Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

... Spain Novel properties of the primer independent synthesis of poly(A), catalyzed by the yeast poly(A) polymerase are presented. The commercial enzyme from yeast, in contrast to theenzymefromEscherichia ... independent synthesis of poly(A) In order to understand why dinucleoside polyphosphates activated the primer independent synthesis of poly...

Ngày tải lên: 21/02/2014, 01:21

... to that of free VIIa–TF, which is also an evidence of the hypothesis. Fig. 5. Verification of the hypotheses for TFPI inhibitory mechanism. (A) Verification of the hypothesis of the enzyme–product ... values of the hypothetical reactions separately. The model of the inhibitory action of the Xa–TFPI complex on the enzyme–substrate complex (model 3) The reactio...

Ngày tải lên: 22/02/2014, 04:20

... Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science, Tokyo, Japan; 2 Yoneyama Maternity Hospital, Tokyo, Japan Previously, we demonstrated apoptotic cell death in the chorion ... these cytokines in apoptosis may already be complete at the time the fetal membrane tissues were prepared, or that the role of these cytokines may be connected indirectly...

Ngày tải lên: 22/02/2014, 07:20

... 8C. Then 0.1 vol. of a solution of 0.5 M sodium acetate (pH 5.0) was added and the activity of the enzyme was measured by the dinitrosalicylic acid method. Inhibition of the enzyme activity by ... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, the active site of the C. sepium b-amylase most probably consists of a...

Ngày tải lên: 22/02/2014, 07:20

... production of yolk protein precursors (YPPs) by the fat body and their subsequent internalization into the developing oocyte, helping to support later embryonic development. The two primary insect Keywords ecdysone ... regulators in the synthesis of vitellogenin (Vg), the main YPP protein produced by the fat body [4,5]. The molecular mechanism of 20E action has been...

Ngày tải lên: 18/02/2014, 13:20

... eu- karyotes, but is absent in prokaryotes and yeast. Poly(ADP-ribosyl)ation is catalysed by the family of poly(ADP-ribose) polymerases (PARPs; Fig. 2), enco- ded in human cells by a set of 18 ... linkages. The sites of hydrolysis catalysed by poly(ADP-ribose) glycohydrolase (PARG), the major poly(ADP- ribose)-degrading enzyme, are indicated by arrows. A. Bu ¨ rkle Poly(...

Ngày tải lên: 20/02/2014, 01:20

... crystals were grown and flash-cooled to 100 K under anaerobic conditions. The redox state of the PETN crystals could easily by assessed and verified by bright yellow colour of the oxidized crystals vs. ... not grossly perturb the overall framework of the enzyme or the active site. The active sites of the enzymes are shown in stick format with the sigmaA weigh- ted...

Ngày tải lên: 20/02/2014, 02:21

... that the dimeric structure of the enzyme is essential for its display of cytotoxic activity [3]. This conclusion was based on the lack of cytotoxic activity in a monomeric derivative of the protein ... obtained by selective reduction of the protein intersubunit disulfides followed by alkylation of the exposed sulfhydryls with iodoacetate, iodoacetamide or 2-bromoeth...

Ngày tải lên: 20/02/2014, 11:20

... phosphoenolpyruvate (PEP) carboxykinase catalyzes the decarboxylation of oxaloacetate and transfer of the c-phosphoryl group of ATP to yield PEP, ADP, and CO 2 . The interaction of the enzyme with the substrates ... another substrate of the enzyme, also increased the P-pyridoxyl fluorescence. The addition of this substrate to the protein blue shifted the maximum...

Ngày tải lên: 21/02/2014, 01:21

... stereoselective synthesis of enantiomerically pure mono- and di-O-hexadecyl-b- D -glucosylglycerol ethers by epoxidation of an allyl b- D -glucopyranoside asymmetrically induced by the glucide moiety. Tetrahedron: ... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxid...

Ngày tải lên: 21/02/2014, 01:21