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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx

Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx

Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx

... Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents Kunio Ohyama1, Bo Yuan1, ... death in the chorion laeve trophoblast layer of human fetal membrane tissues during the late stages of pregnancy, the progression of apoptosis during incubation in vitro, and its suppression by a ... TNFa-induced U937 cell apoptosis by maintaining mitochondrial integrity and function [43], andricin-induced apoptosis of U937 cells by maintaining anintracellular reducing condition by acting...
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Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

... the synthesis of poly(A)catalyzed by yeast poly(A) polymerase. The relativeactivity of diadenosine polyphosphates as effectors of the poly(A) synthesis was assayed as in Fig. 4, using0.05 mM[a-32P]ATP ... on the catalytic properties of yeast poly(A)polymerase and on the intracellular role of dinucleosidepolyphosphates, a family of compounds of increasingmetabolic and regulatory interest [7–11].MATERIALS ... [1]. This novelproperty of E.colipoly(A) polymerase moved us to analyzewhether these compounds were also substrates of eukaryoticyeast poly(A) polymerase. The yeast enzyme is involved in the...
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Tài liệu Báo cáo Y học: Tissue factor pathway inhibitor A possible mechanism of action doc

Tài liệu Báo cáo Y học: Tissue factor pathway inhibitor A possible mechanism of action doc

... al.[8]suggeststhatthis inhibitory complex can be the result of binding of TFPIto the enzyme–product Xa–VIIa–TF complex and this is themain pathway of inhibition of VIIa–TF during ongoingfactor X ... Verification of the hypothesis of the enzyme–product complexinhibition by TFPI. Factor Xa is inhibited by TFPI in the presence or in the absence of VIIa–TF. The concentrations of the reagents are:[Xa] ... depending onplasma state. Therefore elucidation of the details of theTFPI inhibitory mechanism is of great interest.TFPI is a Kunitz-type inhibitor containing three Kunitz-type domains. The...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... theactivity of the enzyme was measured by the dinitrosalicylicacid method.Inhibition of the enzyme activity by glucose, maltose andcyclohexaamyloseFor the study of the enzyme inhibition by glucose, ... notaffected by the inhibitors of the enzymatic activity.Therefore the iodine staining method is well suited forextensive kinetic analyses of purified b-amylases.Stability testsThe heat stability of ... other. Only four of the cysteine residues of the C. sepium b-amylase (Cys83,Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343).On...
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Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx

Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx

... allowchemical characterization of each. CO3-1 was identified astrehalose by TLC as well as by MALDI-TOF MS analysis of the products obtained by permethylation and hydrolysis,andalsoby1H-NMR.Oligosaccharide ... synthesis of trehalose-containing trisaccharide using various glycohydrolases.Carbohydr. Res. 199, 227–234.23. Muramatsu, M. & Nakakkuki, T. (1995) Enzymatic synthesis of novel fructosyl ... role in mycobacteria, perhaps during latency.Keywords: Mycobacteria, oligosaccharides; trehalose.The mycobacterial cell wall is an exceedingly complexnetwork of interacting molecules and includes...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "An ISU Dialogue System Exhibiting Reinforcement Learning of Dialogue Policies: Generic Slot-filling in the TALK In-car System" pot

... actions, for each sub-task type of the in- car system.121An ISU Dialogue System Exhibiting Reinforcement Learning of DialoguePolicies: Generic Slot-filling in the TALK In- car SystemOliver Lemon, Kallirroi ... the system, as well as the pur-poses and future use of the system, and surveysthe research issues involved in its construction.Evaluation of this system (i.e. comparing thebaseline system ... a nd used in the in- car scenarios.3.2 Fragmentary ClarificationsAnother research issue we have been able to explore in con structing this system is the issue of generatingfragmentary clarifications....
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... properties. In this work we took advantage of the specific labelingwith PLP of Lys288 in E. coli PEP carboxykinase to gaininsight into conformational alterations of the protein uponligand binding in ... dynamicalproperties of the local environment of the P-pyridoxylgroup due to changes of the protein conformation induced by the binding of PEP or the ternary combination.Quenching studies of the labeled protein ... crystal coordinates of the E. colienzyme indicate that this residue, located in the C-terminaldomain, is 9.7 A˚from Gly251, which is the closest aminoacid residue of the N-terminal domain,...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... stereoselective synthesis of enantiomericallypure mono- and di-O-hexadecyl-b-D-glucosylglycerol ethers by epoxidation of an allyl b-D-glucopyranoside asymmetricallyinduced by the glucide moiety. Tetrahedron: ... 6-deoxy-6-isothiocyanato-a-D-glucopyranoside (1e), b-D-glucopyranosyl isothiocyanate(3c)andb-D-glucopyranosyl phenyl isothiocyanate (3d).Phosphorylation of EIIGlcprotects, indicating that inacti-vation ... be excluded is thatdephosphorylation and/or catalytic turnover of EIIGlc,rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant cysteine in homologous...
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

... methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with theN-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA. J. ... Kinetically this reaction schememeans that two methyl groups are always introduced, oneslowly by Dnmt3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast. Therefore, this effectcan ... anissue of our work, we present a mechanistic basis for thefunctional cooperation of Dnmt3a and Dnmt1 in de novomethylation of DNA that can only be provided by in vitro experiments. In this model...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... polysaccharides, in particular pectin. Two types of arabinogalactan side chains are present in pectin. Type Iconsists of a chain of b-1,4 linkedD-galactopyranoselinkages, while type II contains a backbone ... CREAbinding sites in the promoter of galA is functional.Hydrolysis of arabinogalactans with a different degree of L-arabinose substitution by GALA results in differentproducts. Arabinogalactans ... is released from soy arabinogalactan, whichincreased upon prolonged incubation. MALDI-TOF MS analysis allowed for a more detailedanalysis of the products formed during the incubations,although...
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