Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot

... 2002 Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases Brenda J. Blacklock and Jan G. Jaworski Department of Chemistry and Biochemistry, Miami ... conver- sion of a hydroxylase to a desaturase [26]. The objective of our work was to examine the substrate specificity of A. thaliana and B. napus FAE1 KCSs and...

Ngày tải lên: 21/02/2014, 15:20

... Series 4) and found only marginal differences for any of the enzyme substrate pairs [Fig. 5C shows an example of DNA glycosylase activity of OGG1 with the range of poly(ethylene glycol) 8000 and 0 ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl 2 . (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of...

Ngày tải lên: 18/02/2014, 18:20

... 4¢-phosphopantothenoyl-CMP by cysteine is inhibited. PPC synthetase binds derivatives of cysteine To study the substrate specificity of His-CoaB, L -serine, L -alanine, cysteamine, L -cysteine methyl ester, ... covalently linked to an adenylyl group, whereas it is covalently linked to a serine hydroxyl group in acyl carrier proteins. 4¢-Phosphopantetheine is also cofactor of en...

Ngày tải lên: 19/02/2014, 12:20

... that the lower activity of the mutant may be ascribed to the loss of an acceptor hydrogen bond to a metal-bound hydroxide, as a consequence of replacement of a carboxylate oxygen with an amide group. MATERIALS ... mutation of Asp153 by asparagine, the catalytic activity of agmatinase (agmatine ureohydrolase, EC 3.5.3.11) from Escherichia coli was reduced to about 5% of wil...

Ngày tải lên: 21/02/2014, 01:21

... 2C-methyl- D-erythritol 4-phosphate can be converted into a cyclic diphosphate by the consecutive action of 4-diphosphocytidyl-2C-methyl- D- erythritol synthase, 4-diphosphocytidyl-2C-methyl- D-erythritol kinase ... to the 2C-methyl- D-erythritol 2,4-cyclodiphosphate Fig. 3. 13 C NMR signals of 2C-methyl-D-erythritol 2,4-cyclodiphosphate obtained by incubation of [U- 13 C 5 ]2C-meth...

Ngày tải lên: 22/02/2014, 07:20

... terminal enzyme of the respiratory chain in mitochondria and many prokaryotes. As an integral membrane protein it catalyzes the reduction of dioxygen to water using electrons from cytochrome c. Four ... heme c. This potential differs for about 50 mV from the midpoint potential of isolated cytochrome c, indicating the possible shifts of the cytochrome c potential when bound to cytoc...

Ngày tải lên: 21/02/2014, 15:20

... [18]. Enzymatic hydrolyses Trypsin (Boehringer Mannheim) hydrolysis was carried out in 0.4% NH 4 HCO 3 ,pH8.5,at37°C, for 4 h, in a substrate/ enzyme ratio of 5 0 : 1 (w/w). Dephosphorylation with ... freeze- drying. Liquid chromatography/mass spectrometry analysis of proteins and peptides The whole caprine casein samples were fractionated by the procedure of J aubert and M artin [21],...

Ngày tải lên: 22/02/2014, 04:20

... deoxycytidine kinase; dGK, deoxyguanosine kinase; dN, deoxyribonucleosides; dT, deoxythymidine; dU, deoxyuridine; dC, deoxycytidine; dA, deoxyadenosine; dG, deoxyguanosine; hTK1, human thymidine ... sub- strate specificity and phosphorylates only thymidine (dT) and deoxyuridine (dU), whereas TK2 also phos- phorylates deoxycytidine (dC). dCK phosphorylates dC, deoxyadenosine (dA) and deoxygua...

Ngày tải lên: 30/03/2014, 20:20

... agmatine not only is practically not hydrolysed by wild-type arginase II, but it is also very poorly inhibitory to this enzyme form. Thus, the altered specificity most prob- ably reflect an active ... activity of Asn149Asp on agmatine was only about 5% of the arginase activity of the wild- type enzyme. As measured by k cat ⁄ K m , the catalytic efficiency of the Asn149Asp variant was...

Ngày tải lên: 20/02/2014, 02:21

... The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group into an aldehyde group in the presence of a secondary hydroxy group. J. Prakt. Chem. ... Methyl 2,3-anhydro-a- D -allopyr- anoside (2a) and methyl 3,4-anhydro-a- D -galactopyrano- side (2b) were obtained by desilylation of 1 mmol of the 6-O-[dimethyl-(1,1,2-trimethylpro...

Ngày tải lên: 21/02/2014, 01:21