11:20

... headword of the node. In the following, the units of PIQ are bits. Conclusion Among the feature types in the same structural position of the parsing tree, the predictive information quantity of lexical ... sequence containing the leaf nodes of the partial parsing tree rooted by the current node, representing the final objectives to be derived from the c...

Ngày tải lên: 20/02/2014, 18:20

... arginines called the arginine fingers. The roles of arginine fingers have been explored in several AAA+ proteins [3–8]. The arginine finger is typically located in the subunit interface, and interacts ... conserved WalkerA and WalkerB motifs, and two arginines (AAA-1) or one arginine (AAA-2). Here, we investigated the roles of these arginines (Arg322, Arg323, and Ar...

Ngày tải lên: 06/03/2014, 00:20