Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc

... conserved and unique components of the Cag- T4SS, together with potential implications for the current understanding of its mode of action. The cagPAI and the Cag type IV secretion apparatus Gene ... FEBS MINIREVIEW Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus Wolfgang Fischer Max...

Ngày tải lên: 06/03/2014, 00:21

... proteins in the T4SS apparatus is not clear, although several of them are essential for the secretion of CagA or the induction of interleukin-8 [23]. The NTPases battery of the Cag- T4SS The Cag- T4SS ... picture of the architecture and func- tion of the Cag- encoded T4SS can be derived. Architecture of T4SS The T4SS apparatus generally consists of...

Ngày tải lên: 06/03/2014, 00:21

... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were ... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from the genomic DNA of the corre...

Ngày tải lên: 18/02/2014, 08:20

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... responsible for the LOX activity in immature hazelnut seed. In order to correlate LOX activity and the production of C 6 and C 9 aldehydes deriving from the ac...

Ngày tải lên: 21/02/2014, 00:20

... also positively affected the activity and stability of the enzyme [40]. The effect of copper on enzyme stability may be related to the inhibitory effect of the metal on the activity of an extracellular ... T-vector and sequenced as above. The full- length cDNA of lac1 was then generated by 3¢-RACE using the primer 5¢-TCTCAACCGTCGACAGCAG TGTTCGTG-3¢ designed from...

Ngày tải lên: 07/03/2014, 15:20

... elucidate the role of the positively charged residue Arg78 and the effect of the reduction of cationicity of MBP in the triggering of multiple sclerosis. Flexible docking calculatio ns of the MBP(74–85) epitope ... ey characteristic of the structure of the native epitope in Me 2 SO is the presence of a large number of electrostatic interactions, especia...

Ngày tải lên: 07/03/2014, 16:20

... is the detection of the modification, which involves the detection of a molecular mass change of 1 Da in a relatively large protein substrate (20–45 kDa). Another challenge is the development of ... understanding of the structural and biochemical basis of the effects of these toxins on vari- ous host signaling pathways have provided interesting and sometimes sur...

Ngày tải lên: 22/03/2014, 15:21

... concentrations of Ca 2+ , using PC as substrate, at pH 9.5 and at 37 °C in the presence of 1 m M NaTDC. The star indicates the phospholipase activity measured in the absence of CaCl 2 and in the presence of ... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA 2 activity, the rate of hydrolysis of PC by ChPLA 2 in...

Ngày tải lên: 30/03/2014, 01:20

... thermophilus and its close relatives [16,17]; in these organisms, the heteropentameric POR and OGOR function as the key components of the reductive TCA cycle and catalyze the anabolic reductive carboxylation of ... intermediate in both the oxidative and reductive reactions. The HE-TPP radical was formed only after the reduction of the iron-sulfur clus- ters of...

Ngày tải lên: 16/02/2014, 09:20

... the numbering of the amino- acid sequences of immunoglobulins and the definition of their CDR loops, as described from 1997 [13]. The number- ing and definition of the L-CDR3 and H-CDR3 loops of mAb4E11 ... segment, and the V H -D, D-J H , and V L -J L junctions [17]. They are located at the center of the antibody combining site [18,19] and provide the...

Ngày tải lên: 19/02/2014, 07:20