... the elongation of carbohydrate chains by appropriate glyco-syltransf erase s. Changes in the activities of NSTs mayaffect the structure of oligosaccharide chains by affecting the availability of substrates ... YPH500, and a t ransformant wasobtained. We prepared the microsomes from the transfor-mant, and analyzed them for the presence of the DmNSTprotein by Western blot analysis using anti-HA Ig (Fig. ... Biochemistry, University of Ken-tucky Medical Center, College of Medicine, Lexington, KY, USA.Present address: Department of Applied Chemistry, KogakuinUniversity, Nishi-Shinjuku, Shinjuku-ku,...