Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx

... [33], the issue of the partitioning of the glyoxylate cycle in cells grown under fatty acid- medium conditions has hitherto remained open. We showed here that one of the key glyoxylate-cycle enzymes, ... Application of the an tibody to thin se ctions of wild-type cells grown on oleic acid medium Fig. 1. SKL is required to direct Mls1p to the peroxi...

Ngày tải lên: 22/02/2014, 04:20

... target exon, respect- ively. Given the small size of introns 4 and 10 , primers C45U/C45L and C1 011 U/C1 011 L were designed to amplify t ogether e xons 4 and 5 and exons 10 and 11 , respectively. Sequencing ... the same buffer by using 1 mU enzyme/mg casein at 37 °C for 18 h; these c onditions have been previously shown to p roduce complete d ephosphory- lation of the sa...

Ngày tải lên: 22/02/2014, 04:20

... 10 .8 39.6 10 .8 Thr 0.9 1. 1 1. 4 1. 1 Ser 3.9 4.0 3.6 4.4 Glx 2.2 2.2 4.3 2.2 Gly 37.6 28.7 29.6 31. 5 Ala 30.0 31. 8 6.5 30.5 Pro 1. 5 1. 1 1. 2 1. 3 Val 2.2 2 .1 1.4 1. 9 Met 1. 2 1. 1 1. 3 1. 0 Ile 1. 2 1. 3 ... [70–72]. One of the obvious reasons is the solubility of the aspartic acid- rich matrix under the conditions imposed by the decalcification step. A...

Ngày tải lên: 21/02/2014, 01:21

... mutated forms (C 110 )13 1, H 110 )13 1, S 110 )13 1); two 18 amino- acid long peptides (R 114 )13 1, C 114 )13 1) missing amino acids 11 0 11 3 (Leu-Gly-Val-Val); one 13 amino -acid long peptide (big-h3 11 0 )12 2) excluding ... precipitation, without dialysis. None of the TGFBI protein 110 )13 1 peptides analyzed (C 110 )13 1, H 110 )13 1, S 110 )13 1 and R 110 )13 1) displayed a...

Ngày tải lên: 21/02/2014, 01:21

... corresponding to the 12 –700 bp region of the sequence of cDNA # 911 (one XhoI site in the cDNA #4 21 is located in the MCS of BlueScript SK- vector, and another XhoI site is located in the adaptor sequence ... inhibits the ability of CK2 a subunit to phosphorylate calmodulin and this inhibition can be overcome by the polylysine It was shown that in contrast to...

Ngày tải lên: 21/02/2014, 15:20

... seen concomitant with the reduced state at 15 15 cm )1 and the mode typical for the deprotonated form at 14 98 cm )1 , indicating the protonation of a tyrosine residue with the reduction of the protein. ... typically above 17 10 cm )1 , the exact absorption depending on the hydrogen bonding. A negative mode is present at 17 14 cm )1 , which shifts to 17 16...

Ngày tải lên: 21/02/2014, 15:20

... group to this myristoylation site, it is interesting to note that a mutation leading to the loss of a functional myristoylation site could determine the alteration of the local molecular environment ... by mutations disturbing the organi- zation of the enzyme complex, or by mutations in another protein of the enzyme complex. In order to improve our Table 3. Quanti®...

Ngày tải lên: 22/02/2014, 04:20

... & Ball, S.G. (19 95) Storage, photosynthesis, and growth: the conditional nature of mutations affecting starch synthesis and structure in Chlamydomonas. Plant Cell 7, 11 17– 11 27. 29. Ball, S.G., ... their strong hybridization with probe CD142 (GB 911 , GB 111 4 and GB1 411 ). Only GB 911 gave pheno- typic complementation of the sta2 -1 mutant strain (see Results). This prompt...

Ngày tải lên: 22/02/2014, 07:20

... precursor of fatty acids. The initial and committed step of de novo fatty acid synthesis is the carboxylation of acetyl-CoA to form malonyl-CoA, catalyzed by ACC – the key reg- ulatory enzyme in the ... entry of fatty acid into mitochon- dria. Thus, the steady-state level of malonyl-CoA in these tissues is determined by the relative activities of ACC and malonyl-...

Ngày tải lên: 14/02/2014, 22:20

... Human MT 1 2 7 6 5 4 11 8 3 10 9 3a 2a 4a 1a B C Fig. 1. (A) Sequence of the a domain of rhMT-1a, showing the connectivities of the four divalent metal cations to the 11 cystei- nyl sulfurs. The numbering ... initially to the a domain, thereby lead- ing to formation of the M 4 a cluster prior to formation of the M 3 b cluster, demonstrating that the...

Ngày tải lên: 19/02/2014, 00:20