Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected by hand. Since the sequences of the two arginases and agmatinase greately differ in the ... in the case of E. coli agmatinase, of the loop indicated by the letter a. Fig. 5. Scheme of the binuclear manganese cluster and the localization...

Ngày tải lên: 21/02/2014, 01:21

... spectroscopy [17]. To compar e the mechanism of h ydrolysis of IPU and o ther GH family 49 dextranases, the a nomer configuration of the hydrolysate of IPU was determined. A polarimetric assay was ... respectively, were used. The protein concentration was measured by the method of Lowry et al. with B SA as standard [37]. TLC TLC was p erformed to analyse t he hydrolys...

Ngày tải lên: 19/02/2014, 16:20

... soybean [18], cowpea [19], and poppy [20,21]. In the present study, the first insights into the secon- dary and tertiary structure of PLD a2 from cabbage are given. The surface structure of the ... effect by calcium ions. Destabilization of PLDa2 by calcium ions was also indicated by the proteolytic sensitivity of PLD a2 [34]. Calcium-induced decrease of sta...

Ngày tải lên: 19/02/2014, 02:20

... Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with ... molecule, by lowering the pK a for proton ionization and generation of the hydroxide that nucleophilically attacks the guanidino carbon of the scissile...

Ngày tải lên: 20/02/2014, 02:21

... exchange of the H A -hydrogen of IPP. In both cases, scrambling of the label takes place within the IPP pool and the error is then transcribed into the DMAPP pool by the normal action of the isomerase. ... exchange (2% of the isomerization rate) of the methyl group of IPP and the (Z)-methyl group of DMAPP, and an even slower exchange (0.5% of t...

Ngày tải lên: 22/02/2014, 07:20

... fact, the bulk of the proteolytic processing of the proforms of the SVMPs occurs in advance of release of mature secre- tory vesicle contents into the lumen of the venom gland. In the case of the ... 3017 proteins are synthesized in the cytoplasm of secretory cells in the gland and transferred to the rough endo- plasmic reticulum, and then the Gol...

Ngày tải lên: 18/02/2014, 18:20

... at the rigidity of these non-projective constructions and their best projective approxi- mation possible by reordering. Rigidity here is the reorderability of the constructions retaining the ... in HyDT, by definition, represents a set of adjacent words which are in dependency relation with each other, and are connected to the rest of the words by a single incomin...

Ngày tải lên: 20/02/2014, 09:20

... . The X-ray crystal structure 1 pi2 reveals that the /,w angles of most of the residues of the Bowman±Birk inhibitor are distributed fairly evenly over the b- and PPII-regions of the Ramachandran ... strands, they would be expected to readily undergo this type of aggregation w ith each other and a lso with the edges of established bsheet. The m ore d ynamic typ...

Ngày tải lên: 21/02/2014, 15:20

... pathways from the synthesis of 3-ketoacyl CoAs by a condensing enzyme to the incorporation of fatty acids into lipids such as triacylglycerol or membrane phospholipids. This in vivo system is therefore ... Elonga- tion of [1- 14 C]18:1CoA to [3- 14 C]20:1CoA and [5- 14 C]22:1CoA by the microsomes was measured by incubation with malonyl CoA and required cofactors, follo...

Ngày tải lên: 21/02/2014, 15:20

... hypothetical second site have been made here for the bacterial enzyme by stripping subunits III and IV off the native complex, and by further destroying a large part of the acidic lobe(s) of the docking ... complex of the respiratory chains of mitochondria and many bacteria [1–4]. It catalyzes the reduction of oxygen to water, coupling the free energy of th...

Ngày tải lên: 22/02/2014, 07:20