Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf

... bold. b The type of mutation: natural polymorphism (P), mutation appearing only in drug resistance (R, see Fig. 1). If two mutations occurred and one of them was occurring only in drug resistance, ... Stock solutions and dilutions were made in distilled water and the peptide concentrations were determined by amino-acid analysis. Construction and purification of HIV-1...

Ngày tải lên: 21/02/2014, 03:20

... absence of ligand, the WPD loop of PTP1B and RPTPa adopts an open (inactive) conformation, and upon ligand bind- ing, it adopts a closed (active) conformation (Fig. 3A). In oxidized PTP1B [12,13] and ... of InsP 6 to PhyAsr (Table 1). Given the absence of structural changes as a function of ionic strength and ligand binding, the effect of ionic strength on catalytic ac...

Ngày tải lên: 18/02/2014, 18:20

... impact of silencing TRBP and Dicer on the function of the fully paired and mismatched sequences, we wished to examine Fig. 1. Effect of guide strand 5¢ end mismatch on silencing efficacy of siRNAs. ... the Dicer and TRBP complexes are assumed to contain only one molecule each of protein and siRNA. As further confirmation of the identities of the complexes, we showed...

Ngày tải lên: 18/02/2014, 06:20

... Ig-b locus. Acetylation of H3 and H4 histones in cells deleted in region II Acetylation of H3 and H4 histones is enhanced in and around potentially active and transcribed genes A BC Fig. 2. Construction of DT40 ... role of this region in the transcription of the Ig-b gene. Deletion of region III decreased the expres- sion of Ig-b mRNA to 40% of the expression in prede-...

Ngày tải lên: 19/02/2014, 16:20

... several cytoplasmic ligands. It is activa- ted by millimolar concentrations of ATP and micromo- lar Ca 2+ , and inhibited by higher concentrations (> 100 lm)ofCa 2+ and by millimolar Mg 2+ . ... pore [10]. Results about the effect of the luminal (trans) calcium on the activity of the RyR1 are controversial depending on experimental conditions. Inhibition of the channel...

Ngày tải lên: 19/02/2014, 16:20

... term correspondences are estimated, in terms of both computational complexity and the performance of precise estimation of bilingual term correspondences. 1 Introduction Translation knowledge acquisition ... situ- ation. One limitation of the corpus-based trans- lation knowledge acquisition approach is that the techniques of translation knowledge acquisition heavily rely on avai...

Ngày tải lên: 22/02/2014, 02:20

... the formation of hydrogen bond network increase the number of possible configurations and conformation states of the complex. Calculation of energy differences between the states containing different ... different conformations of the isostere of inhibitor fi tting well the electron d ensity were modeled (Fig. 7). In the first conformation, the NH group of Phe i n position P 1 ¢...

Ngày tải lên: 19/02/2014, 16:20

... action of EPA and DHA (Eur. J. Biochem. 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic ... binding elements may contribute to the modu- lation of egr-1 gene expression (Fig. 4B). Effects of n-3 and n-6 PUFAs on the synthesis of caveolin-1 and caveo...

Ngày tải lên: 19/02/2014, 16:20

... Abstract We demonstrate applications of psycholin- guistic and sublexical information for learn- ing Chinese characters. The knowledge about the grapheme-phoneme conversion (GPC) rules of languages ... exploration of using the combination of audio and visual stimuli. In addition to pictograms, more than 80% of Chinese characters are phono- semantic characters (PSCs, hencefo...

Ngày tải lên: 19/02/2014, 19:20

... lid, which has consequences for 26S proteasome peptidase activity. In addition, we demonstrate the essential role of the PCI domain of CSN2 for complex formation and consequences of permanently ... The consequence of permanent Flag-CSN2 overexpression was de-novo assem- bly of the CSN complex connected with an accelerated degradation of p53 and stabilization of c-Jun in B8 c...

Ngày tải lên: 20/02/2014, 01:20