Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain Yoko Kobayashi, Toshiaki Nakajima and Ituro Inoue Division of Genetic Diagnosis, Institute of Medical ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other f...

Ngày tải lên: 21/02/2014, 15:20

... concentration of 3.4 lm. Thermophilicity and thermostability Thermophilicity was evaluated in the temperature range 40–90 °C by measuring the polymerase activity of the Rep245 under standard conditions ... Interestingly, this property is shared by the X family of human DNA polymerases, which includes the TdT enzymes and two additional members, Pol k [29] and Pol l [30]...

Ngày tải lên: 18/02/2014, 18:20

... by Rho kinase and the other by the Ca 2+ -dependent activation of MLC kinase. These two pathways could be activated independ- ently of each other, depending on the type and concen- tration of ... inhibition of MLC phosphorylation, as demon- strated by the experiments with BAPTA, which only diminished MLC phosphorylation by about 50%. The enhanced capability of cAMP of...

Ngày tải lên: 21/02/2014, 01:21

... prepared and analysed as described in A. The molecular weight and position of the two forms of antizyme is indicated on the right. *NS indicates the position of nonspecific proteins recognized by the ... antizyme was rapidly degraded in both the presence and the absence of coexpressed ODC (Figs 2C and D). Similarly, we have noted that antizyme that was induced b...

Ngày tải lên: 22/02/2014, 04:20

... of the loop indicated by the letter a. Fig. 5. Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and ... indicating that the environ- ment of tryptophan residues is essentially conserved in the mutant enzyme. On the other hand, the absence of major differences i...

Ngày tải lên: 21/02/2014, 01:21

... some of the key findings that have led to our current under- standing of the molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of ... (blue) structures shows the dif- ferent conformations of the main chain of the a2–a3 loop, see arrows [49]. For the sake of clarity, only the FAD and th...

Ngày tải lên: 14/02/2014, 14:20

... conserved motif of Gly–Gly–X–Gly. The second glycine of this motif (Gly374) is known to make a hydrogen bond with the pyrophosphate moiety of the FAD of the next monomer in the tetramer [16]. However, the ... 3-methylcrotonyl-CoA. IVD is a member of the acyl-CoA dehydrogenase (ACAD) family of enzymes, all of which share significant sequences and employ a similar e...

Ngày tải lên: 18/02/2014, 04:20

... ladders of helices running through the domain [18,19]. The crystal structure of the human Pumilio ⁄ NRE complex demonstrated that each repeat of the PUF domain recognizes a single nucleotide in the ... cells. Screening of the Arabidopsis RNA library Yeast strain YBZ-1 [40] was co-transformed with APUM-2 protein hybrid and the hybrid RNA plasmids. Cells were gro...

Ngày tải lên: 18/02/2014, 06:20

... stages of development. The amounts of pro-b-conglycinin and proglycinin are considered to be nearly equivalent to the synthesis levels of both b-conglycinin and glycinin, as pro-b-conglycinin and ... folding and quality control of nascent polypeptides [25]. In soybean cotyledon, large amounts of seed stor- age proteins such as glycinin and b-conglycinin are synthesized...

Ngày tải lên: 18/02/2014, 11:20

... 481–485. 10 Ollivaux C & Soyez D (2000) Dynamics of biosynthe- sis and release of crustacean hyperglycemic hormone isoforms in the X-organ-sinus gland complex of the crayfish Orconectes limosus. ... Toullec JY & Soyez D (2002) Enkephalinergic control of the secretory activity of neu- rons producing stereoisomers of crustacean hyperglyce- mic hormone in the eyestal...

Ngày tải lên: 18/02/2014, 11:20