Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... enrichment in iNOS activity within the particulate fraction of epithelial cells. Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, experiments were con- du...

Ngày tải lên: 19/02/2014, 16:20

... level of ROS in the medium of HepG2 cells exposed to hydrogen peroxide. HepG2 cells were incu- bated with 5.5 m M glucose in the presence of 0.8 mM pyruvate, and exposed to increasing doses of hydrogen ... not shown). This is in line with our previous work [29] indicating enhancement of glycolysis activity in the presence of pyruvate. Increased glycolysis might thus...

Ngày tải lên: 18/02/2014, 16:20

... (CAF) [30]: during the first step of the labeling reaction the e-amino groups of lysine are specifically converted to homoarginine. In a second step, the free amino terminal groups of the peptides ... function of this protein com- plex, the subunits had to be characterized in detail. The N-terminal end of P90 was determined by Edman degradation. This protein begins with an arginin...

Ngày tải lên: 19/02/2014, 18:20

... STRUCTURES 227 DISTRIBUTION OF NON-PRONOMINAL AND PRONOMINAL NOUN PHRASES Non-pronominal noun phrases play a role in indicating and realizing topic shifts in a dialogue. Since new subdialogues ... realizing topic continuity in a dialogue. Since new topics are introduced at the beginning of new subdialogues and developed in the following sentences, one can predict that pro...

Ngày tải lên: 21/02/2014, 20:20

... replace- ment of lysine residues by arginine or d-lysine residues in the polybasic domain of K-Ras does not interfere with plasma membrane localization of this protein [30], suggesting that binding of K-Ras ... incubated in the presence of monensin, plasma membrane targeting of K-Ras was not altered (Fig. 7), thus ruling out a possible role of H + in intracellular transpor...

Ngày tải lên: 19/02/2014, 00:20

... and anaerobic condi- tions, in the range of 0.6 lmolÆmg )1 Æmin )1 . No evidence was obtained for redox cycling of FMN. The amino acid residues involved in the FMN binding site are absolutely conserved ... M15-pQEidi2. Preparation of the recombinant Idi-2 protein The recombinant E. coli strain M15-pQEidi2 was grown in Luria-Bertani broth containing ampicillin (180 mgÆL )1 )and kan...

Ngày tải lên: 19/02/2014, 13:20

... for glyphosate activity is accompanied by improved bind- ing to longer ligands (up to five atoms in the main chain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 ... d-amino acid oxidase [47]. In B. subtil- is, GO is involved in biosynthesis of the thiazole moiety of thiamine pyrophosphate (vitamin B 1 ). This reaction requires the dir...

Ngày tải lên: 14/02/2014, 14:20

... that tau interacts with Fyn-SH2. Proteins harbouring SH2 domains bind to phosphorylated tyrosines on their tar- get proteins, thereby coupling the activity of tyrosine kinases with intracellular ... interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform of tau in the human central nervous system (CNS). Two distinct PXXP motifs in...

Ngày tải lên: 14/02/2014, 14:20

... observe any trace of the movements on the His98 residue in our MaL ⁄ rMaL Fig. 2. Stabilizing carbohydrates of TaLcc1. Domain A is presented in blue, domain B in green, and domain C in yellow. (A) ... mutation, suggesting the importance of the carboxylic group for the catalytic activity [46]. In addition, Phe427 in rMaL (Val428 in TaLcc1) might be involved in placing s...

Ngày tải lên: 14/02/2014, 18:20

... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4 NLS peptide. The NLS peptide binds to the major binding site in an extended ... The importin-b binding domain is known to have an autoinhibitory function, whereby an internal NLS-like sequence competes for the importin-a binding site, reducing binding affinity...

Ngày tải lên: 14/02/2014, 19:20