... caused by using a planttranslation system, in which mammalian mRNA wasinappropriately translated. The transient expressionassay indicated that M33S and isoform 1 proteinswere secreted into the ... Mountain View, CA) was used as a template, because this organ abundantly expresses mRNAfor both isoforms 1 and 2. Oligo cap RACE was performedusing a Gene racer kit (Invitrogen, Carlsbad, CA), accord-ing ... 5¢-AGGAACCAAACACCAAGTGG-3¢(Fig. 2A) .Luciferase promoter assayHuman genomic DNA was isolated from two volunteers(both male, Japanese, aged 34 and 37 years) after they gaveinformed consent. A 1460...