Tài liệu Báo cáo Y học: Puriﬁcation, characterization, cloni

Tài liệu Báo cáo Y học: Puriﬁcation, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d) Fig. 3. Inhibition of ATP and ADP hydrolysis activities of puriﬁed chicken liver ecto-ATPDase by azide. ATP and ADP hydrolysis ... 269) Ó FEBS 2002 Puriﬁcation, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase Aileen F. Knowles 1 , Agnes K. Nagy 2...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... residues of the C. sepium b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, the ... method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, maltose and cyclohexaamylo...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

... applied; the results of a neighbor-joining and a Bayesian approach are presented in Fig. 5. The mono- phyly of the myriapod hemocyanins was recovered by all types of analyses with 100% support. There ... and Bayesian posterior probability (0.87) for an association of the myriapod hemocyanins with the crustacean and insect hemocyanins. All analyses support a pancrustace...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... in supplementary Fig. S2A and P3 in supplementary Fig. S2B). To narrow the range of the possible position of the d-amino acid, chymotrypsin was used to digest natu- ral conomarphin and the synthetic ... analyze the family of 20 structures (Table 3). Figure 3 shows an overlay of the backbone atoms for the 20 structures of conomarphin and l-Phe13-conomarphin (Pro...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... Legrain C, Boyen A & Glansdorff N (1998) Genes and enzymes of the acetyl cycle of arginine biosynthesis in the extreme thermophilic bacterium Thermus thermophilus HB27. Microbiology 144, 479–492. K. ... N-acetyltransferase therefore recycles the acetyl moiety of N-acetyl- ornithine, regenerating N-acetylglutamate in citrulline and arginine biosynthesis. This enzyme is func...

Ngày tải lên : 20/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... ACNATHWSNAAYCAYTGGGA LP1 DAYATTK LP2 WRGDSALGDK LP3 GDNGEDLTGGWY LP4 TEVEGFFK LP5 YPGIYSSSIQDAGQFYSSSGYK R1 RTARAAYTGNCCNGCRTCYTG LP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGCCCAYTT LP7 AWAWALGWDDK LP8 GYHENA LP9 ... middle and C-terminal region of the HdEG66, respectively, from the sequence similarity to the termite cellulase. Then, a forward primer F1 was synthesized on the basis of...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... 9-O-acetyl and 8,9di-O-acetyl forms [41] on de-O-acetylation lost its inhibitory potency completely, thus suggesting the importance of O-acetyl NeuAc in the binding afﬁnity of the lectin. The afﬁnity ... agglutinated only a limited range of erythrocytes. Out of 12 erythrocyte types tested the lectin could agglutinate only six erythrocyte types (Table 2). Our study on the...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and the three ... other combinations of phosphatases and interme- diary phosphorylated enzymes. Therefore, kinetic data on the dephosphorylation of autophosphorylated ATP-cit...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Identiﬁcation of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

... corresponding genes of the A201A biosynthetic pathway, may be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The puromycin biosynthetic gene cluster ... GDP- D -mannose and other hexose dehydratases (data not shown) (Fig. 3). A variety of these enzymes synthesize 4-keto-6-deoxy-GDP- D -mannose, a key intermediate in the...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... the physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulﬁde of coenzyme M and coenzyme B, or by the homodisulﬁdes of these two coenzymes. Also potassium ... Further studies are necessary to elucidate the nature of the extractable heme present in this enzyme. The oxidation of the heme groups of the enzyme by various com...

Ngày tải lên : 21/02/2014, 03:20

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