Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx

Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx

... shows the same analysis for the hits between the N- and C-terminal halves and vice versa within the OPA family, the AAT family, the ST3 families and the DAACS1 family. In case of the AAT and DAACS1families, ... the sequences in the database is about 60%.Hits between the N- and C-terminal halvesEach of the sequences in the domain database wasused as query in a blast search against all domains in the ... the membrane; the N terminus of the N-terminal domainis extracellular, while the N terminus of the C-terminaldomain is intracellular. In the [st326]2HCT family, TMS I is not part of the two-domain structure...

Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx

... core in this family [ 3–6 ]. To iden-tify the catalytic residues involved in the PGDS and PGFS activity catalyzed by AKR1B1, we introducedsite-directed mutagenesis into the tetrad, generating the D43N, ... b-barrel, with the nicotinamide ring projectingdown into the center of the barrel and pyrophosphatestraddling the barrel lip [7]. Kubiseski et al. [8] haveestablished that the enzyme follows ... pros-taglandin. Adv Prostaglandin Thromboxane Leukot Res15, 36 5–3 67.18 Watanabe K, Yoshida R, Shimizu T & Hayaishi O(1985) Enzymatic formation of prostaglandin F2a from prostaglandin H2and...

Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

... different in comparison with thoseof vertebrate trypsins, and also they are important forinhibitor binding [8]. The phenylalanine and Ile resi-dues in crayﬁsh Loop37 interact with the C-terminalsegment ... (216 and 226, chymotrypsin numbering) in bovine and all other crustacean trypsins; (B) predicteddisulﬁde bridges in lobster trypsins; (C)calcium-binding site conﬁguration in lobstertrypsins; ... different trypsins in the digestive gland of the spiny lobster Panulirus argus. EF1-a was used as the housekeepinggene. The same results were obtained when using b -actin as the housekeeping gene...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... differ-ent intensities of the R bands). To eliminate thesedifferences, the effects of DNA cis-platination on the protein–DNA interactions were always normalizedwith the intensity of the retarded band ... accommodate the cisplatin IACs. The higher the probability of formation of the cisplatinIACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the ... the intensity of the R53band resulting from p53 binding to unmodiﬁed DNA was taken as 1.0, and the intensities of bands correspond-ing to p53 binding to the same but cisplatin-treated substrate...

Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human aB-crystallin docx

... containing aB-crystallin induced and not induced with IPTG.Lane 2–5 show aB-cr ystallin uninduced at the 0, 3 and 6 h time points.Lane 7 contains cell homogenates of aB-crystallin n ot induced. ... expression is increased in response to stress, itprotects against protein a ggregation in v itro, and it contains the core Ôa-crystallinÕ domain found in all sHsps. In this studywe characterized the ... by ATP [23]. In separatereports, ATP increased the refolding of xylose reductase bytotal a-crystallin [34], increased the binding of a-crystallin tolens membranes, and inhibited the chaperone...

Báo cáo khoa học: Heme binding to the second, lower-afﬁnity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization potx

... higherafﬁnity binding of ferric heme.Heme binding to proteins is commonly pH-depen-dent [2 6–2 8] and thus heme binding in both the oxi-dized and reduced states was investigated at higherpH. In the oxidized ... effect on the UV-visible or EPR spectra,clearly indicating that these residues are not directly involved in heme binding. Furthermore, low-spin hemebinding to IrrRlalready lacking the HxH motif ... affects the second hemesite, resulting in a loss of low-spin binding and anincrease in high-spin heme (Fig. 1C, D).Taken together, the UV-visible and EPR data indi-cate that His46 is a key ligand...

Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt

... ofthree domains. The N-terminal domain is very similar in sequence and size to IscU, the central domain is similar to aprotein carrying a [2Fe2S] cluster known as Bfd and the C-terminal domain is ... cluster [12].Other proteins which have been shown to be involved in FeS cluster biosynthesis are the two chaperones HscA and HscB. There are indications that HscA/B interact with IscU and it has ... is mobilized from cysteine by the action of the enzyme IscS (or in the case of nitrogenase NifS), a cysteinedesulfurase [4,5]. IscS (NifS) interacts with the iron-bindingprotein IscU (NifU)...

Báo cáo khoa học: Heteromer formation of a long-chain prenyl diphosphate synthase from ﬁssion yeast Dps1 and budding yeast pptx

... ProteinStandard were obtained from Invitrogen (Osaka, Japan).Strains and plasmids The strains and plasmids used in this study are shown in Table 1. The E. coli strain DH5a was used in the generalconstruction ... with the Coq1 protein and the pHADPS1 plasmidthat produces the HA-fused Dps1 protein were introducedinto Sc. pombe strain LA1. Crude proteins extracted from the transformants and puriﬁed proteins ... according to the manufacturer’s instructions. The NativeMark Unstained Protein Standard deviation from the NativePAGE NOVEX Bis-Tris Gel System containseight proteins ranging in size from 20...

Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx

... 2011)doi:10.1111/j.1742-4658.2011.08043.x Starch- binding domains are noncatalytic carbohydrate-binding modulesthat mediate binding to granular starch. The starch- binding domains from the carbohydrate-binding module family 45 (CBM45, ... 1185 Starch- binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism Mikkel A. Glaring1,2, Martin J. Baumann1, Maher ... (2010) The starch- binding capacityof the noncatalytic SBD2 region and the interactionbetween the N- and C-terminal domains are involved in the modulation of the activity of starch synthase IIIfrom...

Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase potx

... This indicates apredominant in uence of the catalytic domain onoligomerization.Role of the C-terminal cysteineMutation of the cysteine located at )4 from the C-ter-minus to a serine in the ... Aa and Ab, as well as for Ba and Bb, indicat-ing a predominant in uence of the catalytic domains. However, addition ofa cysteine within the aromatic-rich segment of the t peptides modiﬁed the oligomeric ... [17]. However, the catalytic domains are also involved in quaternaryinteractions that certainly participate in the formation and stability of these oligomers. In particular, the tet-ramers...

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