Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected by hand. Since the sequences of the two arginases and agmatinase greately differ in the ... in the case of E. coli agmatinase, of the loop indicated by the letter a.Fig. 5. Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the ... Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling A critical role for aspartate 153Mo´nica Salas1, Rolando Rodrı´guez2,...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... spectroscopy [17].To compar e the mechanism of h ydrolysis of IPU and o therGH family 49 dextranases, the a nomer configuration of the hydrolysate of IPU was determined. A polarimetric assaywas ... respectively, were used. The proteinconcentration was measured by the method of Lowryet al. with B SA as standard [37].TLCTLC was p erformed to analyse t he hydrolysates of the W402F enzyme. The reaction ... conservedregions of all the GH family 49 enzymes (Fig. 1). Todetermine the catalytic r esidues of I PU, these Asp a nd Gluresidues are replaced by Asn and Gln, respectively. Five of these enzymes (E273Q,...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... soybean [18], cowpea[19], and poppy [20,21].In the present study, the first insights into the secon-dary and tertiary structure of PLD a2 from cabbageare given. The surface structure of the ... effect by calcium ions.Destabilization of PLDa2 by calcium ions was alsoindicated by the proteolytic sensitivity of PLD a2 [34].Calcium-induced decrease of stability and biologicalactivity due ... illustrates the quality of the experimental data(Fig. 2B).Reconstruction of the overall shape of the PLDa2from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... Insights into the interaction of human arginase II withsubstrate and manganese ions by site-directed mutagenesis and kinetic studiesAlteration of substrate specificity by replacement of Asn149with ... molecule, by lowering the pKafor proton ionization and generation of the hydroxide that nucleophilically attacks the guanidinocarbon of the scissile bond of l-arginine [1–3]. The enzyme is widely ... analysis of the wild-type and His145Asn variants. In addition to removal of the more weakly bound Mn2+, the lower affinity for thatmore tightly bound to the protein may explain the absence of...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... exchange of the HA-hydrogen of IPP. Inboth cases, scrambling of the label takes place within the IPPpool and the error is then transcribed into the DMAPP pool by the normal action of the isomerase. ... exchange (2% of the isomerization rate) of the methyl group of IPP and the (Z)-methyl group of DMAPP, and an even slower exchange(0.5% of the isomerization rate) of the olefinic hydrogen of DMAPP corresponding ... in E. coli 2C-methyl-D-erythritol4-phosphate can be converted into a cyclic diphosphate by the consecutive action of 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, 4-diphosphocytidyl-2C-methyl-D-erythritolkinase...
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Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity pdf
... fact, the bulk of the proteolytic processing of the proforms of the SVMPs occurs in advance of release of mature secre-tory vesicle contents into the lumen of the venom gland.In the case of the ... 3017proteins are synthesized in the cytoplasm of secretorycells in the gland and transferred to the rough endo-plasmic reticulum, and then the Golgi apparatus, and finally transported via secretory granules ... be the evolutionary result of the loss of the cysteinyl residuein this region, thereby structurally freeing the loop forintegrin interaction, coupled with the presence of the integrin-binding...
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Tài liệu Báo cáo khoa học: "Insights into Non-projectivity in Hindi" docx
... at the rigidity of these non-projectiveconstructions and their best projective approxi-mation possible by reordering. Rigidity here is the reorderability of the constructions retaining the ... inHyDT, by definition, represents a set of adjacentwords which are in dependency relation with eachother, and are connected to the rest of the words by a single incoming dependency arc. The rela-tions ... in HyDT.We also analyse non-projectivity in Hindiin terms of various linguistic parameterssuch as the causes of non-projectivity,its rigidity (possibility of reordering) and whether the reordered...
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot
... . The X-ray crystal structure 1 pi2 reveals that the /,w angles of most of the residues of the Bowman±Birk inhibitor aredistributed fairly evenly over the b- and PPII-regions of the Ramachandran ... strands, they would be expected to readily undergo thistype of aggregation w ith each other and a lso with the edges of established bsheet. The m ore d ynamic type of disorderassociated with the ... ases of Parkinson's disease.Unfortunately the quality of s ome of these syn uclein ROAspectra i s generally not as good as that of the caseins d ue inpart to the high concentrations of...
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Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot
... pathways from the synthesis of 3-ketoacylCoAs by a condensing enzyme to the incorporation of fattyacids into lipids such as triacylglycerol or membranephospholipids. This in vivo system is therefore ... Elonga-tion of [1-14C]18:1CoA to [3-14C]20:1CoA and [5-14C]22:1CoA by the microsomes was measured by incubation with malonyl CoA and required cofactors,followed by transacylation of the CoA ... monounsaturatedfatty acids were elongated by the FAE1 KCSs to a greaterdegree than were saturated fatty acids. This may reflect the available substrates in this yeast system and/ or the ability of the enzymes...
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Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx
... hypotheticalsecond site have been made here for the bacterial enzyme by stripping subunits III and IV off the native complex, and by further destroying a large part of the acidic lobe(s) of the docking ... complex of the respiratory chains of mitochondria and many bacteria[1–4]. It catalyzes the reduction of oxygen to water, coupling the free energy of this reaction to the generation of a protongradient ... W121Qactivity, i.e. its (already low) residual electron transferactivity is not maintained by the neighbouring tyrosine,while the W121F /Y1 22F mutant activity may be viewed asa commitment of the tyrosine...
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Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdftài liệu báo cáo nghiên cứu khoa họctai lieu bao cao thuc tap y si da khoatai lieu bao cao thuc tap tim hieu nhan cach mot hoc sinhbáo cáo y họctài liệu báo cáotài liệu báo cáo môn triếttài liệu báo cáo tài chínhtài liệu di truyền y họctài liệu báo cáo mônbáo cáo y họctài liệu báo cáo tài chính vốn bằng tiền tai doanh nghiệptài liệu vi sinh y họctài liệu báo cáo môn triếtquan hệ sản xuấttài liệu báo cáo thuếNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ