Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T thermophilus docx

... a number of enzymes, which show deviations to the centrally discussed oxidases. The study of these aberrant systems is important to understand the principles of these enzymes. The cytochrome c oxidase ... labeling experiments. Interestingly potential titrations of the enzyme show a slightly different redox-dependent behavior. It may be suggested that the stronger coopera...

Ngày tải lên: 21/02/2014, 15:20

... fluorescence intensity, F o is the fluorescence intensity at the start of the titration, F ¥ is the fluorescence intensity at saturating concentration of ligand, P the total protein concentration, and L ... due to minor alterations in the active site region that affect catalysis but not substrate binding. On the other hand, the statisti- cal comparison between the structu...

Ngày tải lên: 21/02/2014, 01:21

... A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and the ... environment of the N-phosphate of protein-bound phosphohistidine can influence its sensitivity to a phosphatase. Of potential interest in such a context was the...

Ngày tải lên: 21/02/2014, 01:21

... The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustrated schematically at the top. In the upstream region of the CST7 gene, the ATG (arrowhead) at position 0 is the ... interesting in this context. Although a detailed study of the cystatin F transport route was beyond the scope of the present investigation, the fact that cystatin F is...

Ngày tải lên: 21/02/2014, 01:21

... Fig. 1) terminated the first part corres- ponding to the present-day N-terminal domain. Conse- quently, the salt bridge may play an important role in the proper attachment of the N- and C-terminal ... 3 and 4. Both methods support a constitutive expression of AtP1, AtP2, AtP9, AtP17, AtP4 and AtPCb. It is noteworthy that these AtPs represent highly diverse structures (Fig....

Ngày tải lên: 21/02/2014, 01:21

... activity of (Val1,Thr3,Thr6)- bradykinin in both of the smooth muscle preparations employed in this study, in contrast to the activity observed for the Ala3,Thr6-substituted variant (Figs 7 and ... substituted with a prolyl residue. The propeptide convertases in this species, appear to disregard the -Arg-Lys- doublet two positions proximal to this substitution and rather cle...

Ngày tải lên: 21/02/2014, 15:20

... differences in these cells in the distribution of TRAF4 between the cytoplasmic and the nuclear fraction (data not shown). This discrepancy cannot be attributed to the GFP part of the various TRAF4 fusion proteins, ... Analysis of the various TRAF4 deletion mutants showed that the C-TRAF domain part of the TRAF domain is sufficient to direct the molecule into the...

Ngày tải lên: 21/02/2014, 15:20

... points toward slight differences in the conformation of the chelate rings. The characteris tic alternate pattern of the d–d bands in the C D s pectra is dictated by the c onformation o f the ... conformation of the chelate system in the protein is also intermediate between these two models. The Cu(II) stabilities at site II were measured directly, by taking advantage...

Ngày tải lên: 22/02/2014, 04:20

... novo synthesis of PtdIns is most active. Thorough enzymatic characterization of synthesis and exchange has attemptedtounderstandifbothreactionsaremediatedby the same enzyme, but only one study has ... clear, for the first time, that a PtdIns synthase from Arabidopsis is able to catalyse both de novo synthesis of PtdIns and the exchange of the inositol moiety, but it is st...

Ngày tải lên: 22/02/2014, 04:20

... available to make this determination. In an attempt to test the simple hypothesis that class II XI stability at high temperatures correlates with its N þ Q content, independent of the growth temperature ... activity and stability. Our results show that for class II XIs, N þ Q content relates to the enzyme’s functional temperature range and that BLXI thermostability is also directl...

Ngày tải lên: 22/02/2014, 07:20