Tài liệu Báo cáo Y học: Expression and characterization of a

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... 5¢-TTGGATGGTATTGATTTTAACATAGAGCATGGTTCAACC-3¢ Anti-sense strand 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢ Glu127Ala Sense strand 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢ Anti-sense strand 5¢-GTACAGGGTTGAACCATGCGCTATGTCAAAATCAATACC-3¢ Asp125Ala/Glu127Ala ... 5¢-GTACAGGGTTGAACCATGCGCTATGTCAAAATCAATACC-3¢ Asp125Ala/Glu127Ala Sense strand 5¢-GATGGTATTGATTTTGCCATAGCGCATGGTTCAACCCTG-3¢ An...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

... MgATPase activity at a divalent ion-ATP concentration of 5 m M at pH 7.4. On the other hand, the CaADPase activity is  80% of the MgADPase activity at a divalent ion-ADP concentration of 5 m M (data not ... e.g. extracellular ATP, ADP, and UTP [1]. They may also play a role in regulating substrate concentration of ecto-protein kinases [2]. A substantial literature on...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... cardiac fibrosis in a paracrine manner under ischaemic conditions. Taken together, these findings may improve understanding of the cellu- lar and molecular basis of the anti-inflammatory and paracrine ... secretion of the inflammatory cytokines IL-1b and TNF -a from hypoxia ⁄ SD-stimulated MSCs, as well as the significant anti-inflammatory effects of MSCs, expression...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... Fukuoka, Japan 2 CREST, Japan Science and Technology Agency, Saitama, Japan 3 Department of Urology, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan Members of the NADPH oxidase ... Similarly, the Noxo1b mRNA was most abundant in the brain among the fetal organs, although it was also present in the thy- mus, liver, and kidney. To compare the amounts...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

... methyl groups are always introduced, one slowly by Dnmt 3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast. Therefore, this effect can only double the rate of DNA methylation, ... with the same amounts of Dnmt1 and Dnmt 3a. All experiments were carried out in triplicate. The ﬁgure shows average values of DNA methylation, the error bars indicate...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx

... tRNA cleavage. Amikacin (Ami) is a semisynthetic aminoglycoside, a derivative of kanamycin A, having the B1 amino group of 2-deoxystreptamine moiety modiﬁed by acylation with 4-amino-2-hydroxybutyric ... pressure was adjusted so that the parent ion intensity was  45% of its initial abundance. DNA strand break analysis The ability of Cu(II)-Ami to induce strand breaks in pl...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc

... carbohydrate chains by appropriate glyco- syltransf erase s. Changes in the activities of NSTs may affect the structure of oligosaccharide chains by affecting the availability of substrates for glycosyltransferases ... 2002 Human and Drosophila UDP-galactose transporters transport UDP- N -acetylgalactosamine in addition to UDP-galactose Hiroaki Segawa*, Masao Kawakita and Nob...

Ngày tải lên : 21/02/2014, 15:20

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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx

... described the isolation and characterization of amphipathic a- helical peptides from the venom of Opistophtalmus carinatus, a scorpion living in southern Africa, and we have made a comparative analysis ... activity [20], parabutoporin and opistoporin 1 were tested on Gram- negative and Gram-positive bacteria and their activity was compared with the activity of m...

Ngày tải lên : 21/02/2014, 15:20

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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf

... agrees with the large body of independent data collected on Calb and truncated Calbs by Kumar’s group. This latter work revealed that EF-hands II and VI of Calb do not bind calcium and that Calb binds a ... products of CR I–II. We thank Walter Chazin (Vanderbilt University, Nashville) for critical evaluation of the manuscript and Barbara Zarzycka (Warsaw) for her technic...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, the active site of the C. sepium b-amylase most probably consists of a cleft located between the barrel ... against b-amylase and C. sepium RNase-related protein (CalsepRRP) Polyclonal antibodies were raised against b-amylase and the unglycosylated isoform of CalsepRRP...

Ngày tải lên : 22/02/2014, 07:20

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