Tài liệu Báo cáo Y học: Binding of gelsolin domain 2 to actin An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin pptx
Tài liệu Báo cáo Y học: Binding of gelsolin domain 2 to actin An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin pptx
... experiments (sequence 11 4 22 5). Two peptides belonging both to the 11 4 22 5 sequence and exposed regions of subdomain 1 were tested (1 12 – 12 5 and 11 9 1 32 peptides). Interaction of the 15 9 19 3 fragment ... m M a 84 10 3 No binding No binding 1 2 mM a 1 12 12 5 No binding 50 mM 4 mM [ 51] 11 9 1 32 2.9 m M 2. 0 mM 12 mM [ 51] 347–365 2 m M Bind...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
... boiled NK1LK2 (nM) Not boiled 2 20 20 0 20 2 20 20 0 20 20 20 20 20 20 20 TMPRSS13 (n M) TMPRSS13 (nM) 50 37 75 (kDa) 50 37 75 10 0 NK1 (n M) Boiled NK1LK2 (nM) Boiled 2 20 20 0 20 2 20 20 0 20 20 ... 20 0 20 20 20 20 20 20 20 TMPRSS13 (n M) TMPRSS13 (nM) NK1 (n M) NK1LK2 (nM) 2 20 20 0 20 2 20 20 0 20 20 20 20 20 20 20 B TMPRSS13 (nM) TM...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... assay conditions [CB2, CB2,4 and CB3 from a1(I), CB 12 , CB8 and CB10 from a1(II)]. (b) A ll peptides able to bind decorin contain a region corresponding to the d and e bands of collagen fibrils (Fig. 2 ). This ... equally to this work. (Received 3 December 20 01, accepted 1 4 January 20 02) Eur. J. Biochem. 26 9, 1 428 14 37 (20 02) Ó FEBS 20 02 number of ionic...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... 4.347 2. 389 1. 978 1. 978 1. 978 3.336 3.336 Asp9 – 4.666 2. 707 2. 543 Thr10 8.386 4 .25 2 4 .15 5 1. 134 Arg 11 8.484 4.574 1. 798 1. 724 1. 629 1. 629 3 .16 0 3 .16 0 Pro 12 4. 327 2. 255 1. 978 1. 978 1. 879 3.7 82 3.5 82 CONH ... – 4.347 2. 397 1. 983 1. 983 1. 983 3.368 3. 317 Asp9 – 4.779 2. 747 2. 558 Thr10 8. 914 4.445 4 .29 7 1 . 21 7 Arg 11 8. 510 4.50...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... °Cfor1h. Thus treated proteins w ere extracted with SDS and then analyzed by SDS/PAGE, followed by CBB staining and autoradiography. Lanes 1 and 4 , GST; lanes 2 and 5, GST–NK; l anes 3 and 6, ... Primary structure analysis, and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. J. Biol. Chem. 26 9, 11 306– 11 311 ....
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... 10 )4 1. 17(3) · 10 )3 7(3) · 10 )7 2. 1 (2) · 10 )6 DAHam 1. 72( 5) · 10 )3 3 .2( 2) · 10 )2 1. 17(3) · 10 )6 1. 90(3) · 10 )3 DAHKam 5.8 (1) · 10 )3 2. 1( 1) · 10 )2 9 .2( 8) · 10 )7 3.0 (1) · 10 )5 BSA 2. 56(7) ... ( )1. 33) 5 52 ()0. 72) 20 6 2. 18 407 (+0.65) 477 (+0.34) 27 1 ( +1. 35) 315 ( +1. 33) 26 1 ( +1. 56) 27 5 ( )2. 50) DAHam a 4...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
... 3 December 20 01, revised 4 April 20 02, accepted 2 May 20 02) Eur. J. Biochem. 26 9, 29 71 29 79 (20 02) Ó FEBS 20 02 doi :10 .10 46/j .14 32- 10 33 .20 02. 029 73.x (Sima ´ ndi, L.I., ed.), pp. 26 9 27 8. Elsevier ... kaempferol, myricetin and galangin (red circles) have g // values ranging from 2. 3 31 to 2. 337, rather small A // parameters (11 .0– 11 .5 mT) and f...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... Calculation of the banding patterns of 17 gels using antibody SAF34 gave an SE of 2. 1 for the di-glycosylated isoform, 1. 0 for the mono-glycosylated band and 1. 6 for the nonglycosylated protein; ... J Biol Chem 28 0, 1 12 4 7 1 12 5 8. Glycotyping of PrP C T. Kuczius et al. 15 02 FEBS Journal 27 4 (20 07) 14 92 15 02 ª 20 07 The Authors Journal compilation ª 20...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... & Costa J (19 91) Dissociation constants of Bronsted acids in D 2 O and H 2 O: studies on polyaza and polyoxa–polyaza macrocycles and a general correlation. Anal Chim Acta 24 5, 27 1 28 2. 49 Pierattelli ... gratefully acknowledged. Financial support was provided by FCT-POCTI, Co- financed by FEDER (POCTI ⁄ 429 02 ⁄ QUI ⁄ 20 01 to CAS, POCTI ⁄ 43435 ⁄ QUI ⁄ 20 01 to...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... f 2 v 2 – 5 .15 0.34 1 . 21 0.66 1. 1 ATP, Mn 2+ 5 .27 0.37 1. 17 0.63 1. 06 PEP, Mg 2+ 5. 52 0.47 1. 10 0.53 1. 12 PEP, Mn 2+ 6 .10 0. 51 1 .14 0.49 1. 06 ATP, oxalate, Mg 2+ 5.75 0.45 1. 00 0.55 1. 16 Ó FEBS 20 02 ... 5¢-phosphate; P-pyridoxyl, phosphopyridoxyl. (Received 16 May 20 02, revised 26 July 20 02, accepted 21 August 20 02) Eur. J. Biochem. 26...
Ngày tải lên: 21/02/2014, 01:21