Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... Insights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase Hiromi Akeboshi 1 , ... doi:10.1111/j.1432-1033.2004.04378.x [10,17]. D espite these a dvances, little is known a bout the unique substrate preference and the catalytic mechanism of IPU. To clarify the catalyt...

Ngày tải lên: 19/02/2014, 16:20

... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected by hand. Since the sequences of the two arginases and agmatinase greately differ in the ... one of the manganese ions. This, together with the fact that the positions of other Fig. 4. A superimposition of the structures of B. caldovelox arginase (re...

Ngày tải lên: 21/02/2014, 01:21

... [18], cowpea [19], and poppy [20,21]. In the present study, the first insights into the secon- dary and tertiary structure of PLD a2 from cabbage are given. The surface structure of the enzyme was investigated ... C2 domain and four conserved regions (I–IV) specific for the PLD superfamily, where regions II and IV are two copies of the HxKx 4 Dx 6 GSxN (HKD) motif...

Ngày tải lên: 19/02/2014, 02:20

... Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn 149 with ... elec- trostatic repulsion between the a-carboxyl group of the amino acid and the introduced aspartic residue at position 149. On the other hand, as agmatine...

Ngày tải lên: 20/02/2014, 02:21

... iden- tify the nature of the enzyme and provide additional information about the evolution of the ChEs, including the origins of the acyl pocket, the T exon, and the asymmetric forms of ChE in the vertebrates. Results The ... illustrates the differences between the construction of the acyl pocket in vertebrate and invertebrate AChEs. The line separa...

Ngày tải lên: 18/02/2014, 17:20

... fact, the bulk of the proteolytic processing of the proforms of the SVMPs occurs in advance of release of mature secre- tory vesicle contents into the lumen of the venom gland. In the case of the ... associated with the structure of the adjacent proteinase and disintegrin-like domains and the pack- ing of those two domains together in the struct...

Ngày tải lên: 18/02/2014, 18:20

... classify these instances based on factors leading to non-projectivity and present our analysis of them. For each of these classes, we look at the rigidity of these non-projective constructions and their ... is a chunk and the edge represents the relations be- tween the connected nodes labeled with the karaka or other relations. All the modifier-modified rela- tions betwe...

Ngày tải lên: 20/02/2014, 09:20

... clarifications and other information that the system might need, and (d) to provide help for the user. In this paper some consequences of these demands for the representation and interaction of various ... objects of the universe of discourse. (2) Different types of knowledge, including object type knowledge and knowledge of the current state of the disco...

Ngày tải lên: 22/02/2014, 10:20

... analysis of the T-DNA insertion for the helps mutant and OE lines of AtHELPS. (A) The conserved motifs of DExD ⁄ H-box RNA helicases in AtHELPS. Numbers represent the amino acid position of the AtHELPS ... the molecular mechanisms of the regulation of AtHELPS expression, we first performed genevestigator analysis (http://www.genevestigator. ethz.ch/). The results...

Ngày tải lên: 14/02/2014, 18:20

... explain the dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural ... bc 1 sub-complex, but the presence of only one of these two subunits does not substitute for the other. After the addition of ISP, the binding of the l...

Ngày tải lên: 18/02/2014, 08:20