Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

... upregulates the expres- sion of the mitochondrial protein uncoupling protein 2 (UCP2), and pre- treatment with vitamin E attenuates upregulation of UCP2. These findings suggest that the MDP-induced reactive ... (20 11) 3054–3064 ª 20 11 The Authors Journal compilation ª 20 11 FEBS Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is...

Ngày tải lên: 05/03/2014, 23:20

... young seedlings and vascular tissues of leaves, such as the midrib of the cotyledon, the hypocotyl, and the root vasculature (Fig. 2E). When the plants were 2 weeks old, the GUS staining in the vascular ... Journal 27 8 (20 11) 22 96 23 06 ª 20 11 The Authors Journal compilation ª 20 11 FEBS 22 97 In order to investigate the detailed expression pat- tern...

Ngày tải lên: 14/02/2014, 18:20

... 3. Distribution and frequency of EGFR mutations occurring in the kinase domain in lung cancer (upper part of the figure) [ 12] and in the extracellular domain in glioblas- toma (lower part of the ... corresponding to domains I and II. In this case, the EGFR tyrosine kinase is acti- vated constitutively without ligand binding, as in the case of EGFR vI. E...

Ngày tải lên: 16/02/2014, 09:20

... genotype-directed therapy in this disease. KRAS mutation as a mechanism of resistance to EGFR-targeted therapy The KRAS protein is localized to the inner surface of the cell membrane. The binding of ligand to EGFR induces ... result in reposition- ing of critical residues surrounding the ATP-binding cleft of the tyrosine kinase domain of the receptor and th...

Ngày tải lên: 16/02/2014, 09:20

... mitogen-activated protein kinase kinase family after being delivered to the cytosol by the cell- binding component of the toxin, protective antigen. The effect of cleaving these mitogen-activated protein kinase ... mutations, K518E and E682G (Fig. 1A). Lys518 is within a patch of amino acids that has previously been implicated in binding MAPKKs [22 ]. Glu6 82 is wit...

Ngày tải lên: 16/02/2014, 09:20

... that includes a DNA-binding domain, a ligand-binding domain and a transactivation domain. The DNA-bind- ing domain is responsible for DNA binding specificity and dimerization, and the ligand-binding ... as E2F family proteins. Whereas MLL1 interacts with E2F2, E2F4 and E2F6, MLL2 interacts with E2F2, E2F3, E2F5 and E2F6 [34]. Similar to E2Fs, the G 1 -phase regulator HCF-...

Ngày tải lên: 16/02/2014, 14:20

... functions of the MLL protein The MLL protein has been identified as the mamma- lian orthologue of the Trithorax protein in inverte- brates [6]. Disruption of this gene in invertebrates and vertebrates ... signature that is further enhanced by the presence of the HoxA9 protein [20 ]. Men1 binds directly to the N-terminus of MLL fusions [22 ] and was ess...

Ngày tải lên: 16/02/2014, 14:20

... quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72, 24 8 25 4. Isomerohydrolase activity of purified RPE65 O. Nikolaeva et al. 3030 FEBS Journal 27 6 (20 09) 3 020 –3030 ª 20 09 ... AU) 025 5101 520 Time (min) 1 .2 0 0.8 0.4 A 320 (1 × 10 2 AU) 0.6 0 0.4 0 .2 A 320 (1 × 10 2 AU) 1 .2 0.8 025 5101 520 Time (min) 0.6 0 0.4 0 .2 A 3...

Ngày tải lên: 18/02/2014, 08:20

... 5-bromo-4-chloro-3-indolyl-b- D-galactopyranoside. 527 2 FEBS Journal 27 5 (20 08) 527 2– 528 5 ª 20 08 The Authors Journal compilation ª 20 08 FEBS simplex virus glycoproteins, gB, glycoprotein H (gH), and glycoprotein ... (2% formaldehyde and 0 .2% glutaradehyde). The cells were then washed with NaCl ⁄ P i and permeabilized with 2mm MgCl 2 , 0.01% deoxycholate and 0. 0...

Ngày tải lên: 18/02/2014, 14:20

... bonding between the imidazole nitrogen of the conserved His and the hydroxyl of the active-site Ser increases the nucleophilicity of the Ser oxygen, facilitating attack of the hydroxy group on the ... condensation of tetraethoxysilane (TEOS) have been determined; the catalytic triad is histidine (His), asparagine (Asn) and serine (Ser) [4, 12] . In the...

Ngày tải lên: 18/02/2014, 16:20