Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx
Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... in actin filament assembly in the actin patch. Binding of the LBD to Las17p may also stimulate Las17p-dependent activation of the Arp2 ⁄ 3 complex. De novo actin filament assembly is critical for ... importance of WASP–WIP interaction in mammalian cells is sup- ported by the observation that the vast majority of the disease-causing missense mutations in human WASP map to t...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx
... which the genes encoding the bifunctional enzymes chorismate mutase–prephenate dehydratase and chorismate mutase–prephenate dehydratase were deleted. Monofunctional versions of the dehydratase and ... better anticipate the natural evolution of bacterial antibiotic resistance. Hydrolysis of aztreonam requires a change in the substrate specificity of TEM-1 b-lactamase. The...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: "Using an Annotated Corpus as a Stochastic Grammar" ppt
... probabilities to these rules such that the probability of the parse of a equals 1/3, and the probability of the parse of ab equals 4/9. The parse of a can exhaustively be generated by applying ... We can estimate a probability as accurately as we want by choosing Nas large as we want, since according to the Strong Law of Large Numbers the estimate...
Ngày tải lên: 22/02/2014, 10:20
Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... local Src activation triggered the long-range propa- gation of active Src wave into the distal cell areas away from the site of mechanical stimulation [50]. The mecha- nism of this wave propagation ... structure of the Abl/Arg and Tec kinase families (in particular, of the c-Abl kinase) resembles the SFK structures. The c-Abl kinase possesses three domains (SH2, SH...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... raised against KLK11 was the same size as that of the translational product by isoform 2 mRNA (Fig. 4B). The product by isoform 1 mRNA was smaller than the isoform 2 product. The multiple bands may ... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT GCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCC GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢ (Fig. 2A) . Luci...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... 4¢-phosphopantothenate is not commercially available, it was synthesized enzymatically in situ by adding pantothenate kinase, His-CoaA, D -pantothenate and ATP to the His-CoaB assay mixtures [5]. Therefore, 0.9 ... with 4¢-phosphopantothenoyl-CMP. Copurification of the 4¢-phosphopantothenoyl-CMP intermediate Taking into account that residues Arg206, Asn210 and Ala276 are crucial for the...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... CTCTTTGATGACAGAAGTGCCT-3¢ Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 365 5¢-450 AAAAATGTTGCTGCCCTG-3¢ Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 488 5¢-1054 CCTTTGAGCACATTTCGGCAA-3¢ P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ ... 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 289 5¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢ b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 665 5¢-3222 AGCCATGCCAAATGTCTCAT-3¢ Fig. 1. Dose...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx
... release, the E-amino groups of lysines in MT2 were carbamoylated with potassium cyanate and the modified protein was assayed for zinc release as described above. Acetaldehyde releases almost the ... to a final concentration of 10 mgÆmL )1 , and solid potassium cyanate was added to a final concentration of 1 m. The reaction mixture was incubated at 37 °C for 24 h. Excess potas...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... and the reactivase eluted separately at their respective retention times. In contrast, a peak of the free reactivase decreased and a new peak appeared in the presence of ADP. The latter peak was ... the enzymeÆreactivase complex was formed in small amounts from apoenzyme and the reactivase and not at all from the enzymeÆCN-Cbl complex and the reactivase (data not s...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
... exclusion, whereas phosphorylated EIIA activates adenylate cyclase (CyaA) and leads to an increase in the intra- cellular cyclic AMP (cAMP) level [1]. Mathematical models of catabolite repression ... the concentration (mass of a compound based on a certain volume, or mass of a compound based on the entire biomass as usual in bio- engineering) is the focus of the mode...
Ngày tải lên: 18/02/2014, 13:20