Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

... ortholog of HRPC2 (91% amino acid identity). Shinmyo et al. [64] have studied the promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2, responded ... Differential activity and structure of highly similar peroxidases. Spectroscopic, crystallographic, and enzymatic analyses of lignifying Arabidopsis thali...

Ngày tải lên: 21/02/2014, 01:21

... GRRPsLFG and APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The ... obtained from Promega, and LysC of Achromobacter lytii from Wako. The O-phosphorylated phosphopeptides EQTRsLDGR-NH 2 , DHTGFLtEY- VATRWC-NH 2 , DADEyL-NH 2 , EGDNDyI...

Ngày tải lên: 21/02/2014, 01:21

... reverse of synthesis is by a different mechanism from base exchange [20]. Further analysis of the products released from PtdIns by the Arabidopsis enzyme will allow us to carry out a deeper study of ... Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis Anne-Marie Justin, Jean-Claude...

Ngày tải lên: 22/02/2014, 04:20

... com- plexes may represent true physiologically relevant interactions in stationarily grown cells. Identification of proteins X (GatY) and Y (UP12) In order to identify polypeptides X and Y, we repeated ... of ybdQ deletion on cell growth at 37 °C and on sensitivity towards CCCP exposure. (A) Growth curves of wild-type and D ybdQ cells in Luria–Bertani broth. Single colonies o...

Ngày tải lên: 22/02/2014, 07:20

... Molecular Biology, University of Debrecen, Hungary; 3 Department of Inorganic Chemistry, and 4 Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary; 5 International ... Ko¨ve ´ r 3 , Gyula Batta 4 and Jacek Kuz ´ nicki 1,5 1 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland; 2 Depar...

Ngày tải lên: 22/02/2014, 07:20

... alignment of the interdomain and autolysis loops of mammalian chymotrypsins and trypsins. The enzyme that was used in this study, rat chymotrypsin B, is highlighted by bold type. The loops are boxed and ... that of D-chymotrypsin and Tyr146!His/Asn147!Ser D-chymo- trypsin remained the same. In a second set of experiments, a Fig. 3. Chymotryptic degradation of d-chymotrypsino...

Ngày tải lên: 22/02/2014, 07:20

... between His230 of PP2A-C and Asp119 and Asn94 of Anp32a. A salt bridge could form between Glu226 of PP2A-C and Lys67, Lys68 and Lys91. Testing the interaction with Atx1 experimentally Interaction ... The identification of phosphorylation sites of pp32 and bio- chemical purification of a cellular pp32-kinase. Biochem- istry 43, 10157–10165. 27 Bairoch A (1992) PROSITE: a di...

Ngày tải lên: 18/02/2014, 17:20

... model of the P-pyridoxyl-E. coli PEP carboxykinase adduct. N e from Lys288 of the open structure of E. coli PEP carb- oxykinase (1OEN) is covalently linked to the carbonyl carbon of the P-pyridoxyl ... Asp269, and N e from Lys213. Ó FEBS 2002 Ligand interactions of PEP carboxykinase (Eur. J. Biochem. 269) 4961 N e from Lys288 of the open structure was covalently linked to...

Ngày tải lên: 21/02/2014, 01:21

... production of cystatin F compared to the ubiquitous inhibitor, cystatin C, we measured the cystatin contents in cell lysates and culture media of cells grown for 2 and 4 days with cystatin F and cystatin ... exists in these two glycosylated forms, but also as one most likely unglycosylated species (lane 3, band C), with mobility exactly as recombinant cystatin F after deglycosylatio...

Ngày tải lên: 21/02/2014, 01:21

... standardization of each synthetic peptide was achieved by acid hydrolysis of a known gravimetric quantity of lyophilisate followed by amino acid analysis using an Applied Biosystems PTH-amino acid analyser. cDNA ... degree of primary structural variability of bradykinins in amphibian skin secretions, their differing spectra of bioactivity and the lack of information on brady...

Ngày tải lên: 21/02/2014, 15:20