Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phospho enol pyruvate carboxykinase determined by fluorescence spectroscopy Marı ´ a ... solution. Keywords: Escherichia coli phosphoenolpyruvate carboxy- kinase; ligand binding; conformational changes; P-pyridoxyl fluorescence spectroscopy. Escher...

Ngày tải lên: 21/02/2014, 01:21

... dephosphorylation of the phosphopeptide by porcine liver cytosol was apparently not due to protein phos- phatases 1, 2A and 2C as judged from the lack of inhibition by okadaic acid and EDTA. ... at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ a...

Ngày tải lên: 21/02/2014, 01:21

... washed and mounted as above. Quantitative protein assays Cell lysates and culture media were analysed by ELISA to determine the concentrations of cystatin F [5] and cystatin C [14,15]. The total protein ... production of cystatin F compared to the ubiquitous inhibitor, cystatin C, we measured the cystatin contents in cell lysates and culture media of cells grown for 2 and...

Ngày tải lên: 21/02/2014, 01:21

... ortholog of HRPC2 (91% amino acid identity). Shinmyo et al. [64] have studied the promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2, responded ... Differential activity and structure of highly similar peroxidases. Spectroscopic, crystallographic, and enzymatic analyses of lignifying Arabidopsis thaliana...

Ngày tải lên: 21/02/2014, 01:21

... The Ikaros family protein Eos associates with C-terminal-binding protein corepressors Jose ´ Perdomo and Merlin Crossley Department of Biochemistry, G08, University of Sydney, NSW, Australia Eos ... cloning of Aiolos and Helios [6–8], and of Eos and Pegasus [9], revealed the existence of a family of related factors. Ikaros, Aiolos and Helios are all abundantly expres...

Ngày tải lên: 21/02/2014, 01:21

... methods. Enzyme activity was a nalysed by the standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials and methods. Open and filled ... ensitometer scanning of a Western b lot of cell lysates, t he enzyme activity increased only threefold (data not shown). Investi- gation of the cell lysate by gel filtration de...

Ngày tải lên: 21/02/2014, 15:20

... potenti- ation of the effects of endogenous bradykinin by inhibition of catabolic proteases (snakes and scorpions) or by local delivery of supraphysiological quantities of bradykinins (wasps and amphibians). Although ... standardization of each synthetic peptide was achieved by acid hydrolysis of a known gravimetric quantity of lyophilisate followed by amino acid a...

Ngày tải lên: 21/02/2014, 15:20

... Wajant 1 1 Institute of Cell Biology and Immunology and 2 Institute of Industrial Genetics, University of Stuttgart, Germany; 3 Institute of Immunology, Christian-Albrechts-University of Kiel, Germany To gain ... 8). Correspondingly, fluorescence of cytoplasmic T4(259–470)– YFP and full-length T4–GFP was already significantly reduced after 2 min of bleaching in a small...

Ngày tải lên: 21/02/2014, 15:20

... Bal 1,2 1 Faculty of Chemistry, University of Wroclaw, Poland; 2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland A comparative study of thermodynamic and kinetic ... pooled and lyophilized to yield with solid of purity exceeding 99% as assessed by HPLC analysis of the final m aterials. I dentity and purity of peptide was confirmed...

Ngày tải lên: 22/02/2014, 04:20

... novo synthesis, catalysed by phosphatidylinositol synthase (EC 2.7.8.11), also known as CDP-diacylglycerol (DAG)/myo- inositol 3-phosphatidyltransferase, and an exchange reac- tion whereby the ... reverse of synthesis is by a different mechanism from base exchange [20]. Further analysis of the products released from PtdIns by the Arabidopsis enzyme will allow us to carry out a dee...

Ngày tải lên: 22/02/2014, 04:20