... ortholog of HRPC2 (91% amino
acid identity). Shinmyo et al. [64] have studied the promoter
activity and wound-induction of HRPC and -E types, and
of AtPCa and AtPEa. Most remarkably, C2, and only C2,
responded ... Differential
activity and structure of highly similar peroxidases. Spectroscopic,
crystallographic, and enzymatic analyses of lignifying Arabidopsis
thali...
... GRRPsLFG
and APDyTTPEMY-NH
2
were obtained from Dr A
˚
ke
Engstro
¨
m at the Peptide Synthesis and Analysis Laboratory
of the Department of Medical Biochemistry and Microbio-
logy, Uppsala University. The ... obtained from Promega, and LysC of
Achromobacter lytii from Wako. The O-phosphorylated
phosphopeptides EQTRsLDGR-NH
2
, DHTGFLtEY-
VATRWC-NH
2
, DADEyL-NH
2
, EGDNDyI...
... reverse of
synthesis is by a different mechanism from base exchange
[20]. Further analysis of the products released from PtdIns
by the Arabidopsis enzyme will allow us to carry out a
deeper study of ... Phosphatidylinositol synthesis and exchange of the inositol head
are catalysed by the single phosphatidylinositol synthase 1
from
Arabidopsis
Anne-Marie Justin, Jean-Claude...
... com-
plexes may represent true physiologically relevant
interactions in stationarily grown cells.
Identification of proteins X (GatY) and Y (UP12)
In order to identify polypeptides X and Y, we repeated ... of ybdQ deletion on cell growth at 37 °C and on sensitivity towards CCCP exposure. (A) Growth curves of wild-type and D ybdQ cells in
Luria–Bertani broth. Single colonies o...
... Molecular Biology, University of Debrecen, Hungary;
3
Department of Inorganic Chemistry,
and
4
Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary;
5
International ... Ko¨ve
´
r
3
, Gyula Batta
4
and Jacek Kuz
´
nicki
1,5
1
Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland;
2
Depar...
... alignment of the
interdomain and autolysis loops of
mammalian chymotrypsins and trypsins.
The enzyme that was used in this study, rat
chymotrypsin B, is highlighted by bold type.
The loops are boxed and ... that of
D-chymotrypsin and Tyr146!His/Asn147!Ser D-chymo-
trypsin remained the same. In a second set of experiments, a
Fig. 3. Chymotryptic degradation of d-chymotrypsino...
... between His230 of
PP2A-C and Asp119 and Asn94 of Anp32a. A salt
bridge could form between Glu226 of PP2A-C and
Lys67, Lys68 and Lys91.
Testing the interaction with Atx1 experimentally
Interaction ... The
identification of phosphorylation sites of pp32 and bio-
chemical purification of a cellular pp32-kinase. Biochem-
istry 43, 10157–10165.
27 Bairoch A (1992) PROSITE: a di...
... model of the P-pyridoxyl-E. coli PEP carboxykinase
adduct. N
e
from Lys288 of the open structure of E. coli PEP carb-
oxykinase (1OEN) is covalently linked to the carbonyl carbon of the
P-pyridoxyl ... Asp269, and N
e
from Lys213.
Ó FEBS 2002 Ligand interactions of PEP carboxykinase (Eur. J. Biochem. 269) 4961
N
e
from Lys288 of the open structure was covalently linked
to...
... production of cystatin F compared
to the ubiquitous inhibitor, cystatin C, we measured the
cystatin contents in cell lysates and culture media of cells
grown for 2 and 4 days with cystatin F and cystatin ... exists in these two glycosylated
forms, but also as one most likely unglycosylated species
(lane 3, band C), with mobility exactly as recombinant
cystatin F after deglycosylatio...
... standardization of each synthetic peptide was
achieved by acid hydrolysis of a known gravimetric quantity
of lyophilisate followed by amino acid analysis using an
Applied Biosystems PTH-amino acid analyser.
cDNA ... degree of primary structural variability of
bradykinins in amphibian skin secretions, their differing
spectra of bioactivity and the lack of information on
brady...