... markers; lane 1, culture supernatant of PhyH;lane 2, culture supernatant of PhyH-DII; lane 3, culture supernatantof PhyH-DI; lane 4, purified PhyH; lane 5, purified PhyH-DII; andlane 6, purified ... or an HAP(E. coli AppA) and InsP6reaction mixture (Table 2),Fig. 1. Electrophoretic analysis of PhyH, PhyH-DI and PhyH-DII. (A)SDS ⁄ PAGE analysis of purified PhyH, PhyH-DI and PhyH-DII. LaneM, ... asix-blade b-propeller containing five four-strandedsheets and one five-stranded sheet.Expression and purification of PhyH, PhyH-DI andPhyH-DIIPhyH, PhyH-DI and PhyH-DII were each expressed inEscherichia...