Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx

... 2002 A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors Siew Lay Poh 1,2 , Gilles Mourier 1 , Robert Thai 1 , Arunmozhiarasi Armugam 2 , ... first pair was X289 (5¢ TgTgCTACTTgCC CTggAA 3¢)andX191. The second pair was X133 (5¢ TCC AgAAAAgATCgCAA gATg 3¢) [35] and X300 (5¢ AgAgC CAAgCTTTTACT ATCggTT 3¢...

Ngày tải lên: 21/02/2014, 03:20

... CumOOH, and NADPH were also obtained from Sigma. Sephadex G-25 was pur- chased from Pharmacia (Uppsala, Sweden). All the other materials were of analytical grade and obtained from Beijing Chemical ... such as instability, antigenicity and poor availability, much attention has been paid to its artificial imitation [9,10]. In synthetic approaches, an initial attempt is made to synthe...

Ngày tải lên: 19/02/2014, 02:20

... 2A, lane 4) and ssDNA-dependent ATPase activity (data not shown) sedimented together between alcohol dehydrogenase and BSA (fraction 11) and gave a molecular mass of 120 kDa with a sedimen- tation ... HDH, human DNA helicase; eIF-4 A, eukaryotic translation initiation factor 4A. Property PDH45 a PDH65 b PDH120 Molecular mass: SDS/PAGE 45.5 kDa 65 kDa 54 and 66 kDa Native 45....

Ngày tải lên: 20/02/2014, 11:20

... by SDS/PAGE and autoradio- graphy. Figure 5 shows that after incubation with labelled MSSAE, membranes contained radioactive protein both monomeric and dimeric (lane 1). This indicates that under the ... containing 1 mgÆmL )1 BSA and 25 m M Hepes at pH 7.5). At increasing time intervals, cells were washed repeatedly with NaCl/P i andthentreatedwith0.6 M NaCl in NaCl/P i for 5 min at...

Ngày tải lên: 20/02/2014, 11:20

... may also be related to low formation of topoII-DNA complexes, which is generally associated with G 2 arrest and the absence of programmed apoptotic death [5]. Be that as it may, daunorubicin and ... Jurkat cells treated with a range of concentrations of daunorubicin or WP631 is illustrated in Fig. 2. Data were obtained after 24 h (panels A and B) and 72 h (panels C and D...

Ngày tải lên: 20/02/2014, 23:20

... glycoxidation and lipoxidation reactions with auto- xidation products of ascorbic acid and polyunsaturated fatty acids. FEBS Lett. 437, 24–28. 8. Motomiya, Y., Oyama, N., Iwamoto, H., Uchimura, T. & ... indicate that mitochondrial matrix proteins undergo oxidative and glycoxidative modifications. These damaged proteins accumulate with aging, in parallel with a large decrease...

Ngày tải lên: 20/02/2014, 11:20

... Inagaki S, Suzuki T, Ohto MA, Urawa H, Horiuchi T, Nakamura K & Morikami A (2006) Arabidopsis TEBICHI, with helicase and DNA polymerase domains, is required for regulated cell division and ... 2-week-old pAtHELPS:GUS and 35S::GUS transgenic seedlings are shown. The average GUS activity was obtained from at least five independent trans- formants, and each assay was repeated thre...

Ngày tải lên: 14/02/2014, 18:20

... pancreatic cancer by alpha-enolase, a novel pancreatic ductal adenocarcinoma-associated antigen. Int J Cancer 125, 639–648. 30 He P, Naka T, Serada S, Fujimoto M, Tanaka T, Hashimoto S, Shima ... in cervix and colon cancer, leukemia, normal pancreatic ducts and tumoral pancreatic cells. Fourteen acetylated lysine residues are common to leukemia, pancreatic cancer and normal pancreas...

Ngày tải lên: 14/02/2014, 19:20

... Whereas many tropical and subtropical species have only limited capacities to cope with low temperature, plants from temperate climates, such as Arabidopsis thaliana, grow at low temperature and can ... high-performance anion exchange chromatography (HPAEC) with a Carb- oPac PA-1 column on a Dionex (Sunnyvale, CA, USA) T. Na ¨ gele et al. Systems biology of cold acclimation...

Ngày tải lên: 14/02/2014, 22:20

... commercially available antibodies, and their glycan alteration was examined by a lectin microarray. Finally, they were analyzed by multi- stage tandem MS. Abbreviations AAL, Aleuria aurantia lectin; ... the dynamic mammalian glycome. Proc Natl Acad Sci USA 104, 11534–11539. 14 Tateno H, Uchiyama N, Kuno A, Togayachi A, Sato T, Narimatsu H & Hirabayashi J (2007) A novel strat- egy...

Ngày tải lên: 16/02/2014, 08:20