Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-

Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx

... phase stages, in the basidiomycetes, Coprinus cinereus. To study DNA polymerase a during meiosis, we cloned cDNAs for the C. cinereus DNA polymerase a catalytic subunit (p140) and C. cinereus primase ... step of C. cinereus DNA polymerase a. One unit (1 U) of DNA polymerase was deﬁned as the amount needs to catalyze the incorporation of 1 pmol of [ 3 H]-d...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... 431 directly involved in the change in active form of caspases and DNA fragmentation-induced factors [5,11]. The induction of DNA fragmentation is closely linked to the phosphorylation of proteins such ... by containing a Ser139 phosphorylation site in the C-terminal tail. Phosphorylation of H2AX on Ser139 is a key event in the repair of DNA damage and the induction...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modiﬁcations and in enhanced protective activity in sepsis doc

... detectable binding to CRP at the concentration and during the period of observation used in the experiment. Interaction analyses using increasing concentrations of the native and of the low-pH ... same antigen– antibody interaction in the presence of increasing concentrations of NaCl. In both experiments, binding intensities are represented in RU, and each data point shows...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXR receptors. This ... 57, 858–865. 36 Nakagawa Y, Minakuchi C, Takahashi K & Ueno T (2002) Inhibition of [3H] ponasterone A binding by ecdysone agonists in the intact Kc cell line. Insect Bio- chem...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

... 56% in the dark (Fig. 7A). The total amount of alanine, serine and gly- cine was reduced from 33% in the light to 25% in the dark (Fig. 7A). Glutamine and asparagine were the major amino acids in ... The kinetics of Fd-GOGAT activity were assayed by increasing the concentrations of Fd isoprotein as indicated in the ﬁgure (C). Photosynthetic (s) and nonphotosynthetic (d) combin...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx

... or cofactor. In detail, the N-terminal module is the homocysteine (Hcy)-binding domain; the second domain binds 5-methyltetrahydrofolate, the third domain binds CH 3 -Cbl; and the fourth domain ... the interactions occurring at the binding site, and to develop a tool that could assist further optimization of inhibitors, a molecular model of the methyltetrahydrofolate-binding domain o...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl-X L expression, ... 1–122), but retains the DNA binding and leucine-zipper region of c-Jun, it should function as a dominant-negative mutant of c-Jun to block wild-type c-Jun bindin...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khóa học: Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei ) Cel7B catalytic module pptx

... structure determinants of the nonenzymatic deamidation of asparagine and glutamine residues in proteins. Protein Eng. 4, 283–294. 36. Tyler-Cross, R. & Schirch, V. (1991) Eﬀects of amino acid sequence, ... The observation of species of increasing mass spaced by 162 Da reﬂects the presence of glycoforms with an increasing number of hexose (probably mannose) units. Phosphory...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... the interdigital regions of limbs, suggesting that Hsp105a may play important roles in apoptosis during mouse embryogenesis [27]. In the present study, to examine the role of Hsp105a during ... the activation of caspases. These enzymes participate in a cascade that is triggered in response to pro-apoptotic signals and results in cleavage of a set of proteins, resulting...

Ngày tải lên : 21/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Bacterial-induced hepoxilin A3 secretion as a pro-inﬂammatory mediator pptx

... metabolism involved in the events underlying in ammation, such observations further underscore an emerging con- cept suggesting that modulation of the 12-LOX pathway during intestinal in ammation ... steps during this recruitment process. In a scenario similar to the intes- tine, during bacterial infection of the lung, such as P. aeruginosa infection, IL-8 probably plays a...

Ngày tải lên : 19/02/2014, 02:20

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