Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc

... Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli Stjepan Orhanovic ´ and Maja Pavela-Vranc ˇ ic ˇ Department of Chemistry, Faculty of Natural Sciences, ... catalysis of both subunits. Model representation of the catalytic cycle for APase from E. coli A model describing the catalytic mechanism of APase from...

Ngày tải lên: 21/02/2014, 00:20

... that the word pair consisting of the left and right neighbor of a particular token is characteristic of the part of speech at this position, and by clustering the neighbor pairs on the basis ... be sur- rounded by the pair the is”, a verb by the pair “he the , and an adjective by the pair the thing”. For ease of reference, in the remainder of this...

Ngày tải lên: 20/02/2014, 12:20

... the condensation and fragmentation of their nuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... lost. Taken together, our results demonstrate an impor- tant relationship among the regulation of nucleo- lin ⁄ C23, the activation of caspase-3 and the induction of...

Ngày tải lên: 16/02/2014, 09:20

... being the most studied of these. IFs maintain cell shape and the structural integrity of cell contents, and provide protection against various types of stress. The mechanism of action of OA as ... metabolism and coor- dination of the cell cycle and gene expression. The crit- ical importance of phosphatases in cell metabolism is underlined by the fact that t...

Ngày tải lên: 18/02/2014, 14:20

... palytoxin action against the actin cytoskeleton (Fig. 3). At present, in spite of the impor- tance of the unpolymerized actin pool for the mainte- nance of the F-actin system, the cellular response Fig. ... constitute the actin cytoskeleton [13]. For a long time, it was believed that the role of this system was mainly structural, providing the sup- port needed to mai...

Ngày tải lên: 18/02/2014, 14:20

... been isolated from the digestive glands of scallops collected in Japan, and these are stereoisomers of PTX-1 and PTX-6, respectively. After acid treatment of PTX-4 and PTX-7, two isomers, named PTX-8 and ... supports the idea that lactone ring integrity is essential for the action of PTXs; oxidation on C43 decrease the toxic- ity of the molecule, and oxidation...

Ngày tải lên: 18/02/2014, 14:20

... standard light ⁄ dark cycle (lights on at 07:00 h). The experiments were performed according to the Guide for the Care and Use of Laboratory Animals of the US Department of Health and Human Services, ... measured in the control (n ¼ 9) and pilocarpine (n ¼ 8) groups by the method that uses H 2 O 2 to generate H 2 O and O 2 [36]. The activity was measured by the...

Ngày tải lên: 19/02/2014, 16:20

... GdmCl on the secondary structure of the protein. In the far-UV region, the CD spectra of the FprA show the presence of substantial a-helical conformation [15]. Fig. 1C sum- marizes the effect of increasing ... C-terminal regions of the enzyme, and the NADPH-binding domain (residues 109–323) consisting of the central part of the polypeptide chain [8]. A sm...

Ngày tải lên: 19/02/2014, 16:20

... man [8,9]. In the verteb- rate lens, the b and c crystallins together account for the majority of the soluble proteins (the other major family being the a-crystallins, members of the small heat-shock ... to others, but is essen- tially an outlier from both b and c families. Overall, these data show a broader and more com- plex view of the evolution of the bc s...

Ngày tải lên: 19/02/2014, 17:20

... ATnI 130)252 , and ATnI 232)292 did not migrate into the gels, while free ATnI-52K and ATnI 1)128 exhibited a band near the origin and at the middle of the gel, respectively. The bands corresponding to the ... understand the biological significance of the differ- ence in TnI–TnC interactions, we compared the ability of TnC to neutralize the inhibitory effects o...

Ngày tải lên: 20/02/2014, 01:20