Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt

... Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells Danilo Guerini 1 , Elisabetta Prati 1 , Urvi Desai 2 , Hans Peter ... play a role in mitochondrial uncoupling were o btained using yeast expression sys tems [1,2,11,12]. In contrast to observations of UCP1 overexpression in yeast, t...

Ngày tải lên: 21/02/2014, 15:20

... on the catalytic properties of yeast poly(A) polymerase and on the intracellular role of dinucleoside polyphosphates, a family of compounds of increasing metabolic and regulatory interest [7–11]. MATERIALS ... the synthesis of poly(A) catalyzed by yeast poly(A) polymerase. The relative activity of diadenosine polyphosphates as effectors of the poly(A) synthesis was assayed as i...

Ngày tải lên: 21/02/2014, 01:21

... inhibitory complex can be the result of binding of TFPI to the enzyme–product Xa–VIIa–TF complex and this is the main pathway of inhibition of VIIa–TF during ongoing factor X activation. Analysis of ... the authors of the study [8] introduced a hypothesis of inhibitory action of TFPI on the enzyme–product complex as the predominant pathway of TFPI action (Scheme 2). The seco...

Ngày tải lên: 22/02/2014, 04:20

... residues of the C. sepium b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, ... the activity of the enzyme was measured by the dinitrosalicylic acid method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme in...

Ngày tải lên: 22/02/2014, 07:20

... mycolic acid–arabinogalactan and lipoarabinomannan, as well as a variety of complex glycoli- pids [1]. Among the interesting and important glycolipds are a number of trehalose-containing lipids, such ... methylation analysis to determine linkages, 1 H-NMR to obtain anomeric configu- rations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and a...

Ngày tải lên: 22/02/2014, 07:20

... Germany 2 Department of Orthopedics, University Regensburg, Germany 3 Max-Planck-Institute of Biochemistry, Martinsried, Germany The family of activating enhancer-binding protein (AP)-2 transcription ... Identification of amino acid residues of transcription factor AP-2 involved in DNA binding. J Mol Biol 301, 807–816. 10 Wankhade S, Yu Y, Weinberg J, Tainsky MA & Kan- nan P (...

Ngày tải lên: 18/02/2014, 08:20

... properties. In this work we took advantage of the specific labeling with PLP of Lys288 in E. coli PEP carboxykinase to gain insight into conformational alterations of the protein upon ligand binding in ... characteristics of the native protein, allowing the determination of several pro- tein–ligand dissociation constants, as well as the role of Mg 2+ and Mn 2+ in substrate...

Ngày tải lên: 21/02/2014, 01:21

... of methyl a- D -glucopyranoside (1a)andmethyla- D -manno- pyranoside (1c), methyl 6-deoxy-6-isothiocyanato-a- D - glucopyranoside (1e), b- D -glucopyranosyl isothiocyanate (3c)andb- D -glucopyranosyl ... What cannot be excluded is that dephosphorylation and/or catalytic turnover of EII Glc , rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant...

Ngày tải lên: 21/02/2014, 01:21

... The methylation pattern is created by de novo methylation and demethyla- tion of the DNA, and maintained during mitosis by maintenance methylation [reviews 8,9]. De novo methyla- tion of DNA is ... lead to hypermethylation of promotor regions of tumor suppressor genes in cancer cells and is an important mechanism for cancer progression [11–13]. The mechanism of de novo methyla...

Ngày tải lên: 21/02/2014, 01:21

... polysaccharides, in particular pectin. Two types of arabinogalactan side chains are present in pectin. Type I consists of a chain of b-1,4 linked D -galactopyranose linkages, while type II contains a backbone ... duplicate. The hydrolysis activity of GALA with different arabino- galactans was measured in duplicate by HPAEC and MALDI-TOF MS after incubation of 1 mgÆmL )1 of...

Ngày tải lên: 21/02/2014, 01:21