Tài liệu Báo cáo Y học: Use of site-specific recombination as a probe of nucleoprotein complex formation in chromatin Micha Schwikardi and Peter Droge ¨ potx

... Use of site-specific recombination as a probe of nucleoprotein complex formation in chromatin Micha Schwikardi and Peter Dro¨ge Institute of Genetics, University of Cologne, Germany DNA transactions ... targets spanning 114 bp was impaired. This impediment was maintained over at least 72 h and was not affected by the transcriptional status of chromati...

Ngày tải lên: 22/02/2014, 07:20

... [15]. In vitro phosphotransferase assays Pyruvate evolution was measured spectrophotometrically in 96-well microtiter plates at 30 °C, using the coupled assay with L -lactate dehydrogenase and NADH. ... rate of inactivation by bromoacetyl-Glc ( 1a) was 15 times faster than by bromoacetic acid and 2.5 times faster than by the chemically more reactive iodoacetamide, suggesting some sp...

Ngày tải lên: 21/02/2014, 01:21

... 5¢-CCGAATTCTTAGGACCGACCGTTTTGG-3¢ At173sense 5¢-GCGCTCGAAAATCTATTCAAGAACACC-3¢ At173anti 5¢-GTTCTTGAATAGATTTTCGAGCGCACCGATGATAAC-3¢ Bn173sense 5¢-GCCCTAGAAAATCTATTCAAGAACACC-3¢ Bn173anti 5¢-GTTCTTGAATAGATTTTCTAGCGCACCATT-3¢ At399sense ... 5¢-TTTAGAGACACTTGATTTGAGATGCGG3¢ At114V93sense 5¢-CCGCATCTCAAATCAAGT GTCTCTAAA-3¢ At114V95Ianti 5¢-CATGACTTTAGAGATACTAACTTTGAG-3¢ At114V95Isense 5¢-CTCAAAGT...

Ngày tải lên: 21/02/2014, 15:20

... prerequisite for task completion and user satisfaction. Typically, such systems are based on a dialogue strategy that is manually designed by an expert based on knowledge of the system and the domain, and ... assess, because the effectiveness of a strategy depends on many different factors, such as classification/ASR performance, the dialogue domain and task, and, perhaps m...

Ngày tải lên: 20/02/2014, 12:20

... of this signal, as cross-linking of GPVI in the absence of FcR c-chain was unable to promote an increase in phosphorylation of Syk. A transmembrane arginine residue and the cytoplasmic tail of ... of Fura-2 emissions were measured and analyzed using phycoerythrin FWINLAB software. Ratiometric analysis was converted into a concentration of Ca 2+ by measuring R max (...

Ngày tải lên: 22/02/2014, 07:20

... in ammatory bowel dis- ease because 12-LOX activity is induced at active sites of this disease. Because HXA 3 may play an important step underly- ing the pathophysiology of in ammatory diseases, such as ... MINIREVIEW Bacterial-induced hepoxilin A 3 secretion as a pro -in ammatory mediator Beth A. McCormick Department of Pediatric Gastroenterology, Massachusetts General Hospi...

Ngày tải lên: 19/02/2014, 02:20

... heuristic 333 estimate of the remaining cost for completing the translation. The variant which is mostly used is a form of beam-search, where several partial can- didates are maintained in parallel, and candidates for ... been to propose a transformation for an arbitrary phrase- based SMT decoding instance into a TSP instance. While certain similarities of SMT decoding and T...

Ngày tải lên: 20/02/2014, 07:20

... process, and K d at 0.1 MPa. Pressure inactivation of enolase was measured by subjecting dilute solutions of enolase to pressure for varying times, returning to 0.1 MPa, and immediately assaying the sample ... phospho- fructokinase [22], hexokinase [23], lactate dehydrogenase [24], and creatine kinase [25], have been examined by using pressure. I n these examples, and others, pres...

Ngày tải lên: 19/02/2014, 16:20

... Use of Mutual Information Based Character Clusters in Dictionary-less Morphological Analysis of Japanese Hideki Kashioka, Yasuhiro Kawata, Yumiko Kinjo, Andrew Finch and Ezra W. Black ... in a 26- character alphabet, the domain of possible characters appearing in an average Japanese text is a set involving tens of thousands of characters, 658 2.1 Character S...

Ngày tải lên: 20/02/2014, 18:20

... Chile; 2 Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada Escherichia coli phosphoenolpyruvate (PEP) carboxykinase catalyzes the decarboxylation of oxaloacetate and ... binding; conformational changes; P-pyridoxyl fluorescence spectroscopy. Escherichia coli phosphoenolpyruvate carboxykinase [PEP carboxykinase; ATP:oxaloacetate carboxylase (tran...

Ngày tải lên: 21/02/2014, 01:21