Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... Insights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase Hiromi Akeboshi1, ... doi:10.1111/j.1432-1033.2004.04378.x[10,17]. D espite these a dvances, little is known a bout the unique substrate preference and the catalytic mechanism of IPU.To clarify the catalytic mechanism of IPU, site-directed mutagenesis was ... the negatively numberedsubsites, and the substrate preference of the negativelynumbered subsites of IPU also resembles that of GH family 49 dextranases.Materials and methodsHost strains and...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected byhand. Since the sequences of the two arginases and agmatinase greately differ in the ... one of the manganeseions. This, together with the fact that the positions of otherFig. 4. A superimposition of the structures of B. caldovelox arginase(red), rat liver arginase (yellow) and the ... the environ-ment of tryptophan residues is essentially conserved in the mutant enzyme. On the other hand, the absence of majordifferences in the CD spectra of the wild-type and D153Nagmatinases...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... [18], cowpea[19], and poppy [20,21].In the present study, the first insights into the secon-dary and tertiary structure of PLD a2 from cabbageare given. The surface structure of the enzyme wasinvestigated ... C2domain and four conserved regions (I–IV) specific for the PLD superfamily, where regions II and IV are twocopies of the HxKx4Dx6GSxN (HKD) motif [10,11]. The H, K and D residues of theses ... illustrates the quality of the experimental data(Fig. 2B).Reconstruction of the overall shape of the PLDa2from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studiesAlteration of substrate specificity by replacement of Asn 149 with ... elec-trostatic repulsion between the a-carboxyl group of the amino acid and the introduced aspartic residue atposition 149. On the other hand, as agmatine lacks the a-carboxyl group, there would be no impedimentfor ... for the differences between the activesites of this enzyme and arginase I. In spite of the rel-atively low agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of...
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Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc
... iden-tify the nature of the enzyme and provide additionalinformation about the evolution of the ChEs, including the origins of the acyl pocket, the T exon, and the asymmetric forms of ChE in the vertebrates.Results The ... illustrates the differences between the construction of the acyl pocket in vertebrate and invertebrate AChEs. The line separates the vertebrate and invertebrate AChEs. The numbers at the top of the figure ... assemble the catalytic tetramers of the asymmetric forms. The A12form consists of three suchtetramers attached to the triple-stranded helix of ColQ.This result is the first demonstration of the...
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Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity pdf
... fact, the bulk of the proteolytic processing of the proforms of the SVMPs occurs in advance of release of mature secre-tory vesicle contents into the lumen of the venom gland.In the case of the ... associated with the structure of the adjacentproteinase and disintegrin-like domains and the pack-ing of those two domains together in the structure.Comparison of the sequences in the spacer domainamong ... biosynthetic pathways from transcription at the ER surface, through the endoplasmic reticulumto the Golgi, and release of the secretory vesicles into the venom lumen for the production of the...
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Tài liệu Báo cáo khoa học: "Insights into Non-projectivity in Hindi" docx
... classify these instances based onfactors leading to non-projectivity and presentour analysis of them. For each of these classes,we look at the rigidity of these non-projectiveconstructions and their ... isa chunk and the edge represents the relations be-tween the connected nodes labeled with the karakaor other relations. All the modifier-modified rela-tions between the heads of the chunks ... where the mainverb is the central binding element of the sentence. The arguments including the adjuncts are anno-tated taking the meaning of the verb into consid-eration. The participants in...
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Tài liệu Báo cáo khoa học: "Parsing into Discourse Object Descriptions" pptx
... clarifications and other information that the system might need, and (d) to provide help for the user. In this paper some consequences of these demands for the representation and interaction of various ... objects of the universe of discourse. (2) Different types of knowledge, including object type knowledge and knowledge of the current state of the discourse must be used and integrated in the construction ... evoked by the constituents of a discourse, they and their relations then constituting the discourse model of a speaker. Hayes (1984) refers to the objects, events, commands, states (and so on)...
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Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx
... analysis of the T-DNA insertion for the helps mutant and OE lines of AtHELPS. (A) The conservedmotifs of DExD ⁄ H-box RNA helicases in AtHELPS. Numbers represent the amino acid position of the AtHELPS ... the molecular mechanisms of the regulation of AtHELPS expression, we first performedgenevestigator analysis (http://www.genevestigator.ethz.ch/). The results showed that the expression of AtHELPS ... suggested that AtHELPS is mainly expressedin young seedlings and vascular tissues of leaves, suchas the midrib of the cotyledon, the hypocotyl, and the root vasculature (Fig. 2E). When the plants...
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... explain the dimerization of the bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural ... bc1sub-complex, but the presence of only one of these two subunits does not substitutefor the other. After the addition of ISP, the binding of the last subunit (i.e. Qcr10p) finally occurs. These find-ings ... specificallyrequired for the insertion of ISP into an immature bc1complex and that the association of the Qcr9p subunitwith the bc1complex precedes the binding of ISP. Infact, the direct absence of ISP (DISP),...
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Từ khóa: chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngThơ nôm tứ tuyệt trào phúng hồ xuân hươngGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ