Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc

... 2001 geometry, the set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the ... mobility with respect to the rest of the structure. Further studies of the receptor bound forms of the chimera and other related ligands will be necessary...

Ngày tải lên: 22/02/2014, 07:20

... The MBD of MBD1 contacts the methyl groups of a methylated CpG through a hydrophobic patch formed by the side chains of five residues, V20, R22, Y3 4, R44, and S45 [19]. Although the solution structure ... one-turn helix on the other face. It is thought that the two inner strands of the b-sheet lie within the major groove of the DNA and that a hydrophobic pocke...

Ngày tải lên: 21/02/2014, 00:20

... mecha- nism of transphosphorylation, which implies the A B Fig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-site residues of the 20 conformers of the solu- tion structure ... more economical. At the same time, the active site of HtA, as revealed by the 3D structure, shows a different arrangement to that shown by the classic...

Ngày tải lên: 18/02/2014, 08:20

... )1 topology [47] of the triple-stranded antiparallel b-sheet was Fig. 3. Summary of data used in the determination of the secondary structure of crotamine. (A) Primary sequence of Crt with the sequential ... we find that Gly9, His10, and Cys11 have their counterpart in residues Gly34, Tyr35 and Cys36 in the strand b 2 of AaHII. Noteworthy, the residues Gly9–Cys11 in C...

Ngày tải lên: 20/02/2014, 11:20

... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-affinity peak was the same for the ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, t...

Ngày tải lên: 21/02/2014, 15:20

... 246, 531–544. 20 Lim JH, Yu YG, Han YS, Cho S, Ahn BY, Kim SH & Cho Y (1997) The crystal structure of an Fe-superoxide dismutase from the hyperthermophile Aquifex pyrophi- lus at 1.9 A ˚ resolution: structural ... significantly lower than the metal-containing enzyme. Metal cofactors were incorporated into the enzyme; when the growth medium contained MnSO 4 or FeSO 4 , th...

Ngày tải lên: 14/02/2014, 22:20

... reviews [1,2,10]. S1 The following segments border the S1-specificity pocket of DESC1: Asp189–Gln192 (the basement of the pocket), Ser214–Gly219 (the entrance frame), Lys224– Tyr228 (the back of the pocket) and the ... display nicely the interaction of the S1 site, but do not interact with the S2 site of these proteinases. By contrast, the aspartates in positions...

Ngày tải lên: 19/02/2014, 00:20

... anthracis. The protein exhibits deacetylase activity with the N-acetyl moiety of the N-acetylglucosamine and the diacetylchitobiose and triacetylchitotriose. However, neither the specific substrate of the ... the 1-d-myo-inosityl-2-acetamido-2-deoxy-a-d-glucopyrano- side [6]. Independent of their specific substrates, all members of this family deacetylate the N-acetyl gr...

Ngày tải lên: 19/02/2014, 00:20

... used. RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... Thus, it is the cleavage of the interdomain loop and not of the autolysis or other loops that determines the half-life of chymotrypsin activity. Keywords: auto...

Ngày tải lên: 22/02/2014, 07:20

... perturbation of the axial symmetry of the tensor, whereas axial symmetry, i.e. g xx % g yy << g zz is typically found for type 2 sites. The grouping of the g-values and the line-shape of the spectrum ... Vlaardingen, the Netherlands; 2 University of Groningen, Laboratory of Biophysical Chemistry, Groningen, the Netherlands; 3 Department of Molecular Physics...

Ngày tải lên: 22/02/2014, 07:20