Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx

... insects. Annu Rev Entomol 51, 1–24. 7 Nagasawa H, Kataoka H, Isogai A, Tamura S, Suzuki A, Mizoguchi A, Fujiwara Y, Suzuki A, Takahashi SY & Ishizaki H (1986) Amino acid sequence of a protho- racicotropic ... concentrated the ELISA- positive material into a peak so sharp that we judged that sufficient purification had been achieved. Determination of the structure of 8K-B...

Ngày tải lên: 18/02/2014, 13:20

... further decrease the stability of the canavany- lated species. The role of tRNA as a cofactor for aminoacylation in those aminoacyl-tRNA synthetases that require tRNA for amino acid activation is ... amino acid utilization by the plant arginyl-tRNA synthetases. The aminoacylation level attained in the pres- ence of L-arginine was compared to that in the presence of 1 m...

Ngày tải lên: 18/02/2014, 13:20

... CGATTTGCTGACCACCTTCT MLL1 GAGGACCCCGGATTAAACAT GGAGCAAGAGGTTCAGCATC MLL2 GTGCAGCAGAAGATGGTGAA GCACAATGCTGTCAGGAGAA MLL3 AAGCAAACGGACTCAGAGGA ACAAGCCATAGGAGGTGGTG MLL4 GTCTATGCGCAGTGGAGACA AGTCTGCATCCCCGTATTTG HOXC13-ERE1 ... TGCCCTCATATAAACCTGGAA AGCCTTTGGGAGTAGGAACC ERa antisense CATGGTCATGGTCAG a ERb antisense GAATGTCATAGCTGA a MLL1 antisense TGCCAGTCGTTCCTCTCCAC a MLL2 antisense ACTC...

Ngày tải lên: 18/02/2014, 14:20

... annotators label examples. Assume that we have an annotator and the learner selects some bunsetsu pair of the j-th bunsetsu and the i-th bun- setsu such that j < i. The annotator is then asked what ... simplicity. 6.3 Features There are features that have been commonly used for Japanese dependency parsing among related papers, e.g., (Kudo and Matsumoto, 2002; Sas- sano, 2004; Iwa...

Ngày tải lên: 20/02/2014, 04:20

... several hundred features. Then we r un our algorithm on the data and eval- uate the precision and recall. We also run the al- gorithms described in Hu and Liu (200 4a) on the same data as the baseline. From ... vocabu- lary of product features. Hierarchy struc- ture information and unit of measurement information are mined from the specifi- cation to improve the accuracy of...

Ngày tải lên: 20/02/2014, 09:20

... self- referential lexical items that are the logical or grammatical subject of a predication that needs not be a complete grammatical sentence. 3 At a very basic semiotic level natural language has ... extraction of metalinguistic information from technical and scientific documents. We claim that such a system can create special databases to boot- strap compilation an...

Ngày tải lên: 20/02/2014, 15:20

... deleted Plasmids transformed into E. coli BL21(DE3) Activity against 2MA atdA1 pACYC A2 and pET A3 A 4A5 – atdA2 pACYC A1 and pET A3 A 4A5 + atdA3 pACYC A1 A2 and pET A4 A5 – Control (no deletion) pACYC A1 A2 ... of AtdA3, the largest substrate accepted by the WT AtdA, 2EA, was docked into the AtdA3 homology model. The approxi- mate initial position of the substrate was d...

Ngày tải lên: 19/02/2014, 02:20

... CCGGTG GTcGAcTTTGGTTtcACGCCC SalI E273Q ACGTACTGCTgTCCGGAA AGtACtCCATGGCCGC ScaI D353N TCCAATCCGTtAGTC TGgCCaTAGAACGCGC MscI E356Q GGAGAAT GGTgCCAGGGTACATTTcCAATCCGTCA KpnI D372N AATACAT CTTaAGGCCGTCGTtGTCGGTGTGG A II D373N ... gnition sites ( u nderlined). Pimers Nucleotide sequence (5¢fi3¢) W31F CTGACCTtcTGGCATAAC ACCGGtGAAATC AgeI W32F CCTGGTtcCATAAC ACCGGtGAAATC AgeI W240F GGTGCT gAGCTC...

Ngày tải lên: 19/02/2014, 16:20

... gctttttggcaccaaaaaggccggctccatcg Leu25 to Ala G3 3A G3 3A F ggttccgatcttgctgcgtcgatcaaagg Gly33 to Ala F3 9A F3 9A F gcgtcgatcaaaggcgctaaaaaagcaatgagcg Phe39 to Ala 3K > Q K37Q F ggtgcgtcgatccaaggctttcaacaagcaatgag ... Ala F9 4A F9 4A F caggtgtgggcagctatcgccccagcgc Phe94 to Ala P9 7A P9 7A F ggcatttatcgccgctgcgctgtataagcatg Pro97 to Ala L9 9A L9 9A F cgccccagcggcgtataagcatgaacgtc...

Ngày tải lên: 19/02/2014, 17:20

... data were captured using a Fuji LAS-1000 Imaging System CCD camera (aida 2.11 soft- ware for analysis). ACE2 ⁄ ACE activity assays Fluorogenic assays using the synthetic ACE2 substrate, Mca-APK(Dnp) ... that the ACE residue His1089, shown to be involved in the Fig. 1. Schematic view of the active site of ACE2 and tACE. Binding interactions of the inhibitor (A) MLN-4760...

Ngày tải lên: 20/02/2014, 01:20