Báo cáo khoa học: Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L ) ppt
Báo cáo khoa học: Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.) ppt
... Journal 278 (201 1) 3076–3085 ª 2011 The Authors Journal compilation ª 2011 FEBS 3085 Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza ... class A-HSFs, using a novel BiFc fragment, and identification of novel class B-HSF interacting proteins. Eur J Cell Biol 89, 126–132. D. Mittal et al....
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa học: Binding affinity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity pot
... Japan) in low-salt buffer (20 m M Hepes, 20 m M NaCl, 20% glycerol, 1 m M EDTA, 1m M 2-mercaptoethanol, pH 7.9, containing 1 lgÆmL )1 aprotinin, 1 lgÆmL )1 pepstatin and 1 lgÆmL )1 leupeptin) with 3 H-labeled ... (PerkinElmer Life Sciences, Wellesley, MA, USA) using a liquid scintillation counter LSC-1000 (Aloka Co., Ltd, Tokyo, Japan). Ligand binding to the cell-free prepar...
Ngày tải lên: 23/03/2014, 21:20
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... Sequestering of the fibrils into intracellular inclusions or extracellular plaques might actually be beneficial, as it is now believed that soluble oligomers are the cause of the initial insult rather ... protein. Typically an initial injection of 2 lL copper chelate solution was followed by a further 29 injections of 4 lL of Cu(II) into the protein in the sample cell stirred at 300 r.p.m. In...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx
... mutant (no binding activity) and wild-type (100% binding activity) mini-receptors in the mixture would follow the line displayed in the bar graph, suggesting that the wild-type binding site on ... These results can be explained either by a sterically improbable intramolecular binding mechanism or by binding of a multivalent ligand forcing receptors to realign within the immuno...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... full-length PrP was ana- lyzed after deglycosylation. While N-terminal binding antibodies as SAF34 detected only full-length PrP C , C-terminal binding antibodies recognized two bands comprising ... post- translational modification involving removal of an N-terminal signal peptide and C-terminal residues in the polypeptide chain and attachment of a glycosyl- phosphatidylinositol grou...
Ngày tải lên: 19/02/2014, 02:20
Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... on HFE intracellular trafficking, localization and func- tion. Initially this was done in the context of inhibiting cellular glycan production, with tunicamycin treatment altering HFE localization ... glycosylated proteins. As might be expected from the ER-localized and potentially misfolding triple mutant, we observed little or no regulation of transferrin binding in cells express-...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Binding of the volatile general anesthetics halothane and isoflurane to a mammalian b-barrel protein doc
... K d values of 80 ± 10 lm and 100 ± 10 lm, respectively. Halothane and isoflurane binding resulted in an overall stabilization of the folded conformation of the protein by )0 .9 ± 0.1 kcalÆmol )1 . In ... other ligands [23] shown crystallographically to occupy the internal cavity of the protein [24]. Briefly, por- cine odorant binding protein samples (1 lm), containing a fixed...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans potx
... N-acetylglucosamine; GnT, N-acetylglucosaminyltransferase; ICAM, intracellular adhesion molecule; LPS, lipopolysaccharide; LSECtin, liver and lymph node sinusoidal endothelial cell C-type lectin; ... proteins to Lec8 cells was abolished. We then performed inhibition tests using a GlcNAc- binding lectin from Psathyrella velutina (PVL). When PVL (1 mgÆmL )1 ) was pre-incubated with L...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot
... described in the text. Values determined from microcalorimetric data. Temperature (°C) DH (kcalÆmol )1 ) K b · 10 )6 (M )1 ) n DG (kcalÆmol )1 ) TDS (kcalÆmol )1 ) DC p (calÆmol )1 ÆK )1 ) 10 )0 .2 ... change, using DG = DH)TDS. K b (·10 6 M )1 ) DG (kcalÆmol )1 ) DH (kcalÆmol )1 ) TDS (kcalÆmol )1 ) 1.2 ± 0.1 )8 ....
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx
... fertilization, cell proliferation, cell defense, and cell death [1]. Calmodu- lin (CaM), a ubiquitous 17-kDa cytosolic protein, is a major cellular Ca 2+ sensor which rapidly regulates intracellular processes ... domains bind NADPH, FAD, and FMN, and the oxygenase domain con- tains binding sites for heme, tetrahydrobiopterin (H 4 B), and the substrates l- arginine and molecular o...
Ngày tải lên: 16/03/2014, 13:20