Tài liệu Báo cáo Y học: CK2btes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster potx

... 26 9) 1 427 CK2btes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster Alla I. Kalmykova 1 , Yuri Y. Shevelyov 1 , Oksana O. Polesskaya 1, *, ... subunit a re the canonical features of the regulatory s ubunit of CK2. CK2btes protein interacts with CK2 a subunit in yeast two-hyb...

Ngày tải lên: 21/02/2014, 15:20

... above 72 8C (Fig. 2) . As both the E a of inactivation and the temperature at which the enzyme starts inactivating at a measurable rate increase from the apoenzyme to the Mn 2 -containing enzyme, ... corresponding dialysis buffer. The dialysis buffer was used to generate the baseline. The enzyme containing both Mg 2 and Co 2 was dialyzed against buffer A, then s...

Ngày tải lên: 22/02/2014, 07:20

... commer- cially available bacitracin preparations [31]. Here, we have tested the effect of bacitracin in a variety of in vitro assays for PDI activity. On the basis of the BPTI refolding assay and peptide ... activity were examined using the insulin precipitation assay. In the noncatalyzed assay, the disulfides of insulin are reduced by dithiothreitol, causing the...

Ngày tải lên: 16/02/2014, 14:20

... b-galactosidase. b-1,4-Endogalactanases cleave within the galactan moiety of type I arabinogalactan, releasing D -galacto-oligosaccharides. Bacterial b-1,4-endo- galactanases release mainly galactotriose ... b-(1,4)-endogalactanase prepar- ation. 1, 2: purified GALA preparation; 3: molecular mass marker proteins. Fig. 5. HPAEC analysis of the hydrolysis of arabinogalactans by GALA....

Ngày tải lên: 21/02/2014, 01:21

... phosphorylation may be lying upstream of ERK1 /2. Using a serine/threonine kinase SPA assay kit, the capability of NAMI -A to affect the ERK1 /2 phosphoryla- tion activity was also assessed. Consistent ... that NAMI- A- mediated inhibition of ERK1 /2 phosphorylation may be lying upstream of ERK1 /2. Recent observations indicate that activation of Raf by PMA may trigger...

Ngày tải lên: 21/02/2014, 01:21

... and the dynamics of their synthesis. In any case, the coordinated r egulation of 4 CL3 or 4 with a ll other known enzymes of phytoalexin biosyn- thesis in soybean [24 ] indic ate that at least these ... 20 02 substituted cinnamates including sinapate. This catalytic property is shared by only the minority of 4CL isoforms studied to date from any plant. As the cDNA enco...

Ngày tải lên: 22/02/2014, 04:20

... Chemical Research), Japan; 2 Department of Animal Resource Science/Veterinary Medical Sciences, The University of Tokyo, Japan The Sp family is a family of transcription factors that bind to cis-elements in the ... enzymes (D1 to D5 in Fig. 1A) . Each DNA fragment was inserted into the Nhe1andXho1sites of pGL3-basic (Promega Co., Madison, WI, USA). The integrating o...

Ngày tải lên: 22/02/2014, 07:20

... questioned the paraphyly of the order rodentia using the same data as Graur. In contrast, the distance matrix algorithms again placed the guinea pig together with artiodactyls and primates, thus ... electron transfer protein adrenodoxin indicate dif- ferential sensitivity of CYP11B1 and CYP11B2 as well as the three hydroxylation steps catalysed by CYP11B2 to the availabili...

Ngày tải lên: 22/02/2014, 07:20

... mitochondrial fatty acid-transferring enzyme; MCAD, a representative fatty acid b-oxidation enzyme; PPARa, a key transcriptional regulator of fatty acid genes; UCP3; the cardiac-enriched GLUT4; and ... (Fig. 2A) . In parallel, expression of the genes encoding muscle-type carnitine palmitoyltransfer- ase 1 (mCPT1) and medium-chain acyl-CoA dehydro- genase (MCAD) (both PPARa targe...

Ngày tải lên: 18/02/2014, 16:20

... ctgac ttgc ag AT Intron 2 G7.0 489 CAG gt aaag ctaac ttgc ag GT Al. pompejana Hb Intron 1 B 12. 2 135 GGA gt aagt ctaac accc ag GT Intra Intron 2 G7.0 24 1 GCT gt aagt ctaac tttc ag GA Ar. marina ... 495 GAC gt aagt ataac ctgc ag GA R. pachyptila A2 Intron 1 B 12. 2 495 CCA gt gagt ctaat ttgc ag TG Intron 2 G7.0 639 GAC gt aagc cttat cctc ag AC B2 Intron 1 B 12. 2 531 GAC gt aagc ctta...

Ngày tải lên: 19/02/2014, 02:20