Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf
Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf
... activity of thesmaller form of the bifunctional e nzyme was far lowerthan that of the larger form of the enzyme [11]. This isreminiscent of the enzyme from rat liver in which partialproteolysis ... 315–395.Ó FEBS 2002 Spinach 6PF2K/Fru-2,6-P2ase (Eur. J. Biochem. 269) 1277 Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves Jonathan ... 3-phosphoglycerate i n more d etail by e xamining theeffect of this compound on the kinetic response of 6PF2Kactivity to varying substrate concentrations. The activitydisplayed normal hyperbolic kinetics...
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Tài liệu Báo cáo Y học: Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 potx
... purified by TLC chromatography [16] and itsconcentration was measured by the phosphate analysis [17].Glyceraldehyde phosphate, dihydroxyacetone phosphate,sn-glycerol-2-phosphate, and dihydroxyacetone ... activity w asobserved t oward glyceraldehyde phosphate, Gro3P,glycer-ol-2-phosphate (Gro2P), Gr o, and dihydroxyacetone. Thisenzyme efficiently catalyzed the NADH- and NADPH-dependent dihydroxyacetone ... Gro1P dehydrogenase of A. pernixwas purified to homogeneity by a combination of ionexchange, hydrophobic, and gel chromatography. Thepurification procedure yielded approximately 4.4 mg of protein...
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Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot
... Deyashiki, Y. , Hara, A.& Mitsui, Y. (1998) Crystal structure of the ternary complex of mouse lung carbonyl reductase at 1.8 A˚resolution: the structuralorigin of coenzyme specificity in ... Zhang,L.,Ahvazi,B.,Szitner,R.,Vrielink,A.&Meighen,E.(1999) Change of nucleotide specificity and enhancement of cat-alytic efficiency in single point mutants of Vibrio harveyi aldehydedehydrogenase. Biochemistry 38, 11440–11447.33. Busch, ... 2002The effects of low pH on the properties of protochlorophyllideoxidoreductase and the organization of prolamellar bodies of maize (Zea mays)Eva Selstam1, Jenny Schelin1, Tony Brain2and...
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Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf
... freeenergy parameters from the temperature-dependent ther-modynamic data. This improvement will enhance theaccuracy of the predictions of the secondary or tertiarystructures for nucleotide hybrids ... Technology Research Center and2Department of Chemistry, Faculty of Science and Engineering, Konan University,Okamoto, Higashinada–ku, JapanA clear difference in the enthalpy changes derived from spectroscopic ... initiation at physiological tem-perature were extracted from the temperature-dependentthermodynamic data of the 41 RNA/DNA hybrids.Keywords: heat capacity change; temperature-dependentthermodynamics;...
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Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 refined models suggest environment-dependent induction of alternative bent ⁄extended resting states doc
... model of the human platelet integrin aIIbb3based on electroncryomicroscopy and x-ray crystallography. Proc NatlAcad Sci USA 99, 14059–14064.9 Iwasaki K, Mitsuoka K, Fujiyoshi Y, Fujisawa Y, Kikuchi ... implementation of SOMO (SOlution MOdeller) inthe UltraScan analytical ultracentrifugation data analy-sis suite: enhanced capabilities allow the reliable hydro-dynamic modeling of virtually any kind of biomacromolecule. ... a cytoplasmic domain [1–3].The integrin activation mechanism is regulated byconformational changes, the details of which have notyet been fully elucidated [2,3]. X-ray crystallographyKeywordsblood...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... plasticity of the active site gorge of D70G compared with that of the wild-type enzyme foracylation with neutral ester.Effects of benzalkonium on ATMA hydrolysisHydrolysis of ATMA by wild-type ... acti-vator of hydrolysis for neutral substrates and an inhibitor of hydrolysis forpositively charged substrates. The affinity of D70G for tyramine was lowerthan that of the wild-type enzyme. Tyramine ... the hydrolysis of o-NAC is acylation [35], it follows that the activatingeffects of tyramine take place at the level of acylation.For the hydrolysis of o-NPA, both acylation and deac-ylation...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... Contribu-tions of 2¢-hydroxyl groups of the RNA substrate tobinding and catalysis by the Tetrahymena ribozyme. Anenergetic picture of an active site composed of RNA.Biochemistry 32, 8299–8311.23 ... University of Kentucky, Lexington, KY, USAWe previously reported that a group I intron-derivedribozyme from Pneumocystis carinii can catalyze theexcision of a targeted sequence from within ... ligation by an efficient RNAligase ribozyme. Biochemistry 39, 3115–3123.19 Shih I & Been MD (2000) Kinetic scheme for inter-molecular RNA cleavage by a ribozyme derived from Kinetics of the...
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Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt
... of PS, with consequent loss of plasma membrane asymmetry, and depletes newlysynthesized poly PIs. The simultaneous impairment of glycolysis and mitochondrial respiration was accom-panied by ... signal of this protein consists of a polybasic C-terminal sequence of six contiguous lysines and a farnesyl-ated cysteine. Results from biophysical studies in model systems suggestthat hydrophobic ... cleaved by an endopeptidase at thecytoplasmic leaflet of the endoplasmic reticulum (ER),and finally the newly formed free carboxyl group of the C-terminal farnesylcysteine is carboxylmethylated[3]....
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... only known human glycosylated type IIcystatin. In addition to two disulfide bonds, commonto all type II cystatins, cystatin F contains two addi-tional cysteines in positions 1 and 37 (cystatin ... reside intracellularly in lysosomes or lyso-some-like organelles [18].The aim of our study was to identify potential tar-gets of cystatin F among endogenous lysosomal cys-teine proteases. ... twofold molar excess of papain.The residual activity of papain was measured with Z-FR-AMC. Rel-ative activity of cystatin F is shown, from 0% (uninhibited enzyme)to 100% (the lowest activity...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... Honda Y, Kitaoka M, Tokuyasu K, Sasaki C, FukamizoT & Hayashi K (2003) Kinetic studies on the hydrolysis of N-acetylated and N-deacytylated derivatives of 4-methylumbelliferyl chitobioside by ... it cata-lyzed hydrolysis through a retaining mechanism. The enzyme exhibitedendo characteristics, requiring a minimum of two glycosidic bonds forhydrolysis. The kinetics of hydrolysis revealed ... Stereo-chemistry of chitin hydrolysis by a plant chitinase ⁄ lyso-zyme and x-ray structure of a complex with allosamidin:evidence for substrate assisted catalysis. Biochemistry34, 15619–15623.16...
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