Tài liệu Báo cáo khoa học: Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efficiency and Ca2+-sensitivity ppt

... Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efficiency and Ca 2+ -sensitivity Ji-Young Hwang 1, *, Christian Lange 1, †, Andreas Helten 1 , ... bovine and amphibian rod outer segments [6–9], and it is the only cyclase which has been cloned based on its amino acid sequence [8,10]. Human retinal diseases (LCA1 and C...

Ngày tải lên: 21/02/2014, 00:20

... N-terminus (Met1) of Tat(1–9) and Asp709 as well as Asp739 of DP IV, between the C-terminal Glu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of DP IV. Furthermore, the side chain of Asp5 of ... suitable for the determination of the K i value. From the Hill-plot, binding of the inhibitor to different binding sites was reflected by the change of the slope in...

Ngày tải lên: 20/02/2014, 11:20

... CGM and G CX + ⁄ CGM), 5-methylcytosine was introduced into one of the strands of the recognition site instead of the target cytosine. The melting curves of the hemi- methylated X + CG ⁄ CGM and ... DNA and AdoHcy(AdoMet) [44], M.HhaI consists of two domains, the large domain containing the S-adenosyl-l-methionine (AdoMet) binding site and the catalytic center, and the s...

Ngày tải lên: 19/02/2014, 00:20

... staining of 293-VnR and ATDC5 cells expressing the Y373C mutant with anti- FGFR3 and anti-phosphotyrosine sera showed both intracellular and cell-surface phosphotyrosine staining (Figs 2Eb,c and ... 70Z-Cbl (a mutant lacking 17 amino acids in the linker and RING finger domain of c-Cbl) and wild-type or mutant FGFR3 cDNAs as indicated, then immunoprecipitated and blotted as...

Ngày tải lên: 19/02/2014, 00:20

... channeling Prior investigations of channeling may be divided into two categories: in vitro and in vivo. The former include experiments with cell extracts and purified enzymes. These are inherently ... and 3 H-labeled protein were measured. The 3 Hin protein was insignificant, showing that the aminoacyl- tRNA made from amino acids and tRNA did not mix with the introduced aminoacyl-...

Ngày tải lên: 19/02/2014, 18:20

... channels. The RSVP interface is of particular utility for the most impaired users, including those suffering from locked -in syndrome (LIS). Locked -in syndrome can result from traumatic brain injury, such ... seen in the timing diagram shown in Figure 5, epoch k contains between 1 and M k sequences. This figure shows the onset of each se- quence, each fixation image (which is shown...

Ngày tải lên: 20/02/2014, 05:20

... study of the role of recombination in mtDNA inheritance in S. cerevisiae showed that budding cells were enriched in linear monomers of mtDNA. It was proposed that in addition to a role in initiating ... species. The role of Ydc2 for maintaining the content of mtDNA in fission yeast is not clear. Apart from DNA repair, recombination could be involved in the initiation o...

Ngày tải lên: 20/02/2014, 11:20

... inserted into the ACT2 domain; and (c) two ACT domains, each from a different chain, interact to form an effec- tor-binding unit. The effector-binding unit of the ACT domain in the b-subunit of CgAK ... 53–59 in chain B, residues 55–56 in chain C, residues 56–57 in chain E, and residues 53–56 in chain F) of the loop between b3 and b4 are not observed in every chain. Th...

Ngày tải lên: 18/02/2014, 08:20

... as being some combination of the following: (a) production of redox potential in the form of NADPH; (b) production of glycolytic intermediates, reducing the demand put upon phosphofructokinase; ... be defined in terms of modes thus: a mode of a system is a set of reactions whose net stoichiometry (i.e. in terms of external substrates and products) is balanced and wi...

Ngày tải lên: 20/02/2014, 23:20

... accompanied by improved bind- ing to longer ligands (up to five atoms in the main chain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 from native GLYAT ... thermo- stability and protein expression in E. coli determined for wild-type GO and variants of GO obtained by site-saturation mutagenesis of the positions identified by docking a...

Ngày tải lên: 14/02/2014, 14:20