Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx

... phase stages, in the basidiomycetes, Coprinus cinereus. To study DNA poly- merase a during meiosis, we cloned cDNAs for the C. cinereus DNA polymerase a catalytic subunit (p140) and C. cinereus primase ... step of C. cinereus DNA polymerase a. One unit (1 U) of DNA polymerase was defined as the amount needs to catalyze the incorporation of 1 pmol of [ 3 H]-d...

Ngày tải lên: 20/02/2014, 11:20

... 431 directly involved in the change in active form of cas- pases and DNA fragmentation-induced factors [5,11]. The induction of DNA fragmentation is closely linked to the phosphorylation of proteins such ... by containing a Ser139 phosphorylation site in the C-terminal tail. Phosphorylation of H2AX on Ser139 is a key event in the repair of DNA damage and the induction...

Ngày tải lên: 14/02/2014, 22:20

... detectable binding to CRP at the concentration and during the period of observation used in the experiment. Interaction analyses using increasing concentrations of the native and of the low-pH ... same antigen– antibody interaction in the presence of increasing concentrations of NaCl. In both experiments, binding intensities are represented in RU, and each data point shows...

Ngày tải lên: 16/02/2014, 15:20

... alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXR receptors. This ... 57, 858–865. 36 Nakagawa Y, Minakuchi C, Takahashi K & Ueno T (2002) Inhibition of [3H] ponasterone A binding by ecdysone agonists in the intact Kc cell line. Insect Bio- chem...

Ngày tải lên: 18/02/2014, 08:20

... 56% in the dark (Fig. 7A). The total amount of alanine, serine and gly- cine was reduced from 33% in the light to 25% in the dark (Fig. 7A). Glutamine and asparagine were the major amino acids in ... The kinetics of Fd-GOGAT activity were assayed by increasing the concentrations of Fd isoprotein as indicated in the figure (C). Photosynthetic (s) and nonphotosynthetic (d) combin...

Ngày tải lên: 18/02/2014, 18:20

... or cofactor. In detail, the N-terminal module is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the third domain binds CH 3 -Cbl; and the fourth domain ... the interactions occurring at the binding site, and to develop a tool that could assist further optimization of inhibitors, a molecular model of the methyltetra- hydrofolate-binding domain o...

Ngày tải lên: 19/02/2014, 05:20

... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl-X L expression, ... 1–122), but retains the DNA binding and leucine-zipper region of c-Jun, it should function as a dominant-negative mutant of c-Jun to block wild-type c-Jun bindin...

Ngày tải lên: 19/02/2014, 06:20

... structure determinants of the nonenzymatic deamidation of asparagine and glutamine residues in proteins. Protein Eng. 4, 283–294. 36. Tyler-Cross, R. & Schirch, V. (1991) Effects of amino acid sequence, ... The observation of species of increasing mass spaced by 162 Da reflects the presence of glycoforms with an increasing number of hexose (probably mannose) units. Phosphory...

Ngày tải lên: 19/02/2014, 12:20

... the interdigital regions of limbs, suggesting that Hsp105a may play important roles in apoptosis dur- ing mouse embryogenesis [27]. In the present study, to examine the role of Hsp105a during ... the activation of caspases. These enzymes participate in a cascade that is triggered in response to pro-apoptotic signals and results in cleavage of a set of proteins, resulting...

Ngày tải lên: 21/02/2014, 03:20

... meta- bolism involved in the events underlying in ammation, such observations further underscore an emerging con- cept suggesting that modulation of the 12-LOX path- way during intestinal in ammation ... steps during this recruitment process. In a scenario similar to the intes- tine, during bacterial infection of the lung, such as P. aeruginosa infection, IL-8 probably plays a...

Ngày tải lên: 19/02/2014, 02:20