Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

... displayed significant glutathione- dependent dehydroascorbate reductase and thiol transferase enzymatic activities. Keywords: glutathione S -transferase; dehydroascorbate reductase; thiol transferase; ... Schistosoma mansoni (Eur. J. Biochem. 269) 5521 Characterization of an omega-class glutathione S -transferase from Schistosoma mansoni with glu...

Ngày tải lên: 21/02/2014, 01:21

... Eggertsson 1 and Magnu ´ s M. Kristja ´ nsson 2 1 Institute of Biology, University of Iceland; and 2 Department of Biochemistry, Science Institute, University of Iceland, Reykjavik, Iceland The gene ... elements based on known crystal structures of PRK are indicated by h (helix) and s (strand) and calcium binding ligands (P175, V177 and D200 at the Ca1 site and T16 and...

Ngày tải lên: 21/02/2014, 01:21

... characterization of a novel B. mori UDP-glucosyltransferase gene, Bmugt1 ,and analysis of the activity of the enzyme towards a range of Correspondence to D. R. O’Reilly, Department of Biology, Imperial College ... amino-acid identity overall w ith other members of the family, e.g. 31% and 38% with Drosophila melano gaster UGT35a and UGT35b [12], respectively, and 30% w...

Ngày tải lên: 22/02/2014, 04:20

... Electrophoretic analysis and purification of recombinant ASP3c. (A) SDS/PAGE analysis of recombinant ASP3c secreted by Pichia pastoris. Lane 1 shows standards (Low range and Polypeptide kits, Bio-Rad, France) ... digested by trypsin and the resulting peptides were separated by RPLC as described by Briand et al.[48].The fractions were manually collected. N-Terminal amino acid sequence...

Ngày tải lên: 21/02/2014, 03:20

... lack of an error bar indicates an SD smaller than the size of the symbols. Table 1. Effect of different divalent cations on cytosolic and membrane- bound PtdEtn-PLD activity. Cytosolic and membrane-bound ... a transphosphatidylation with primary short-chain alcohols. We have characterized the cytosolic and mem- brane-bound forms of the yeast PtdEtn-PLD and examined the reg...

Ngày tải lên: 22/02/2014, 07:20

... PC and increased GPC, indicating an imbal- ance between PL synthesis and degradation and, con- sequently, an increase of TG. Other studies suggest that the simultaneous accumulation of ML and ... 1.22 and 1.40 p.p.m. (Table 1 and Figs 2 and 3). These signals most likely arise from the aggregation of large molecules because they are characterized by large linewidths (T...

Ngày tải lên: 18/02/2014, 13:20

... of the Argenton IFREMER experimental hatchery for the production of oyster embryos and larvae. The authors thank Christophe Fleury and Emeline Furon (University of Caen) for technical assistance. References 1 ... sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden- tity) argues for a conser...

Ngày tải lên: 19/02/2014, 00:20

... by the arrow. Oligonucleotides Sp1 and mSp1 con- tained the wild and mutant types of Sp1 site, respectively (lanes 4 and 5). The nuclear extract was preincubated with anti-PDX-1 (lane 8) or anti-Sp1 ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan 2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan 3 Division of Legal...

Ngày tải lên: 19/02/2014, 06:20

... fam- ily of plant regulatory proteins, as at least five A. thali- ana genes and many expressed sequence tags (ESTs) from different plant species encode proteins with signi- ficant similarity to PPI1 ... alignments and intron positions [3,4]. Individual isoforms exhibit tissue- and developmental-specific expression patterns and a number of quantitative differences in catalytic an...

Ngày tải lên: 19/02/2014, 07:20

... optimum with > 90% activity in the temperature range 40–70 °C. Effect of SDS and EDTA on activity The effect of SDS on activity of SPRK and PRK was measured by addition of 0.1, 0.25, 0.5 and 1.0% ... dCTP, dGTP and dTTP, 0.2 lm gene specific primer and adaptor primer and 1 U Pfu-poly- merase (Promega) and 94 °C for 2 min, 30 cycles of 94 °C for 30 s, 55 °C for 1 mi...

Ngày tải lên: 19/02/2014, 07:20