Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx

... CumOOH, and NADPHwere also obtained from Sigma. Sephadex G-25 was pur-chased from Pharmacia (Uppsala, Sweden). All the othermaterials were of analytical grade and obtained fromBeijing Chemical ... such asinstability, antigenicity and poor availability, muchattention has been paid to its artificial imitation [9,10].In synthetic approaches, an initial attempt is made to synthesize organoselenium ... indicated an increase in mito-chondrial swelling and a decrease in mitochondria integrity.TBARS content in ferrous sulfate ⁄ ascorbate-treatedmitochondria was analyzed by thiobarbituric acid assay[34]....

... 2A, lane 4) and ssDNA-dependentATPase activity (data not shown) sedimented togetherbetween alcohol dehydrogenase and BSA (fraction 11) and gave a molecular mass of 120 kDa with a sedimen-tation ... HDH,human DNA helicase; eIF-4 A, eukaryotic translation initiation factor 4A. Property PDH45 a PDH65bPDH120Molecular mass: SDS/PAGE 45.5 kDa 65 kDa 54 and 66 kDaNative 45.5 kDa 65 kDa 120 kDaOligomeric ... doi:10.1046/j.1432-1033.2003.03532.xss- and double-stranded (ds) DNA and total RNA frompea leaves were prepared by standard methods. NTPs/dNTPs, ATPcS, poly (A) , poly(U), poly(C), poly(G) and yeast tRNA were from Boehringer-Mannheim.[c-32P]ATP...

... by SDS/PAGE and autoradio-graphy. Figure 5 shows that after incubation with labelledMSSAE, membranes contained radioactive protein bothmonomeric and dimeric (lane 1). This indicates that underthe ... containing 1 mgÆmL)1BSA and 25 mMHepes atpH 7.5). At increasing time intervals, cells were washedrepeatedly with NaCl/Piandthentreatedwith0.6MNaClin NaCl/Pifor 5 min at 4 °C to detach ... D’Alessio11Dipartimento di Chimica Biologica and 2Dipartimento di Biologia e Patologia Cellulare e Molecolare ‘L. Califano’,Universita`di Napoli, ItalyThe dimeric structure of seminal ribonuclease (BS-RNase)is...

... may also be related to low formation oftopoII-DNA complexes, which is generally associated with G2arrest and the absence of programmed apoptoticdeath [5]. Be that as it may, daunorubicin and ... Jurkat cells treated with a range ofconcentrations of daunorubicin or WP631 is illustrated inFig. 2. Data were obtained after 24 h (panels A and B) and 72 h (panels C and D). No significant ... D. Anderson Cancer Center, Houston, TX, USAJurkat T lymphocytes were treated with daunorubicin and WP631, a daunorubicin-based DNA binding agent, inexperiments aimed to analyze cellular uptake...

... glycoxidation and lipoxidation reactions with auto-xidation products of ascorbic acid and polyunsaturated fattyacids. FEBS Lett. 437, 24–28.8. Motomiya, Y., Oyama, N., Iwamoto, H., Uchimura, T. & ... indicate that mitochondrial matrix proteinsundergo oxidative and glycoxidative modifications. Thesedamaged proteins accumulate with aging, in parallel with a large decrease in Lon protease activity.Materials ... activity.Materials and methodsAnimalsExperiments were performed on male Wistar rats (WAG/Rij) born and raised in the animal care facilities of theCommissariat a `l’Energie Atomique (CEA Gif-sur-Yvette,France)....

... Inagaki S, Suzuki T, Ohto MA, Urawa H, Horiuchi T,Nakamura K & Morikami A (2006) ArabidopsisTEBICHI, with helicase and DNA polymerase domains,is required for regulated cell division and ... 2-week-oldpAtHELPS:GUS and 35S::GUS transgenic seedlings are shown. The average GUS activity was obtained from at least five independent trans-formants, and each assay was repeated three times. Standard errors are ... growth and survival. To ensure an adequate supply of K+, plants have a num-ber of redundant mechanisms for K+acquisition and translocation [5–7]. In the past decade, several high-affinity...

... pancreaticcancer by alpha-enolase, a novel pancreatic ductaladenocarcinoma-associated antigen. Int J Cancer 125,639–648.30 He P, Naka T, Serada S, Fujimoto M, Tanaka T,Hashimoto S, Shima ... incervix and colon cancer, leukemia, normal pancreaticducts and tumoral pancreatic cells. Fourteen acetylatedlysine residues are common to leukemia, pancreaticcancer and normal pancreas, and one ... (IMMONC), Ricerca SanitariaFinalizzata, Ricerca Sanitaria Applicata; RibovaxBiotechnologies (Geneva, Switzerland) and FondazioneItaliana Ricerca sul Cancro (FIRC).References1 Pancholi V (2001)...

... Whereas manytropical and subtropical species have only limitedcapacities to cope with low temperature, plants fromtemperate climates, such as Arabidopsis thaliana, growat low temperature and can ... high-performanceanion exchange chromatography (HPAEC) with a Carb-oPac PA-1 column on a Dionex (Sunnyvale, CA, USA)T. Na¨gele et al. Systems biology of cold acclimation in A. thalianaFEBS Journal ... during acclimation to low temperature in Arabidopsis thalianaThomas Na¨gele, Benjamin A. Kandel*, Sabine Frana*, Meike Meißner and Arnd G. HeyerBiologisches Institut, Abteilung Pflanzenbiotechnologie,...

... commercially available antibodies, and their glycan alterationwas examined by a lectin microarray. Finally, they were analyzed by multi-stage tandem MS.AbbreviationsAAL, Aleuria aurantia lectin; ... thedynamic mammalian glycome. Proc Natl Acad Sci USA104, 11534–11539.14 Tateno H, Uchiyama N, Kuno A, Togayachi A, SatoT, Narimatsu H & Hirabayashi J (2007) A novel strat-egy for mammalian ... lectin-mediated affinity capture and glycosyl-ation site-specific stable isotope tagging. Nat Protocols1, 3019–3027.21 Natsume T, Yamauchi Y, Nakayama H, Shinkawa T,Yanagida M, Takahashi N &...