Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... engineered ribozyme for RNA sequence exchange S. A. Ivanov et al. 4474 FEBS Journal 272 (2005) 4464–4474 ª 2005 FEBS Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes ... site-specific alteration of RNA sequence is demonstrated. Results RNA ligation by reverse-joined hairpin ribozymes Reverse-joined hairpi...

Ngày tải lên: 20/02/2014, 01:20

... control ODN (con) and the dead cells were counted after 16, 24 and 48 h of culture using trypan blue staining. (C) Cells were treated with 0.5, 1 and 2 lg of hpST3dODN and 1 and 2 lg of control ODN ... activated [26] and found that SW480 cells were efficiently killed by the hpST3dODN. SW480 cells were also efficiently killed by IFN-c treatment, and this action was counte...

Ngày tải lên: 18/02/2014, 08:20

... have a reservoir of size 5, containing three values of −1, and two values of 1, then the exchangeabil- ity of the elements means the reservoir is fully characterized by knowing k, and that there ... various values of s and u. Algorithm 1 RESERVOIRUPDATE(n, k, m, σ, s) Parameters: n : size of stream so far k : size of reservoir, also maximum value of s m : magnitude of...

Ngày tải lên: 20/02/2014, 04:20

... the mechanism by which the ribosome attains its high level of accuracy in translation, its catalytic triad (rRNA, ribosomal protein, and the peptidyl-tRNA substrate), and the mode of action of many ... Structure of the 70S ribosome. The structure of the ribosome of Thermus thermophilus (Protein Data Bank entries 1GIX and 1GIY) is shown, with the rRNA molecules represented...

Ngày tải lên: 14/02/2014, 19:20

... analysed by RNase protection assay or by northern analysis (10 lgof total RNA) using the intron spanning 32 P-labelled globin and GAPDH probes described above. The b-globin and GAPDH mRNA band intensities ... of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-rich sequence used pr...

Ngày tải lên: 16/02/2014, 09:20

... common contaminants of TAP assays [43,44]. On the other hand, the presence of ribosomal subunits in the TIAR- TAP pellet might result from uncompleted RNA deg- radation by RNAse A in the purification process and reflect ... 2% SDS) and twice extracted with phenol ⁄ chloroform, once extracted with chloroform, and precipitated with ethanol and sodium acetate [62]. The quality of...

Ngày tải lên: 16/02/2014, 15:20

... pro- teins P40 and P90. Absence of these proteins causes a random insertion of the P1 protein and a cytadher- ence-negative phenotype [13]. P1 is encoded by MPN141 and both, P40 and P90 by MPN142. ... the N-terminus of P40 as it appears in protein extracts of M. pneumoniae. Determination of the N-terminus of P40 by Edman degradation failed due to the limited amount...

Ngày tải lên: 19/02/2014, 18:20

... activity and rate of H 2 and HD formation by wild-type and mutated HupUV proteins of R. capsulatus. The values are initial rates corrected for gas consumption by the mass spectrometer. Activity and ... with a Fe atom liganded by 1 CO and 2 CN – [16], and the binding of an hydride to Ni and Fe after H 2 reduction has recently been demonstrated [38]. However, in contra...

Ngày tải lên: 20/02/2014, 01:20

... visual exploration of semantic change. 1 Introduction The problem of determining and inferring the sense of a word on the basis of its context has been the subject of quite a bit of research. Earlier ... the details of the data by zooming in on the occurrences of particu- lar words in their contexts. This makes it possible to compensate for one of the major disadvantages...

Ngày tải lên: 20/02/2014, 05:20

... selected by the partial marcher as potent~a| candidates for the referent. These candidates are shown across the top of the figure. The lines on the right side of each box correspond to the set of ... simplified and hnearlzed vlew of the actual KL-One representatlon of the speaker's descriptions after they have been parsed and semantically interpreted. A representa...

Ngày tải lên: 21/02/2014, 20:20