Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt
Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt
... engineered ribozyme for RNA sequence exchange S. A. Ivanov et al.4474 FEBS Journal 272 (2005) 4464–4474 ª 2005 FEBS Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes ... site-specific alteration of RNA sequence is demonstrated.Results RNA ligation by reverse-joined hairpin ribozymes Reverse-joined hairpin ribozymes, first introduced by Ohtsuka and colleagues [29,30], ... variants of atandem configured hairpin ribozyme. Experimental proceduresSynthesis of ribozymes and substrates Reverse-joined hairpin ribozymes HP–RJWTAn (n ¼ 6, 7,8, 12) and the twin ribozyme...
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Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... control ODN (con) and the dead cells were counted after 16, 24 and 48 h of culture using trypan blue staining. (C) Cells weretreated with 0.5, 1 and 2 lg of hpST3dODN and 1 and 2 lg of control ODN ... activated [26] and found that SW480 cells wereefficiently killed by the hpST3dODN. SW480 cellswere also efficiently killed by IFN-c treatment, and this action was counteracted by hpST3dODN, whichreduced ... 2505–2515 ª 2009 The Authors Journal compilation ª 2009 FEBS 2515 Efficient killing of SW480 colon carcinoma cells by asignal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide...
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Tài liệu Báo cáo khoa học: "Efficient Online Locality Sensitive Hashing via Reservoir Counting" ppt
... have a reservoir of size 5, containingthree values of −1, and two values of 1, then the exchangeabil-ity of the elements means the reservoir is fully characterized by knowing k, and that there ... various values of s and u.Algorithm 1 RESERVOIRUPDATE(n, k, m, σ, s)Parameters:n : size of stream so fark : size of reservoir, also maximum value of sm : magnitude of updateσ : sign of updates ... semantics (Bhagat and Ravichandran, 2008;Van Durme and Lall, 2009; Pantel et al., 2009; Linet al., 2010; Goyal et al., 2010; Bergsma and VanDurme, 2011), as well as large-scale processing of social...
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Tài liệu Báo cáo khoa học: Transient DNA ⁄ RNA-protein interactions docx
... themechanism by which the ribosome attains its high level of accuracy in translation, its catalytic triad (rRNA,ribosomal protein, and the peptidyl-tRNA substrate), and the mode of action of many ... Structure of the 70S ribosome. The structure of the ribosome of Thermus thermophilus (Protein Data Bank entries 1GIX and 1GIY) isshown, with the rRNA molecules represented by thin coils, the tRNAs by ... structure of the ‘glutaminetransamidosome’ of Thermotoga maritima [22] showsthat the anticodon-binding domains of the synthaserecognizes the common features of tRNAGln and tRNAGlu(the second and...
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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf
... analysed by RNase protection assay or by northern analysis (10 lgoftotal RNA) using the intron spanning32P-labelled globin and GAPDH probes described above. The b-globin and GAPDH mRNA band intensities ... of the ARE I-AUUUA and ARE III-AUUUAsequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-richsequence used primers SJS259 and SJS260, and ... charac-terization and importance in mRNA degradation.Trends Biochem Sci 20, 465–470.36 Wilson T & Treisman R (1988) Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3¢ AU-rich...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... commoncontaminants of TAP assays [43,44]. On the otherhand, the presence of ribosomal subunits in the TIAR-TAP pellet might result from uncompleted RNA deg-radation by RNAse A in the purification process and reflect ... 2% SDS) and twice extracted with phenol ⁄ chloroform, once extractedwith chloroform, and precipitated with ethanol and sodiumacetate [62]. The quality of the RNA samples was verified by agarose ... effect of ASF ⁄ SF2-CP on cyto-plasmic Rluc mRNA accumulation was measured and, to normalize the transfection and recovery efficiencies,the accumulation of Fluc mRNA was measured in thesame RNA...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... pro-teins P40 and P90. Absence of these proteins causes arandom insertion of the P1 protein and a cytadher-ence-negative phenotype [13]. P1 is encoded by MPN141 and both, P40 and P90 by MPN142. ... theN-terminus of P40 as it appears in protein extracts of M. pneumoniae.Determination of the N-terminus of P40 by Edmandegradation failed due to the limited amount of start-ing material. An alternative ... freeamino terminal groups of the peptides are sulfonated by the CAF reagent (Fig. 2).Subsequently, the protein was digested by trypsin and the supernatant was analyzed by ESI-QTOF MS(Fig. 3) in the...
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Tài liệu Báo cáo khoa học: Enlarging the gas access channel to the active site renders the regulatory hydrogenase HupUV of Rhodobacter capsulatus O2 sensitive without affecting its transductory activity docx
... activity and rate of H2 and HD formation by wild-type and mutated HupUV proteins of R. capsulatus. The values areinitial rates corrected for gas consumption by the mass spectrometer. Activity and ... with a Featom liganded by 1 CO and 2 CN–[16], and the binding of an hydride to Ni and Fe after H2reduction hasrecently been demonstrated [38]. However, in contrastwith standard hydrogenases, ... sub-unit of D. fructosovorans hydrogenase) of the largesubunit (HupV) of HupUV with Val and Leu, respect-ively, and showed that these amino acids are indeedinvolved in the O2insensitivity of the...
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Tài liệu Báo cáo khoa học: "Towards Tracking Semantic Change by Visual Analyti cs" docx
... visualexploration of semantic change.1 IntroductionThe problem of determining and inferring the sense of a word on the basis of its context has been thesubject of quite a bit of research. Earlier ... the details of thedata by zooming in on the occurrences of particu-lar words in their contexts. This makes it possibleto compensate for one of the major disadvantages of generative and vector ... modelsare capable of clustering and disambiguat-ing senses, a more recent trend investigateswhether changes in word meaning can betracked by automatic methods. The aim of ourstudy is to offer a new...
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Tài liệu Báo cáo khoa học: "REPAIRING REFERENCE IDENTIFICATION FAILURES BY RELAXATION" doc
... selected by the partial marcher as potent~a| candidates for the referent. These candidates are shown across the top of the figure. The lines on the right side of each box correspond to the set of ... simplified and hnearlzed vlew of the actual KL-One representatlon of the speaker's descriptions after they have been parsed and semantically interpreted. A representation of each of the ... kind of Shape ROUND; (>) o the recurswe partial matching of zubparts. BASE in Descr2 could match to the subpart (n STAND that has a Translation of 10.0 00 0.0) - I.e Base of STAND....
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