Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
... knowledge, the work described here and in the related publications [1,7] is the first kinetic analysis of the in vitro interactions Cyt f plastocyanin and plastocyanin Fig. 4. Comparison of ionic strength ... Schlarb-Ridley et al.(Eur. J. Biochem. 269) Ó FEBS 2002 Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum Beatrix G. Schlarb-Ridley1, ... transition state revealedpositive activation entropies, suggesting partial desolvation of the interface in the transition state. In comparison withplants, plastocyanin and photosystem I of Phormidium laminosum...
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Tài liệu Báo cáo Y học: Transactivation domains are not functionally conserved between vertebrate and invertebrate serum response factors potx
... promotes interaction between SRF and C/EBPa to activate transcription. In summary, the data obtained in this study indicate the conservation of the SRF DNA-binding and dimerizationdomains during ... anti-(c-myc) Ig. The results obtained indicateco-immunoprecipitation and, therefore, interaction between Fig. 1. Effect of SRF co-expression on the activity of CArG-containing promoters. (A) Activity ... productive interaction with activatingcofactors. Alternatively, inhibition could be explained bydiluting of these cofactors by an excess of nonDNA-boundSRF. This would be the more likely mechanism...
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Tài liệu Báo cáo Y học: Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii ppt
... inhibits PSII activity by binding to the relativelyhydrophobic QBpocket, which is the exit of the electronflow in the PSII complex. According to the increase in the sensitivity to diuron in ... findingwhich may be a key to realizing the role of acidic lipids in photosynthesis. The contribution of SQDG to the maintenance of maxi-mum PSII activity in C. reinhardtii was not observed withSQDG-null ... hf-2withSQDGraisedtheloweredPSII activity. Chemical modifications of SQDG impaired the recovery of PSII activity. The results suggest that SQDG isindispensable for PSII activity in Chlamydomonas by main-taining...
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Tài liệu Báo cáo khoa học: RMI1 deficiency in mice protects from diet and genetic-induced obesity pptx
... aphenotype indicative of resistance to diet-induced and genetic obesity. The mutation of the 0283 strain is in the RMI1 gene.RecQ-mediated genome instability 1 (RMI1) hasrecently been identified as ... that the level of RMI1 expression in the fat and liver isassociated with development of obesity.RMI1 is the component of the BLM helicase com-plex that maintains complex stability and aids in the maintenance ... hyperinsulinemic and hyperglyce-mic. Introduction of the lethal yellow agouti gene (A y )into KK mice resulted in a congenitally lethal yellowobese KK mouse strain (KK-A y ), which exhibits...
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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... viaproteasome-mediated proteolysis of ubiquitin-taggedproteins [41]. Ubiquitination of proteins is catalysed bythree enzymes: ubiquitin-activating enzyme (E1),ubiquitin-conjugating enzyme (E2) and ubiquitin ... starvation the rate of protein synthesis is limited by the size of the free amino acid pool and that autophagy isrequired to provide the cell with amino acids for newprotein synthesis [26]. During ... proteins, there are two main scenarios.Either, some proteins are hidden from the protein-degradation machinery or this machinery recognizes the different proteins and distinguishes between them.There...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... This is in agreement with aprevious study in which the synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positiveeffect on the activity of the other enzymes involved ... proteins in A. niger [33]. The fusion construct (pIM3982) was made by introducingan NsiI site at the translation start point of galA and by thencloning a fragment, starting at this NsiI site and ... them, are the trueinducers of galA expression. This indicates that galA iscoexpressed with both the pectinolytic genes encoding mainchain cleaving enzymes as well as with the arabinanolyticgenes...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... CTPsynthase was performed using% Inhibition ¼ 100% I nKn I þ I nð7Þwhere K I is the concentration of inhibitor that gives rise to50% inhibition by the inhibitor I and n is the Hill-coefficient. ... Steady-state kinetics of the glutaminase reaction of CTP synthasefromLactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesisMartin ... regulation; Lactococcus lactis.CTP synthase (EC 6.4.3.2) catalyzes the synthesis of CTPfrom UTP by amination of the pyrimidine ring at the 4-position. The enzyme has three functionally distinct sites;the...
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Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
... explored the possibility of modifying the psychrophilic properties of an enzyme byintroducing, via mutagenesis, predictable flexibility changesto key active site residues of the psychrophilic alkalinephosphatase ... substitution of Tyr269 fiAla was designed with the aim of reducing the spatialconstraints originating from the side-chain interactions between Tyr269 and Ala261 (Fig. 2C). The main difference between the ... fullpotential of protein engineering in the redesign of psychro-philic enzyme properties [15]. In particular, the effects of local flexibility in psychrophilic enzyme properties havebeen so far studied...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... concen-trations of this compound, the incorporation of 14C-labelfrom the cyclic diphosphate into the lipid-soluble fractionwas significantly diminished. In a similar experiment, we studied the formation of ... biosynthesis in plants (Eur. J. Biochem. 268) 6307into phytoene (the main labeled component of the lipid-soluble fraction) is systematically diminished by the addition of increasing amounts of [1-3H]2C-methyl-D-ery-thritol ... aspseudotriplets that could be due to long range couplinginvolving vicinal isoprenoid moieties. Due to the linebroadening, the precision of signal integration is substan-tially reduced. However, within...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... endotoxin comprise the initiating event lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, we employed GdCl3to inactivate ... cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform activities and gene expression profiles, partly by investigating the ... oxidative stress contrib-utes to the inhibition of CYP activity observed in the absence of changes in protein expression in rabbit he-patocytes [22]. ROS indirectly reduced the activity of selected...
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