Proteogly cans Methods and Protocols doc

... Hospital Zurich , Zurich , Switzerland PART I PROTEOGLYCANS sdfsdf 3 Françoise Rédini (ed.), Proteoglycans: Methods and Protocols, Methods in Molecular Biology, vol. 836, DOI 10.1007/978-1-61779-498-8_1, ... Liu and Winston Whei-Yang Kao 19 Shedding of Cell Membrane-Bound Proteoglycans . . . . . . . . . . . . . . . . . . . . . 291 Eon Jeong Nam and Pyong Woo Park 20 Modulato...

Ngày tải lên: 05/03/2014, 23:20

... Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina , Chapel Hill , NC , USA 1 Michael A. McGuckin and David J. Thornton (eds.), Mucins: Methods and Protocols, Methods in Molecular ... which format, and click refresh. Tracks are organized by categories: mapping and sequencing tracks, phe- notypes and disease association, gene and gene predict...

Ngày tải lên: 14/03/2014, 22:20

... Toxin Methods and Protocols Edited by Dana Philpott Frank Ebel 8 Karmali Methods for Detection of STEC in Humans 9 9 From: Methods in Molecular Medicine, vol. 73: E. coli: Shiga Toxin Methods and ... coli O157:H7 and Other Shiga Toxin- Producing E. coli Strains (Kaper, J. B. and O’Brien, A. D., eds.), ASM, Washing- ton, DC, pp. 183–194. Humana Press E. coli Shiga Toxin Met...

Ngày tải lên: 23/03/2014, 07:20

... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...

Ngày tải lên: 08/10/2012, 10:25

... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The M...

Ngày tải lên: 08/10/2012, 10:25

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager....

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolu...

Ngày tải lên: 08/10/2012, 10:25