Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

... Structural and biochemical characterization of neuronal calretinin domain I II (residues 1 1 00) Comparison to homologous calbindin D 28k domain I II (residues 1 9 3) Małgorzata Palczewska 1 , ... of CR I II and Calb I II are an indication of different organizations of EF-hands within full-length CR and Calb. CR I II and Calb I II may...

Ngày tải lên: 22/02/2014, 07:20

... ligases. Biochemistry 41, 14 299 14 305. 26 Chelvanayagam G, Heringa J & Argos P (19 92) Anat- omy and evolution of proteins displaying the viral cap- sid jellyroll topology. J Mol Biol 228, 220–242. 27 Anand ... 4345 Structural and biochemical characterization of a human adenovirus 2 /12 penton base chimera Chloe Zubieta 1, *, Laurent Blanchoin 2 and Stephen Cusack 1...

Ngày tải lên: 19/02/2014, 06:20

... member of the S100 family, the S100 A12 [10 ]. Hofmann et al. [11 ] proved that S100 A12, and possibly other members of the S100 family, mediates the activation of a novel proinflammatory axis by binding ... the EF-hand superfamily, comprising 16 known members from mammalian species (S100 A1 to S100 A13, S100 B, Calbindin D 9k ,S100P )and two putative additional members identified...

Ngày tải lên: 08/03/2014, 22:20

... paper S103 Y1 12 R207 /19 9 K173 /16 5 G145/D137 D142 /13 4 E1 41/ 133 L75/67 D2 01/ 193 G77/69 G197 /18 9 G145/D137 A B Fig. 2. Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues ... Val143 and Phe194. N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7 (Fig....

Ngày tải lên: 15/02/2014, 01:20

... revised 18 May 2009, accepted 28 May 2009) doi :10 .11 11/ j .17 42-4658.2009.0 712 0.x Nitric oxide synthases belong to a family of dual-flavin enzymes that trans- fer electrons from NAD(P)H to a variety of ... Chapman SK (19 99) Potentiometric analysis of the flavin cofactors of neuronal nitric oxide synthase. Biochemistry 38, 16 413 16 418 . 68 Dunford AJ, Rigby SEJ, Hay S, M...

Ngày tải lên: 18/02/2014, 11:20

... (2008) Correction of pre-steady state KIEs for isotopic impu- rities and the consequences of kinetic isotope fraction- ation. J Phys Chem A 11 2, 13 109 13 115 . 40 Hay S, Pudney CR, Sutcliffe MJ & Scrutton ... Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo- specificity of 7-alpha-hydroxysteroid and 12 -alpha-hy- droxysteroid deh...

Ngày tải lên: 18/02/2014, 11:20

... specificity and efficient coenzyme orientation [99 ,11 5 ,12 1]. The 15 5 16 0 and 2 61 2 68 loops, which accommodate the coenzyme pyrophosphate portion, also confer specificity and the volume of residues ... Jungs YS, Albee L, Golbeck JH & Chitnis PR (19 96) Mutational analysis of photosystem I poly- peptides. Role of PsaD and the lysyl 10 6 residue in the reductase activit...

Ngày tải lên: 18/02/2014, 11:20

... K i C AB B A A B E204 E204 T197 T197 A C F133 F133 T1 31 T1 31 A C D E A K102 D104 K102 D104 D104 K102 C E D Fig. 3. (A) Interactions between subunits A, B and C. (B) Hydrophobic interaction between ... activities of F133N and F133A were only 50 and 20% of that of the wild-type enzyme. Similar to the situation with wild-type UpUMPK, addition of 0.5 and 1 mm GTP resulted...

Ngày tải lên: 18/02/2014, 16:20

... activity that differ between opistoporin 1 and pandinin 1 are the hydrophobicity (pandinin 1 0 .12 14, opistoporin 1 0 .16 52) and the hydrophobic moment, which is nearly double for pandinin 1 (pandinin ... 60 51 6.3 6.3 12 .5 25 3 .1 6.3 6.3 12 .5 B. subtilis IP 5832 50 ND 12 .5 25 12 .5 12 .5 12 .5 25 L. monocytogenes NCTC 11 994 6.3 6.3 12 .5 12 .5 6.3 3 .1 25 25...

Ngày tải lên: 21/02/2014, 15:20

... also WT F63C F63D F63E F63G F63H F63I F63K F63L F63M F63N F63P F63Q F63R F63S F63T F63V F63W F6 3Y WT L92C L92D L92E L92F L92G L92H L92I L92K L92M L92N L92P L92Q L92R L92S L92T L92V L92W L9 2Y WT F150C F150D F150E F150G F150H F150I F150K F150L F150M F150N F150P F150Q F150R F150S F150T F150V F150W F15 0Y 12 0 10 0 80 60 Relative activity (%) 40 20 0 12 0 10 0 80 60 Relative activity ......

Ngày tải lên: 18/02/2014, 08:20