Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf

... activity of the smaller form of the bifunctional e nzyme was far lower than that of the larger form of the enzyme [11]. This is reminiscent of the enzyme from rat liver in which partial proteolysis ... 315–395. Ó FEBS 2002 Spinach 6PF2K/Fru-2,6-P 2 ase (Eur. J. Biochem. 269) 1277 Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatas...

Ngày tải lên: 22/02/2014, 04:20

... purified by TLC chromatography [16] and its concentration was measured by the phosphate analysis [17]. Glyceraldehyde phosphate, dihydroxyacetone phosphate, sn-glycerol-2-phosphate, and dihydroxyacetone ... activity w as observed t oward glyceraldehyde phosphate, Gro3P,glycer- ol-2-phosphate (Gro2P), Gr o, and dihydroxyacetone. This enzyme efficiently catalyzed the NADH- and NADPH- dependent di...

Ngày tải lên: 22/02/2014, 04:20

... Deyashiki, Y. , Hara, A. & Mitsui, Y. (1998) Crystal structure of the ternary complex of mouse lung carbonyl reductase at 1.8 A ˚ resolution: the structural origin of coenzyme specificity in ... Zhang,L.,Ahvazi,B.,Szitner,R.,Vrielink,A.&Meighen,E. (1999) Change of nucleotide specificity and enhancement of cat- alytic efficiency in single point mutants of Vibrio harveyi aldeh...

Ngày tải lên: 22/02/2014, 04:20

... free energy parameters from the temperature-dependent ther- modynamic data. This improvement will enhance the accuracy of the predictions of the secondary or tertiary structures for nucleotide hybrids ... Technology Research Center and 2 Department of Chemistry, Faculty of Science and Engineering, Konan University, Okamoto, Higashinada–ku, Japan A clear difference in the enthalpy cha...

Ngày tải lên: 22/02/2014, 07:20

... model of the human platelet integrin a IIb b 3 based on electron cryomicroscopy and x-ray crystallography. Proc Natl Acad Sci USA 99, 14059–14064. 9 Iwasaki K, Mitsuoka K, Fujiyoshi Y, Fujisawa Y, Kikuchi ... implementation of SOMO (SOlution MOdeller) in the UltraScan analytical ultracentrifugation data analy- sis suite: enhanced capabilities allow the reliable hydro- dynamic modeling...

Ngày tải lên: 18/02/2014, 04:20

... plasticity of the active site gorge of D70G compared with that of the wild-type enzyme for acylation with neutral ester. Effects of benzalkonium on ATMA hydrolysis Hydrolysis of ATMA by wild-type ... acti- vator of hydrolysis for neutral substrates and an inhibitor of hydrolysis for positively charged substrates. The affinity of D70G for tyramine was lower than that of the wi...

Ngày tải lên: 18/02/2014, 17:20

... Contribu- tions of 2¢-hydroxyl groups of the RNA substrate to binding and catalysis by the Tetrahymena ribozyme. An energetic picture of an active site composed of RNA. Biochemistry 32, 8299–8311. 23 ... University of Kentucky, Lexington, KY, USA We previously reported that a group I intron-derived ribozyme from Pneumocystis carinii can catalyze the excision of a targeted sequen...

Ngày tải lên: 18/02/2014, 18:20

... of PS, with consequent loss of plasma membrane asymmetry, and depletes newly synthesized poly PIs. The simultaneous impairment of glycolysis and mitochondrial respiration was accom- panied by ... signal of this protein consists of a polybasic C-terminal sequence of six contiguous lysines and a farnesyl- ated cysteine. Results from biophysical studies in model systems suggest tha...

Ngày tải lên: 19/02/2014, 00:20

... only known human glycosylated type II cystatin. In addition to two disulfide bonds, common to all type II cystatins, cystatin F contains two addi- tional cysteines in positions 1 and 37 (cystatin ... reside intracellularly in lysosomes or lyso- some-like organelles [18]. The aim of our study was to identify potential tar- gets of cystatin F among endogenous lysosomal cys- teine proteases....

Ngày tải lên: 19/02/2014, 17:20

... Honda Y, Kitaoka M, Tokuyasu K, Sasaki C, Fukamizo T & Hayashi K (2003) Kinetic studies on the hydrolysis of N-acetylated and N-deacytylated derivatives of 4-methylumbelliferyl chitobioside by ... it cata- lyzed hydrolysis through a retaining mechanism. The enzyme exhibited endo characteristics, requiring a minimum of two glycosidic bonds for hydrolysis. The kinetics of hydroly...

Ngày tải lên: 20/02/2014, 01:20