Báo cáo khoa học: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus pptx

... investigated in detail the roles of these three arginines in TClpB. By combining an arginine mutation with the WalkerA mutation in the other AAA+ module, we tested the roles of the arginines in ATP binding ... the ATP-induced motion of the middle domain. Results The conserved arginines are not involved in nucleotide binding To examine the roles of conserved...

Ngày tải lên: 06/03/2014, 00:20

... 40-fold increase in cGMP levels during a 30-min incubation (Fig. 2); indeed a doubling of cGMP levels could be observed within 2 min of application of hormone (results not shown). For YO taken from premoult ... suggested. Levels of MIH and CHH in the sinus gland during the moult cycle As individual SG from single crabs always contained almost identical profiles and quantities...

Ngày tải lên: 21/02/2014, 00:20

... escalating prevalence of obesity is one of the most pressing health concerns of the modern era, yet existing medicines to combat this global pandemic are disappointingly limited in terms of safety ... suggesting that these cyclin-dependent kinase inhibitors are important in the regulation of adipocyte number. The S-phase kinase-associated protein (Skp)1–Cullin- F-box protein (SC...

Ngày tải lên: 06/03/2014, 01:20

... pain (stimulating scratch- ing) and hemostasis (repairing the wound and involv- ing coagulation), as well as innate, adaptive immune Keywords in ammatory; receptor binding domain; sepsis; serpin; ... 3235 reactions. In response, ticks have evolved a battery of countermeasures [3–7], mainly involving salivary pro- teins, several of which are induced during the feeding process and inject...

Ngày tải lên: 07/03/2014, 01:20

... homeostasis in the air- way mucosa is maintained in order to prevent respirat- ory infections as well as allergic manifestations. However, in an increasing proportion of the popula- tion in industrialized ... 2; house dust mite; protein labelling; selenocysteine Correspondence G. Gafvelin, Karolinska Institutet, Department of Medicine, Clin. Immunology and Allergy Unit, Karolinska...

Ngày tải lên: 07/03/2014, 21:20

... success in parsing definitions with a minimum of incorrect or improbable parses, its success in identifying relational triples, and its speed. Input to the parser was a set of 8,832 definition ... definition grammar. Tagging and head finding were performed on a sample of 2157 noun definition texts, covering the nouns from a through anode. 170 were flagged as ambiguous; o...

Ngày tải lên: 08/03/2014, 18:20

... –Ca 2+ , 15 min in incubation medium; Ca 2+ , 15 min in incubation medium plus 3.5 l M Ca 2+ ;H⁄ R, 10 min hypoxia followed by 5 min reoxy- genation; CSA, 2 l M cyclosporin A. A volume of 2 mL of the incu- bation ... be involved in the patho- genesis of neurodegenerative processes such as tissue infarction resulting from transient ischemia in stroke [1,2]. Investigations of...

Ngày tải lên: 16/03/2014, 22:20

... mechanism of this Fluprostenol-mediated increase in PGF 2a produc- tion in adipocytes, at first we investigated the expres- sion of genes involved in the biosynthesis of PGF 2a and FP receptor in Fluprostenol-treated ... MEK inhibi- tor, PD98059; a PLC inhibitor, U73122; a PKC inhibitor, bisindolylmaleimide I; and a general COX inhibitor, indomethacin, or a selective COX-2 inhibi...

Ngày tải lên: 28/03/2014, 22:20

... statistical ma- chine translation. In Proceedings of the 4th LREC, pages 1585–1588, May. Helmut Schmid. 1994. Probabilistic part -of- speech tagging using decision trees. In Proceedings of International ... Methods in Lan- guage Processing. Kristina Toutanova, Dan Klein, Christopher D. Man- ning, and Yoram Singer. 2003. Feature-rich part -of- speech tagging with a cyclic dependency...

Ngày tải lên: 07/03/2014, 22:20

... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen- tiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of ... transcription factor AP-2 involved in DNA binding. J Mol Biol 301, 807–816. 9 Wankhade S, Yu Y, Weinberg J, Tainsky MA & Kan- nan P (2000) Characterization of the activation domai...

Ngày tải lên: 16/02/2014, 09:20