Tài liệu Báo cáo khoa học: The kinetic properties of various

Tài liệu Báo cáo khoa học: The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase doc

... of DAOCS by the Kunkel method [20,21]. Mutation Primer sequence R258K 5¢-ACTGGAGGTCTTGCTGCTGC-3¢ R258H 5¢-ACTGGAGGTGTGGCTGCTGC-3¢ R258A 5¢-ACTGGAGGTCGCGCTGCTGC-3¢ R258Q [17] 5¢-ACTGGAGGTCTGGCTGCTGC-3¢ R258L ... Sciences Research Coun- cil, Medical Research Council, the Wellcome Trust, the European Union and the National Science Council, Republic of China are thanked for ﬁnancial a...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... of the mini-scaffoldin [21]. The most active mini-cellulo- some on microcrystalline cellulose was composed of the processive cellulase Cel48F combined with endo- glucanase Cel9G. Adding the C. ... efﬁcient association of components. The possible occurrence of synergies between cellulo- somal fractions which might account for the efﬁciency of the cellulosomes was inves...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: "The Sentimental Factor: Improving Review Classiﬁcation via Human-Provided Information" docx

... d and the logged co-occurence vector N (w,a k ) . In this respect, the vector of logged co-occurrences is used to produce derived feature. 4 Data Analysis 4.1 Accuracy of Unsupervised Coefﬁcients By ... avoid co- occurrences that are primarily relevant to other subjects. • Reproducibility On a ﬁxed corpus, counts of word occurrences produce consistent results. Due to the dynamic n...

Ngày tải lên : 20/02/2014, 16:20

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Tài liệu Báo cáo khoa học: The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis ppt

... characterized lipid ⁄ protein interface of the nAChR as a prototype target site of tadpole narcosis, the microscopic inhibition constants of narcotic chemi- cals need to be known. The K I -value of ... 0.092 a Logarithms of octanol ⁄ water partition coefﬁcient. b Microscopic inhibition constant. c Microscopic lipid dissociation binding constant of a class of more loosely...

Ngày tải lên : 19/02/2014, 18:20

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Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... time constant of the kinetic controls (about 100 s) then the separation of the kinetic and genetic controls (an implicit assumption in our model) would be justiﬁed. The characteristics of the ... allosteric controls of the branch-point enzymes are not taken into account in these experimental studies. Also the occurrence of branch-point two-substrate enzymes and th...

Ngày tải lên : 20/02/2014, 02:21

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Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... to increase the catalytic efﬁciency of PhyH-DII. Further- more, the catalytic efﬁciency of PhyH was much greater than that of PhyH-DI and PhyH-DII combined (5.73 fold) or twice the amount of PhyH-DII ... ( 嘙 C ) Relative activity (%) PhyH PhyH-DII AB CD Fig. 2. Environmental factors that affect the catalytic activities of PhyH and PhyH-DII. (A) Effect of 0–5.0 mM Ca 2+...

Ngày tải lên : 14/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... However, the AGC VIII kinases represent a plant-speci c sub- family characterized by a conserved DFD amino acid motif in subdomain VII of the catalytic domain and by the presence of an amino acid ... addresses Cardiff School of Biosciences, Biomedical Sciences Building, Cardiff, UK àSICIT 2000 S.p.A., Chiampo, Italy §Syngenta Seeds, Enkhuizen, the Netherlands –UMR Ecophysiolog...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... yielding the supernatant LS2, containing the cytosol-enriched fraction, and the pellet LP2, which is enriched in synaptic vesicles. The protein concentrations were measured using the bicinchoninic acid ... location of p25a was in the cytosolic S3 fraction, and only minor amounts of p25a were present in the other fractions. The partial co-fractionation was conﬁrmed by con...

Ngày tải lên : 14/02/2014, 21:20

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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... also include control through p53. Cell cycle arrest can be medi- ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc2 5C. CDE ⁄ CHR-dependent ... combined with the change of protein occupation on the Cdc2 5C CDE, indicates that CDE ⁄ CHR sites which regulate cyclin A, cyclin B and Cdc2 promoters may lose binding of...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... apoptosis. The mitochondrial release of cytochrome c and second mitochondria-derived activator of caspase (Smac ⁄ DIABLO) allows for the formation of the apoptosome, a complex that enables the activation ... produced (Fig. 1D, lane 2). In the presence of lactacystin, a sig- niﬁcant increase in the amount of tBid–GFP present in the extract, both in control conditions...

Ngày tải lên : 16/02/2014, 09:20

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