Tài liệu Báo cáo Y học: Interallelic complementation provides genetic evidence for the multimeric organization of the Phycomyces blakesleeanus phytoene dehydrogenase doc

... carotenoid pathway to b-carot ene proceeds via three enzymatic steps carried out by the enzymes phytoene synthase, phytoene dehydrogenase and lycopene cyclase. The enzyme phytoene dehydrogenase is able ... Interallelic complementation provides genetic evidence for the multimeric organization of the Phycomyces blakesleeanus phytoene dehydrogenase Cat...

Ngày tải lên: 22/02/2014, 04:20

... were exclusively heterozygous M/F a t the a s1 -Cas locus. There- fore, analysis o f their transcripts could have been rather difficult because of the occurrence of at least nine different forms of messenger ... phos- phatase hydrolysis), corresponding to that expected for the F variant. This result is the first evidence for the heterozy- gous status (M/F) of the indiv...

Ngày tải lên: 22/02/2014, 04:20

... methyl groups are always introduced, one slowly by Dnmt3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast. Therefore, this effect can only double the rate of DNA methylation, ... novo methylation of DNA [15]. Interestingly, the de novo activity of Dnmt1 is stimulated by binding of methylated DNA to an allosteric site located in the N-terminal domain...

Ngày tải lên: 21/02/2014, 01:21

... induction. Stability of citral lyase activity The activity and stability of citral lyase was dramatically affected by the addition of 20% (v/v) glycerol and 1 m M EDTA. When glycerol and EDTA were added before disrupting ... as the standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in the citral lyase acti...

Ngày tải lên: 21/02/2014, 01:21

... followed the kinetics of amylose synthesis over a 5-day period of nitrogen starvation and measured the amounts of starch, amylose, the k max of the starch fractions, the degree of crystallinity and the ... rate of amylose synthesis accounts for most polysaccharide synthesis. At this stage the rate of amylopectin synthesis has become minimal and it is difficult to...

Ngày tải lên: 22/02/2014, 07:20

... ATP formation and the hydrolysis of ATP by the uncoupler-stimulated ATPase [50]. There- fore, as seen in Fig. 5, ATP levels may drop substan- tially after CCCP treatment. The results of the current ... [12], the assembly of the apoptosome. Upon formation of this complex, caspase-9 acquires the ability to trigger the processing and activation of the downstream cas-...

Ngày tải lên: 16/02/2014, 09:20

... members of the ERF ⁄ AP2 family. (A) Y2 H assays demonstrate the assembly of BPM1(1–189) and BPM3 with RAP2.4 and RAP2.13, respectively. (B) Schematic drawing of the BPM1 fragment used for the Y2 H ... lower parts of the hypocotyl (a). Rosette leaves were stained in the vascular tissue of the leaf blade, whereas the petiole and older parts of the midrib remai...

Ngày tải lên: 18/02/2014, 06:20

... is an essential regulatory enzyme in co-enzyme A biosynthesis, catalyzing the cytosolic phosphorylation of pantothenate (vitamin B5), N-pantothenoylcysteine and pantetheine [20]. PANK2 is also ... the genes involved in the genetics of Lewy body disease, have in common the fact that they impinge on ceramide metabolism. One shortfall of the present theory is the lack of neur...

Ngày tải lên: 18/02/2014, 14:20

... on them even for free. MTurk requesters cannot however rely on this voluntary workforce. From MTurk Forums it is clear that some of the workers rely on the money they get from completing the ... per- form a variety of simple tasks. With tools provided by Amazon.com, the employer creates tasks, and up- loads them to the MTurk Web-site. Workers can then browse the tasks and, i...

Ngày tải lên: 20/02/2014, 09:20

... activity could be detected. Enzyme activity test Phenol hydroxylase activity was estimated polarographically modified from [2] by means of a Clark-type electrode (YSI Model 5300). The phenol hydroxylase ... the isolation and characterization of the regulatory moiety of the multicomponent enzyme phenol hydroxylase from Acinetobacter radioresistens S13 grown on phenol as the only...

Ngày tải lên: 21/02/2014, 00:20