Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf
Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf
... Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies Alessio Cardinale, Ilaria Filesi, Sonia Mattei and Silvia ... Italy Anti-Ras intracellular antibodies inhibit cell proliferation in vivo by sequestering the antigen and diverting it from itsphysiological location [Lener, M., Horn, I. R., Cardinale, A.,Messina, ... specific proteasome inhibitor lactacystin [27] were analysed by Western blotting. As can be seen in Fig. 3A, accumulationof the 32-kDa protein, corresponding to the single-chainaD11-sec, was induced...
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Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt
... tetra-thiolate sites, the choice for the third incoming metalion would be to form a bridging interaction in thea domain or to form another independent tetrathiolatesite in the b domain. It is probable that ... to coordinate tothe two-domain ba-MT in a domain-specific manner,with a preference for the a domain, has been construedas being an indicator of cooperative metal binding tothe a domain. This ... con-stants for the a domain are greater than those for theb domain may be a factor in explaining the observedmetal ion distribution. After coordination of the firsttwo metal ions to the a domain in independent...
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Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx
... anessential twin-arginine motif in the N-terminal domain[9,10]. They then interact with a translocon in theinner membrane that consists, minimally, of three sub-units (TatABC) in Escherichia ... specificity determinant for proteinsecretion via the twin-arginine translocation pathway.J Biol Chem 275, 41350–41357.16 Pop O, Martin U, Abel C & Muller JP (2002) The twin-arginine signal peptide ... TM spans by glycine. The presenceof glycine can also lead to increased flexibility in TMhelices [34]. However, glycine residues can also playimportant roles in modulating inter-helix interactions[35]...
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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx
... changes in the cytoskeleton thatmay occur during the initial 6 h window of infection.We infected RPE cells with K26GFP [27], and stainedcells for F-actin, using phalloidin at 30 min and 6 hpostinfection, ... FEBS 5281damine-conjugated phalloidin (Invitrogen) was added for F-actin staining at room temperature for 45 min. Finally, thecells were washed three times with 1· NaCl ⁄ Pibefore micros-copy ... performed using DAKO Target RetrievalSolution 10· concentrate (DAKO, Carpinteria, CA, USA).Nonspecific staining was blocked using an H2O2solution for 10 min, followed by a protein block for...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... Diego, CA, USA) binding andPI staining were performed according to [44]. Followingincubation with 1.0 lgÆmL)1annexin V and 4.0 lgÆmL)1PI for 20 min, cells were analyzed by flow cytometry.Preparation ... detected by Nile red stain-ing as previously described [42]. Cell imaging for Nile redstaining was performed by confocal microscopy. Quantifica-tion of lipid droplets was performed by flow cytometricanalysis ... variety of celltypes, including hepatocytes, to long-chain saturatedfatty acids (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling,induction of endoplasmic...
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... protein 1 ⁄ core pro-tein 2) in a large core structure that alsoincludes the chaperone protein Bcs1p. Thiscore structure is then able to sequentiallybind the remaining bc1subunits in a ... [45] and the transformantswere then selected for leucine prototrophy. The doubledeletion strains were constructed by crossing selected singledeletion strains. The resulting diploids were sporulated ... mmphenylmethanesulfonyl fluoride] containing 1% digitonin(w ⁄ v) for 10 min at 0 °C. After a clarifying centrifugationat 20 000 g for 30 min (5810R centrifuge, F-45-30-11 rotor)to remove insoluble material,...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... European BioinformaticsInstitute’s server (http://www.ebi.ac.uk/) for clu-stalw2 [53] as Phylip-tree type and neighbour-joiningclustering [26] using the alignment of completesequences, including ... cDNA involved in cystine,dibasic, and neutral amino acid transport. J Clin Invest91, 1959–1963.26 Saitou N & Nei M (1987) The neighbor-joiningmethod: a new method for reconstructing phylogenetictrees. ... for detectingevolutionary forces at a single amino-acid site. Bioin-formatics 21, 2101–2103.39 Da Lage JL, Danchin EG & Casane D (2007) Wheredo animal a-amylases come from? An interkingdomtrip...
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Tài liệu Báo cáo khoa học: Progress for dengue virus diseases Towards the NS2B–NS3pro inhibition for a therapeutic-based approach ppt
... Asynergistic increase in neutralizing antibody titers by Table 1. Vaccines against dengue fever by the Initiative for Vaccine Research, World Health Organization, updated February 2006.Type of vaccinePharmaceuticalcompany ... also for the correct interactionwith the substrate. The binding site of the NS2B cofac-tor could be examined for a view to inhibitor develop-ment, and competitive inhibitors of the binding ... an important aid for patients and for improving survival in severe forms ofdisease. Antiviral therapeutic strategies involve virus-binding blocking to prevent intracellular virus multipli-cation...
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Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... N-ter-minal or C-terminal histidine tags. For that purpose, a cod-ing sequence for six histidines was introduced by PCReither at the N-terminus, after the ATG, or at the C-termi-nus before the ... protein had a similar activity in crude yeastextract as the untagged protein, indicating that the tagwas not interfering with the protein activity. Thetagged protein was purified and then used for ... dl-glyceraldehyde in an extractof H. jecorina was about 3 nkatÆmg)1. The GLD1 pro-tein was tagged with a histidine tag at the N-terminalend by adding the coding sequence for six histidines tothe...
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Tài liệu Báo cáo khoa học: "Sentence-For-Sentence Translation: An Example" ppt
... outlined in Figure 1. Each Arabic letter has several forms. The particular form selected in any given instance is determined by the preceding and following letters. In general, there- fore, in ... input sentence remains intact, in order and form, as it does in the present program. The only changes which are made are in the form of added elements making grammatical information ex- plicit. ... rules. The substructure indicated by the applicable subrule is an MB. The entry in the second column, MB (-all NP) indicates that no information in any noun phrase occurring in any portion of the...
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